LOCUS       sequence-148604-        9056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium 
            acetobutylicum ATCC 824.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Li Q, Chen J, Minton NP, Zhang Y, Wen Z, Liu J, Yang H, Zeng Z, Ren 
            X, Yang J, Gu Y, Jiang W, Jiang Y, Yang S
  TITLE     CRISPR-based genome editing and expression control systems in 
            Clostridium acetobutylicum and Clostridium beijerinckii.
  JOURNAL   Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
  PUBMED    27213844
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2016 May 23. doi: 10.1002/biot.201600053."
COMMENT     Sequence Label: pdCASclos
FEATURES             Location/Qualifiers
     source          1..9056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..48
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        69..144
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(281..307,308..310,311..2797,2798..2800,2801..4387)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(4397..4414)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4397..4413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4406..4428)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4622..4641)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4741..4763
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4801..4819)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4887..4991
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4992..5060,5061..5852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5210..5229)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6023..6611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6512..6531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6765..6782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6899..6920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6935..6940,6941..6958,6959..6965)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6973..6989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6978..7000
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6997..7013
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6997..7013
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcttggctg cagttgacag ctagctcagt cctaggtata atactagttt taattgcaga
       61 tgataatagt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      121 gaaaaagtgg caccgagtcg gtgctttttt gcggccgcta taaaatataa ataattttct
      181 aaaaaactta acttcatgtg aaaagtttgt taaaatataa atgagcacgt taatcattta
      241 acatagataa ttaaatagta aaagggagtg tcgactcgag atggataaga aatactcaat
      301 aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt
      361 tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct
      421 tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac
      481 agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc
      541 aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt
      601 ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc
      661 ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga
      721 taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca
      781 ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca
      841 gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga
      901 tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc
      961 tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg
     1021 tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc
     1081 aaaagatact tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc
     1141 tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag
     1201 agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga
     1261 acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta
     1321 taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc
     1381 tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga
     1441 ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa
     1501 cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga
     1561 agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg
     1621 aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg
     1681 gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc
     1741 agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt
     1801 actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt
     1861 caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc
     1921 cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga
     1981 ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt
     2041 taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt
     2101 ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga
     2161 agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt
     2221 gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat
     2281 taatggtatt agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg
     2341 ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga
     2401 cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt
     2461 agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt
     2521 ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa
     2581 tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg
     2641 tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca
     2701 aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga
     2761 attagatatt aatcgtttaa gtgattatga tgtcgatgca attgttccac aaagtttcct
     2821 taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc
     2881 ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct
     2941 aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg
     3001 tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat
     3061 cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga
     3121 taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg
     3181 aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc
     3241 gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga
     3301 gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca
     3361 agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa
     3421 aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg
     3481 ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt
     3541 gtccatgccc caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa
     3601 ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga
     3661 tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc
     3721 taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac
     3781 aattatggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata
     3841 taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga
     3901 aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc
     3961 tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg
     4021 tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga
     4081 tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt
     4141 agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga
     4201 aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt
     4261 tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct
     4321 tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg
     4381 tgactgaccc gggggaactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     4441 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4501 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     4561 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     4621 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4681 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4741 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4801 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4861 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4921 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4981 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5041 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5101 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5161 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5221 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5281 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5341 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5401 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5461 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5521 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5581 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5641 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5701 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5761 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5821 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5881 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5941 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6001 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6061 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6901 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6961 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7021 acgccaagct ttggctaaca cacacgccat tccaaccaat agttttctcg gcataaagcc
     7081 atgctctgac gcttaaatgc actaatgcct taaaaaaaca ttaaagtcta acacactaga
     7141 cttatttact tcgtaattaa gtcgttaaac cgtgtgctct acgaccaaaa gtataaaacc
     7201 tttaagaact ttcttttttc ttgtaaaaaa agaaactaga taaatctctc atatctttta
     7261 ttcaataatc gcatcagatt gcagtataaa tttaacgatc actcatcatg ttcatattta
     7321 tcagagctcg tgctataatt atactaattt tataaggagg aaaaaataaa gagggttata
     7381 atgaacgaga aaaatataaa acacagtcaa aactttatta cttcaaaaca taatatagat
     7441 aaaataatga caaatataag attaaatgaa catgataata tctttgaaat cggctcagga
     7501 aaagggcatt ttacccttga attagtacag aggtgtaatt tcgtaactgc cattgaaata
     7561 gaccataaat tatgcaaaac tacagaaaat aaacttgttg atcacgataa tttccaagtt
     7621 ttaaacaagg atatattgca gtttaaattt cctaaaaacc aatcctataa aatatttggt
     7681 aatatacctt ataacataag tacggatata atacgcaaaa ttgtttttga tagtatagct
     7741 gatgagattt atttaatcgt ggaatacggg tttgctaaaa gattattaaa tacaaaacgc
     7801 tcattggcat tatttttaat ggcagaagtt gatatttcta tattaagtat ggttccaaga
     7861 gaatattttc atcctaaacc taaagtgaat agctcactta tcagattaaa tagaaaaaaa
     7921 tcaagaatat cacacaaaga taaacagaag tataattatt tcgttatgaa atgggttaac
     7981 aaagaataca agaaaatatt tacaaaaaat caatttaaca attccttaaa acatgcagga
     8041 attgacgatt taaacaatat tagctttgaa caattcttat ctcttttcaa tagctataaa
     8101 ttatttaata agtaagttaa gggatgcata aactgcatcc cttaacttgt ttttcgtgta
     8161 cctatttttt gtgaatcgat tatgtctttt gcgcattcac ttcttttcta tataaatatg
     8221 agcgaagcga ataagcgtcg gaaaagcagc aaaaagtttc ctttttgctg ttggagcatg
     8281 ggggttcagg gggtgcagta tctgacgtca atgccgagcg aaagcgagcc gaagggtagc
     8341 atttacgtta gataaccccc tgatatgctc cgacgcttta tatagaaaag aagattcaac
     8401 taggtaaaat cttaatatag gttgagatga taaggtttat aaggaatttg tttgttctaa
     8461 tttttcactc attttgttct aatttctttt aacaaatgtt cttttttttt tagaacagtt
     8521 atgatatagt tagaatagtt taaaataagg agtgagaaaa agatgaaaga aagatatgga
     8581 acagtctata aaggctctca gaggctcata gacgaagaaa gtggagaagt catagaggta
     8641 gacaagttat accgtaaaca aacgtctggt aacttcgtaa aggcatatat agtgcaatta
     8701 ataagtatgt tagatatgat tggcggaaaa aaacttaaaa tcgttaacta tatcctagat
     8761 aatgtccact taagtaacaa tacaatgata gctacaacaa gagaaatagc aaaagctaca
     8821 ggaacaagtc tacaaacagt aataacaaca cttaaaatct tagaagaagg aaatattata
     8881 aaaagaaaaa ctggagtatt aatgttaaac cctgaactac taatgagagg cgacgaccaa
     8941 aaacaaaaat acctcttact cgaatttggg aactttgagc aagaggcaaa tgaaatagat
     9001 tgacctccca ataacaccac gtagttattg ggaggtcaat ctatgaaatg cgatta
//
