LOCUS       sequence-148572-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An adaption on the RCAS/tv-a somatic cell gene transfer system, for 
            use in combination with an existing Cas9 background in the 
            cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Fraser J, Simpson J, Fontana R, Kishi-Itakura C, Ktistakis NT, 
            Gammoh N
  TITLE     Targeting of early endosomes by autophagy facilitates EGFR recycling
            and signalling.
  JOURNAL   EMBO Rep. 2019 Oct 4;20(10):e47734. doi: 10.15252/embr.201947734. 
            Epub 2019 Aug 26.
  PUBMED    31448519
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2019-10-4"; volume: "20(10):e47734. doi"; pages: " 
            10.15252/embr.201947734. Epub 2019 Aug 26"
COMMENT     Sequence Label: RCAS-sgATG7
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(188..292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     360..378
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(421..426,427..444,445..451)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    459..475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     464..486
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
       61 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      301 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
      361 ataggcgtat cacgaggccc tttcgtcttc aagaattctc actcattagg caccccaggc
      421 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      481 cacaggaaac agccaagctt ggagctttaa tgcggtagtt tatcacagtt aaattgctaa
      541 cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc
      601 accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
      661 atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
      721 atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca
      781 gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc
      841 gtcctgtgga tcccctccga cggtactcag cttctgcctc cctaagccgc agccccctcc
      901 accagggtca tcgaactcgc ctcccgatag acgagacgga tggagacagg atcgccacgc
//
