LOCUS       sequence-148526-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian intracellular expression of fluorogen activating protein 
            Mars1Cy and eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Zhang M, Chakraborty SK, Sampath P, Rojas JJ, Hou W, Saurabh S, 
            Thorne SH, Bruchez MP, Waggoner AS
  TITLE     Fluoromodule-based reporter/probes designed for in vivo fluorescence
            imaging.
  JOURNAL   J Clin Invest. 2015 Oct 1;125(10):3915-27. doi: 10.1172/JCI81086. 
            Epub 2015 Sep 8.
  PUBMED    26348895
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI81086"; 
            journalName: "J Clin Invest"; date: "2015-10-1- 1"; volume: "125"; 
            issue: "10"; pages: "3915-27"
COMMENT     Sequence Label: pP2AeGFP-Mars1Cy
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(875..931)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(995..1016)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatcagttat ctagatccgg tggatcccgg gttaggatag tacggtcacc ttggtccctc
      181 cgccgaagaa aacgtgggaa ccactgctgt ctgctgattg acagtaatag tcagcctcgt
      241 cttctgcctg gactccactg atggtcaatg tggctgttgt ccctgaactg gtaccagaga
      301 atcgctcagg gattcctgag ggcctctcag tgtctttata tatcaccaag acaggggcct
      361 ggcctgcctt ctgctggtac caataagtat attgctttgg caatgcatct ccagagcagg
      421 tgatcctggc cgtctgtcct ggggacactg acaccgaggg tggctgagtc aactcatagg
      481 aacctccacc agaacctccg ccagaacctc cacctgaacc gccacctggg cctcctccac
      541 cagacaaaac agtaacttta gtaccaccac cgaaaaatac atgagaacca gaagaatcag
      601 cagattgaca ataataatca gcttcatctt cagcttgaac accagaaata gtcaaagtag
      661 cagtagtacc agaagaagta cctgagaatc tttctggaat accagatggt ctttcagtat
      721 ctttataaat aaccaaaact ggagcttgac cagctttttg ttgataccag taagtgtatt
      781 gttttggcaa agcatcacca gaacaagtaa ttctagcagt ttgacctgga gaaacagaaa
      841 cagatggagg ttgtgttaat tcgtaagatc cgccaggtcc agggttctcc tccacgtctc
      901 cagcctgctt cagcaggctg aagttagtag ctccgcttcc actagtaccg cccttgtaca
      961 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     1021 cgcgcttctc gttggggtct ttgctcaggg cg
//
