LOCUS       sequence-148555-        9844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recruits p65_hsf1 to compatible gRNAs for transcriptional 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pLEX_307_ms2_p65_hsf1
FEATURES             Location/Qualifiers
     source          1..9844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1327..1713
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1768..1788
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1804..2346
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             2371..2742
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     protein_bind    complement(2753..2777)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2785..2826
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2877..3465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2930..2950)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3348..3359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3537..3770
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3848..3969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3885..3904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3939..3958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4009..4144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4164..4183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4165..4183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4193..4210)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4193..4209)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4202..4224)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4351..4806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4438..4457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4648..4669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4832..4936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4937..5005,5006..5797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5155..5174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5968..6556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6457..6476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6710..6727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6844..6865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6880..6885,6886..6903,6904..6910)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6918..6934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6923..6945
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6942..6958
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6942..6958
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6977..6997
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6979..6997
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7025..7251
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7252..7432
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7479..7604
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8097..8330
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8515..8559
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8790..9000
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8812..9008
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8859..8994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9040..9642
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9040..9059)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9536..9556
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9685..9802
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatggctt caaactttac tcagttcgtg ctcgtggaca atggtgggac aggggatgtg
     1381 acagtggctc cttctaattt cgctaatggg gtggcagagt ggatcagctc caactcacgg
     1441 agccaggcct acaaggtgac atgcagcgtc aggcagtcta gtgcccagaa gagaaagtat
     1501 accatcaagg tggaggtccc caaagtggct acccagacag tgggcggagt cgaactgcct
     1561 gtcgccgctt ggaggtccta cctgaacatg gagctcacta tcccaatttt cgctaccaat
     1621 tctgactgtg aactcatcgt gaaggcaatg caggggctcc tcaaagacgg taatcctatc
     1681 ccttccgcca tcgccgctaa ctcaggtatc tacagcgctg gaggaggtgg aagcggagga
     1741 ggaggaagcg gaggaggagg tagcggacct aagaaaaaga ggaaggtggc ggccgctgga
     1801 tccccttcag ggcagatcag caaccaggcc ctggctctgg cccctagctc cgctccagtg
     1861 ctggcccaga ctatggtgcc ctctagtgct atggtgcctc tggcccagcc acctgctcca
     1921 gcccctgtgc tgaccccagg accaccccag tcactgagcg ctccagtgcc caagtctaca
     1981 caggccggcg aggggactct gagtgaagct ctgctgcacc tgcagttcga cgctgatgag
     2041 gacctgggag ctctgctggg gaacagcacc gatcccggag tgttcacaga tctggcctcc
     2101 gtggacaact ctgagtttca gcagctgctg aatcagggcg tgtccatgtc tcatagtaca
     2161 gccgaaccaa tgctgatgga gtaccccgaa gccattaccc ggctggtgac cggcagccag
     2221 cggccccccg accccgctcc aactcccctg ggaaccagcg gcctgcctaa tgggctgtcc
     2281 ggagatgaag acttctcaag catcgctgat atggacttta gtgccctgct gtcacagatt
     2341 tcctctagtg ggcagggagg aggtggaagc ggcttcagcg tggacaccag tgccctgctg
     2401 gacctgttca gcccctcggt gaccgtgccc gacatgagcc tgcctgacct tgacagcagc
     2461 ctggccagta tccaagagct cctgtctccc caggagcccc ccaggcctcc cgaggcagag
     2521 aacagcagcc cggattcagg gaagcagctg gtgcactaca cagcgcagcc gctgttcctg
     2581 ctggaccccg gctccgtgga caccgggagc aacgacctgc cggtgctgtt tgagctggga
     2641 gagggctcct acttctccga aggggacggc ttcgccgagg accccaccat ctccctgctg
     2701 acaggctcgg agcctcccaa agccaaggac cccactgtct ccgctagctg aaacccagct
     2761 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     2821 tctacgtagt aatgaactag taccggttaa gtcgacaatc aacgcgttaa gtcgacaatc
     2881 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2941 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3001 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3061 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3121 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3181 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3241 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     3301 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3361 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3421 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     3481 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     3541 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     3601 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3661 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3721 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     3781 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     3841 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3961 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     4021 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4081 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4141 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     4201 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4261 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4321 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     4381 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4441 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4501 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4561 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4621 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4681 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4741 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4801 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4861 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4921 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4981 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5041 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5101 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5161 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5221 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5281 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5341 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5401 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5461 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5521 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5581 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5641 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5701 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5761 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5821 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5881 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5941 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6001 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6061 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6121 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6181 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6241 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6301 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6361 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6421 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6481 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6541 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6601 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6661 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6721 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     6961 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     7021 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     7081 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     7141 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     7201 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     7261 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7321 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7381 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     7441 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     7501 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     7561 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     7621 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     7681 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     7741 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     7801 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     7861 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     7921 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     7981 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8041 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     8101 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     8161 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     8221 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     8281 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     8341 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8401 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     8461 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8521 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8581 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8641 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8701 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     8761 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     8821 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8881 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8941 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9001 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     9061 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     9121 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     9181 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     9241 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     9301 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     9361 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     9421 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     9481 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     9541 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     9601 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     9661 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     9721 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9781 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     9841 ccgg
//
