LOCUS       sequence-148553-        9022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recruits VP64 to compatible gRNAs for transcriptional activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pLEX_307_ms2_VP64
FEATURES             Location/Qualifiers
     source          1..9022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1330..1350
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1360..1383
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1765..1914
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     protein_bind    complement(1931..1955)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1963..2004
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2055..2643
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2108..2128)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2526..2537)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2715..2948
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3026..3147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3063..3082)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3117..3136
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3187..3322
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3342..3361)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3343..3361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3371..3388)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3371..3387)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3380..3402)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3529..3984
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3616..3635)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3826..3847
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4010..4114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4115..4183,4184..4975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4333..4352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5146..5734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5635..5654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5888..5905
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6022..6043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6058..6063,6064..6081,6082..6088)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6096..6112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6101..6123
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6120..6136
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6120..6136
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6155..6175
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6157..6175
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6203..6429
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6430..6610
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6657..6782
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7275..7508
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7693..7737
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7968..8178
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7990..8186
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8037..8172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8218..8820
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8218..8237)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8714..8734
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8863..8980
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatgggac ctaagaaaaa gaggaaggtg gcggccgctg actacaagga tgacgacgat
     1381 aaatctagaa tggcttctaa ctttactcag ttcgttctcg tcgacaatgg cggaactggc
     1441 gacgtgactg tcgccccaag caacttcgct aacgggatcg ctgaatggat cagctctaac
     1501 tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc agagctctgc gcagaatcgc
     1561 aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc gttcgtactt aaatatggaa
     1621 ctaaccattc caattttcgc cacgaattcc gactgcgagc ttattgttaa ggcaatgcaa
     1681 ggtctcctaa aagatggaaa cccgattccc tcagcaatcg cagcaaactc cggcatctac
     1741 gaggccagcg gttccggacg ggctgacgca ttggacgatt ttgatctgga tatgctggga
     1801 agtgacgccc tcgatgattt tgaccttgac atgcttggtt cggatgccct tgatgacttt
     1861 gacctcgaca tgctcggcag tgacgccctt gatgatttcg acctggacat gctgattaac
     1921 tctagatgaa acccagcttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1981 cctctcctcg gtctcgattc tacgtagtaa tgaactagta ccggttaagt cgacaatcaa
     2041 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2101 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2161 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2221 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2281 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2341 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2401 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     2461 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2521 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2581 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     2641 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     2701 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     2761 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2821 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2881 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2941 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     3001 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     3061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3121 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     3181 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3241 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3301 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     3361 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3421 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     3481 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     3541 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3601 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3661 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3721 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3781 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3841 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3901 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3961 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4021 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4081 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4141 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4201 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4261 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4321 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4381 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4441 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4501 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4561 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4621 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4681 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4741 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4801 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4861 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4921 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4981 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5041 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5101 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5161 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5221 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5281 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5341 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5401 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5461 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5521 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5581 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5641 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5701 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5761 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5821 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5881 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     5941 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6001 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6061 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6121 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     6181 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     6241 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     6301 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     6361 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     6421 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6481 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6541 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6601 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     6661 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     6721 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     6781 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     6841 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     6901 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     6961 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     7021 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     7081 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     7141 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     7201 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     7261 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     7321 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     7381 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     7441 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     7501 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     7561 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     7621 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     7681 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     7741 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     7801 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     7861 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc catgcattgc
     7921 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7981 tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8041 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8101 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8161 tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga gcttaccatg
     8221 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     8281 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     8341 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     8401 ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac gccggagagc
     8461 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     8521 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     8581 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     8641 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     8701 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     8761 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa
     8821 cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     8881 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     8941 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     9001 cagagatcca ctttggcgcc gg
//
