LOCUS       sequence-148567-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac transposon encoding Neomycin resistance with deleted 
            inverted terminal repeat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Henssen AG, Henaff E, Jiang E, Eisenberg AR, Carson JR, Villasante 
            CM, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason CE, 
            Kentsis A
  TITLE     Genomic DNA transposition induced by human PGBD5.
  JOURNAL   Elife. 2015 Sep 25;4. pii: e10565. doi: 10.7554/eLife.10565.
  PUBMED    26406119
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-09-25"; volume: "4. pii: e10565. doi"; pages: " 
            10.7554/eLife.10565"
COMMENT     Sequence Label: PB-EF1-IRES-NEO deleted ITR
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(40..48,49..51,52..626)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(213..232)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     442..459
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tgcgtgcaat ccatcttgtt caatcattcc ggacgccatg gttgtggcca tattatcatc
       61 gtgtttttca aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc
      121 tcgactaaac acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg
      181 gtaccttctg ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc
      241 tttccacaac tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat
      301 cttatacacg tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc
      361 tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct
      421 tcacgacatt caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc
      481 tcgtcaagaa gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg
      541 tggaaaataa catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa
      601 cgttaggggg gggggaggga gaggggcgcg gccgcggatc cgatttaaat tcgaattctc
      661 gagctagctc tagagtaggc gccggtcaca gcttggatct gtaacggcgc agaacagaaa
      721 acgaaacaaa gacg
//
