LOCUS       sequence-148573-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, 
            Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array
            driven by its native leader, and the tmRNA::td intron construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Silas S, Mohr G, Sidote DJ, Markham LM, Sanchez-Amat A, Bhaya D, 
            Lambowitz AM, Fire AZ
  TITLE     Direct CRISPR spacer acquisition from RNA by a natural reverse 
            transcriptase-Cas1 fusion protein.
  JOURNAL   Science. 2016 Feb 26;351(6276):aad4234. doi: 
            10.1126/science.aad4234.
  PUBMED    26917774
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-02-26"; volume: "351(6276):aad4234. doi"; pages: " 
            10.1126/science.aad4234"
COMMENT     Sequence Label: pV92.2
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggcatggat gaactataca aataggtttg agctctaaac cctttatcag tgaataaacg
       61 atttttgctc tttaaaaaca taaccttaaa acagtcctca attgattgaa ggggtttagg
      121 gcgcgtttta cataaaaatc aaaaacttag cttgaaataa tggcgaaaat tcactaattt
      181 taagcatacc tcttgtggat aacttgaggg cgggggaaac gctaggttaa cctgctgaaa
      241 tgattggaaa aaataagggt actgtttcag acccgctggc cgcttaggcc gttgagactt
      301 taaagatctc cgccacgcaa accaattccc cgtttcagac ccgctggccg cttaggccgt
      361 tgagactggg ggttggggtt tctatttttc gttagtattc agagtttcag acccgctggc
      421 cgcttaggcc gttgagacaa gatattatct tcgttatggc ggaaattatc aatgtttcag
      481 acccgctggc cgcttaggcc gttgagactt actgaacgaa gactgggagg catgggagca
      541 gaatgtttca gacccgctgg ccgcttaggc cgttgagact ttggcggtat tgccatcatc
      601 tcctcatcaa gctgtttcag acccgctggc cgcttaggcc gttgagacaa gtaatacttg
      661 ctcaagtctt cagctatacg ataggtttca gacccgctgg ccgcttaggc cgttgagact
      721 tagatagaag ttgagaacac gtcagatgga ttaagtcgtt tcagacccgc tggccgctta
      781 ggccgttgag actttgacgc ataaacctgc gcgcacaagt gcgttctgtt tcagacccgc
      841 tggtcgttga gacgtgtgac gatgaaccat gactgttgga ttggaacggt tcggtttaac
      901 tggggg
//
