LOCUS       sequence-148388-         534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc2/C(1-686, RIT) 
            (without C-terminal region, with RIT mutation abrogating AP1 
            binding) with C-terminal BirA-biotinylation signal and TEV celavage 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV
FEATURES             Location/Qualifiers
     source          1..534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(77..93)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(78..94)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(133..177)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(190..210)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(217..240)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggcggaat
       61 tcgatatcaa gcttatcgat accgtcgacc tcgacctcga cgcggccgct tataagcgat
      121 cgctcagtta acctcatgcc attcgatttt ctgtgcctcg aagatatcgt tcaggccacc
      181 gccgttaacg ccctggaagt acaggttctc tacaagctta tcgtcgtcat ccttgtaatc
      241 tacgtacgtg ggctccgtct tgatggctgg gactgggtgg taggtaaagt gctgaggctg
      301 acttcgtttt ctcttcccat tgatgacgta gaagttcact tttacaggtg tgcggatatg
      361 cttgttccga tattcaggga tctcaacaaa aagcatgttg ggctggctct tgtccttatc
      421 caccgtggct tccatctccc aaatttgctg tccatctgtg gtcttctcag taaacacaac
      481 tttggactcg gatgtaaagt tctgccccgt gaggatcatt tgctggccgc cata
//
