LOCUS       sequence-148570-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Full length RCAN1 expression with CMV promoter.  Flag tagged..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Liu Q, Busby JC, Molkentin JD
  TITLE     Interaction between TAK1-TAB1-TAB2 and RCAN1-calcineurin defines a 
            signalling nodal control point.
  JOURNAL   Nat Cell Biol. 2009 Feb;11(2):154-61. doi: 10.1038/ncb1823. Epub 
            2009 Jan 11.
  PUBMED    19136967
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1823"; 
            journalName: "Nat Cell Biol"; date: "2009-02"; volume: "11"; issue: 
            "2"; pages: "154-61"
COMMENT     Sequence Label: RCAN1
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        248..266
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     248..265
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     279..298
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             341..364
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      987..996
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1037..1056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgcct
       61 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca
      121 ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc
      181 ccaccccctt ggcttcgtta gaacgcggct acaattaata cataacctta tgtatcatac
      241 acatacgatt taggtgacac tatagaataa catccacttt gcctttctct ccacaggtgt
      301 ccactcccag gtccaactgc acctcggttc tatcgatatg gactacaaag acgatgacga
      361 caagcgaatt ccccggggat ccatggagga ggtggatctg caggacctgc cgagcgccac
      421 catcgcctgc cacctggacc cgcgcgtgtt cgtggacggc ctgtgccggg ccaaatttga
      481 atccctcttc agaacatatg acaaggacac caccttccag tattttaaga gcttcaaacg
      541 tgtccggata aacttcagca accccttatc tgcagccgat gccaggctgc ggctgcacaa
      601 gaccgagttc ctggggaagg aaatgaagtt gtattttgct cagactttac acataggaag
      661 ttcacacctg gctccgccca atcccgacaa acagttcctc atctcccctc cggcctctcc
      721 tcccgttggc tggaaacaag tagaagatgc cacccccgtc ataaattacg atcttttata
      781 tgccatctcc aagctggggc caggagagaa gtatgaactg catgcagcga cagacaccac
      841 tcccagtgtg gtggtccacg tgtgtgagag tgaccaagag aatgaggagg aagaggaaga
      901 gatggagaga atgaagagac ccaagcccaa aatcatccag acacggagac cggagtacac
      961 accgatccac cttagctgaa agcttggccg ccatggccca acttgtttat tgcagcttat
     1021 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt ttt
//
