LOCUS       sequence-148544-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  evolved sortase A (eSrtA) pentamutant with improved kinetics and 
            activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Chen I, Dorr BM, Liu DR
  TITLE     A general strategy for the evolution of bond-forming enzymes using 
            yeast display.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404. doi: 
            10.1073/pnas.1101046108. Epub 2011 Jun 22.
  PUBMED    21697512
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1101046108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-07-12- 12"; volume: "108"; issue: "28"; pages: 
            "11399-404"
COMMENT     Sequence Label: sortase A pentamutant (eSrtA) in pET29
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             506..528
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    543..567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(567..586)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(568..586)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtttgac
       61 ttctgtagct acaaagattt tacgtgtttc ccaaacgcct gtctcttcat tgtaatcatc
      121 acaagtaatt aatgttaatt gtttatcttt acctttttgt tcatctagaa cttctacagc
      181 tgttggctta acgtttctta tacttgtcat tttatactta cgtgtttcat taccaacttt
      241 aaagtacacc atactacctt ttttggctgc tttaagattt gtaaattgat agttcggacg
      301 gtcaatgaaa gtgtgtcctg caattgaaat attttgatca tctagtgatt cattttcttc
      361 tgcaaagctt acacctctat ttaattgttc gcgtgttgct ggtcctggat atactggttc
      421 tttaatatca gcatctggaa tttcaatata gcctgccact tttgatttat ctttcggaat
      481 ttgaggttta gcttgcatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga
      541 ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg
      601 agatcgatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg g
//
