LOCUS       sequence-148382-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression plasmid for human histone H4 with C-terminal 
            BirA-biotinylation signal and TEV celavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hH4-AVITEV
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             325..348
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             355..375
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             388..432
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     471..487
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     472..488
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(667..684)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 aatcccacca tgagcggcag aggcaagggc ggcaagggac tgggaaaagg cggagccaag
       61 cggcaccgga aggtgctgag agacaacatc cagggcatca ccaagcccgc catcagacgg
      121 ctggctagaa gaggcggcgt gaagagaatc agcggcctga tctacgaaga gacacggggc
      181 gtgctgaagg tgttcctgga aaacgtgatc cgggacgccg tgacctacac cgagcacgcc
      241 aagagaaaga ccgtgaccgc catggacgtg gtgtacgccc tgaagagaca gggcagaacc
      301 ctgtacggct tcggcggcta cgtagattac aaggatgacg acgataagct tgtagagaac
      361 ctgtacttcc agggcgttaa cggcggtggc ctgaacgata tcttcgaggc acagaaaatc
      421 gaatggcatg aggttaactg agcgatcgct tataagcggc cgcgtcgagg tcgaggtcga
      481 cggtatcgat aagcttgata tcgaattccg cccccccccc taacgttact ggccgaagcc
      541 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
      601 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
      661 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
      721 tggaagcttc ttgaagacaa aca
//
