LOCUS       sequence-148383-         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression plasmid for human histone H4 with C-terminal 
            BirA-biotinylation signal and TEV celavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hH4-AVITEV
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(72..88)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(73..89)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(128..172)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(185..205)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(212..235)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 caccggcctt attccaagcg gcttcggcca gtaacgttag gggggggggc ggaattcgat
       61 atcaagctta tcgataccgt cgacctcgac ctcgacgcgg ccgcttataa gcgatcgctc
      121 agttaacctc atgccattcg attttctgtg cctcgaagat atcgttcagg ccaccgccgt
      181 taacgccctg gaagtacagg ttctctacaa gcttatcgtc gtcatccttg taatctacgt
      241 agccgccgaa gccgtacagg gttctgccct gtctcttcag ggcgtacacc acgtccatgg
      301 cggtcacggt ctttctcttg gcgtgctcgg tgtaggtcac ggcgtcccgg atcacgtttt
      361 ccaggaacac cttcagcacg ccccgtgtct cttcgtagat caggccgctg attctcttca
      421 cgccgcctct tctagccagc cgtctgatgg cgggcttggt gatgccctgg atgttgtctc
      481 tcagcacctt ccggtgccgc ttggctccgc cttttcccag tcccttgccg cccttgcctc
      541 tgccgctcat ggtgggattt aaatccgcgg ccgcagatct aattccggcg cctagagaag
      601 gagtgagggc tggataa
//
