LOCUS       sequence-148530-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R 
            fast-cycling mutant) fused to mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL
  TITLE     Optimized second-generation CRY2-CIB dimerizers and photoactivatable
            Cre recombinase.
  JOURNAL   Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.
  PUBMED    27065233
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063."
COMMENT     Sequence Label: CRY2PHR(W349R)-mCherry
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    366..487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(403..422)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     457..476
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(631..652)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
       61 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      121 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      181 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      241 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
      301 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      361 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      421 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      481 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
      541 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
      601 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
      661 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
      721 gaaccatcac cctaatcaag tttt
//
