LOCUS       sequence-148535-       11748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector encoding Mars1Cy-tagged AVPR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Zhang M, Chakraborty SK, Sampath P, Rojas JJ, Hou W, Saurabh S, 
            Thorne SH, Bruchez MP, Waggoner AS
  TITLE     Fluoromodule-based reporter/probes designed for in vivo fluorescence
            imaging.
  JOURNAL   J Clin Invest. 2015 Oct 1;125(10):3915-27. doi: 10.1172/JCI81086. 
            Epub 2015 Sep 8.
  PUBMED    26348895
REFERENCE   2  (bases 1 to 11748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI81086"; 
            journalName: "J Clin Invest"; date: "2015-10-1- 1"; volume: "125"; 
            issue: "10"; pages: "3915-27"
COMMENT     Sequence Label: pLenCMV-PMMars1CyAVPR2
FEATURES             Location/Qualifiers
     source          1..11748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2662..2965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2966..3169
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3208..3217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3214..3273
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             3277..3303
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5216..5780
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5375..5392)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5602..5621
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5813..6412
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5813..5832)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6309..6329
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6440..7028
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6493..6513)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6911..6922)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7031..7047)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7032..7048)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7553..7733
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7759..7776)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7765..7989
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8035..8463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8122..8141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8332..8353
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8472..8492)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8477..8806
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8657..8792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8719..8738
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8854..8901
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8920..9294
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9424..9545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9461..9480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9515..9534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9594..9610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9594..9610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9607..9629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9618..9634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9642..9648,9649..9666,9667..9672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9687..9708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9825..9842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9996..10584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10076..10095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10755..11546,11547..11615))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11378..11397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11616..11720)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atagttatta atagtaatca attacggggt cattagttca
     2641 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2701 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2761 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2821 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2881 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2941 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3001 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3061 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     3121 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     3181 ccgtcagatc cgctagcgct accggtcgcc accatggaga cagacacact cctgctatgg
     3241 gtactgctgc tctgggttcc aggttccact ggtgactatc catatgatgt tccagattat
     3301 gcttctagct cttacgaatt aacacaacct ccatctgttt ctgtttctcc aggtcaaact
     3361 gctagaatta cttgttctgg tgatgctttg ccaaaacaat acacttactg gtatcaacaa
     3421 aaagctggtc aagctccagt tttggttatt tataaagata ctgaaagacc atctggtatt
     3481 ccagaaagat tctcaggtac ttcttctggt actactgcta ctttgactat ttctggtgtt
     3541 caagctgaag atgaagctga ttattattgt caatctgctg attcttctgg ttctcatgta
     3601 tttttcggtg gtggtactaa agttactgtt ttgtctggtg gaggaggccc aggtggcggt
     3661 tcaggtggag gttctggcgg aggttctggt ggaggttcct atgagttgac tcagccaccc
     3721 tcggtgtcag tgtccccagg acagacggcc aggatcacct gctctggaga tgcattgcca
     3781 aagcaatata cttattggta ccagcagaag gcaggccagg cccctgtctt ggtgatatat
     3841 aaagacactg agaggccctc agggatccct gagcgattct ctggtaccag ttcagggaca
     3901 acagccacat tgaccatcag tggagtccag gcagaagacg aggctgacta ttactgtcaa
     3961 tcagcagaca gcagtggttc ccacgttttc ttcggcggag ggaccaaggt gaccgtacta
     4021 tccggcaccg ccgcagctgg cgctggagca gcaggtggag cagctgcagg aatgctcatg
     4081 gcgtccacca cttccgctgt gcctgggcat ccctctctgc ccagcctgcc cagcaacagc
     4141 agccaggaga ggccactgga cacccgggac ccgctgctag cccgggcgga gctggcgctg
     4201 ctctccatag tctttgtggc tgtggccctg agcaatggcc tggtgctggc ggccctagct
     4261 cggcggggcc ggcggggcca ctgggcaccc atacacgtct tcattggcca cttgtgcctg
     4321 gccgacctgg ccgtggctct gttccaagtg ctgccccagc tggcctggaa ggccaccgac
     4381 cgcttccgtg ggccagatgc cctgtgtcgg gccgtgaagt atctgcagat ggtgggcatg
     4441 tatgcctcct cctacatgat cctggccatg acgctggacc gccaccgtgc catctgccgt
     4501 cccatgctgg cgtaccgcca tggaagtggg gctcactgga accggccggt gctagtggct
     4561 tgggccttct cgctccttct cagcctgccc cagctcttca tcttcgccca gcgcaacgtg
     4621 gaaggtggca gcggggtcac tgactgctgg gcctgctttg cggagccctg gggccgtcgc
     4681 acctatgtca cctggattgc cctgatggtg ttcgtggcac ctaccctggg tatcgccgcc
     4741 tgccaggtgc tcatcttccg ggagattcat gccagtctgg tgccagggcc atcagagagg
     4801 cctggggggc gccgcagggg acgccggaca ggcagccccg gtgagggagc ccacgtgtca
     4861 gcagctgtgg ccaagactgt gaggatgacg ctagtgattg tggtcgtcta tgtgctgtgc
     4921 tgggcaccct tcttcctggt gcagctgtgg gccgcgtggg acccggaggc acctctggaa
     4981 ggggcgccct ttgtgctact catgttgctg gccagcctca acagctgcac caacccctgg
     5041 atctatgcat ctttcagcag cagcgtgtcc tcagagctgc gaagcttgct ctgctgtgcc
     5101 cggggacgca ccccacccag cctgggtccc caagatgagt cctgcaccac cgccagctcc
     5161 tccctggcca aggacacttc atcgtgatgt acatagtaat gaacggatcc gtaattccgc
     5221 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     5281 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     5341 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     5401 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     5461 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     5521 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     5581 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     5641 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     5701 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5761 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga
     5821 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     5881 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     5941 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6001 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6061 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6121 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6181 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6241 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6301 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6361 gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag cccggtgcct gataggaatt
     6421 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6481 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6541 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6601 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6661 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6721 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6781 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6841 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6901 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6961 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7021 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     7081 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     7141 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     7201 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7261 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     7321 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     7381 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     7441 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     7501 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     7561 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7621 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7681 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     7741 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     7801 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     7861 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7921 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7981 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     8041 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8101 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8161 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8221 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8281 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8341 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8401 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8461 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     8521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8581 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8641 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8701 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     8761 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     8821 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     8881 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     8941 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     9001 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     9061 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     9121 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     9181 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     9241 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     9301 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     9361 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     9421 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9541 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     9601 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9661 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9721 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    10201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10681 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10741 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10801 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10861 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10921 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10981 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11041 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11101 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11161 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11221 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11281 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11341 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    11401 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11461 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11521 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11581 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11641 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11701 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
