LOCUS       sequence-148605-       15399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; p35S:Cas9, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 15399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE1
FEATURES             Location/Qualifiers
     source          1..15399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        318..420
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             421..1080
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(487..506)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1422..1727
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1606..1625
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    2069..2093
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3394..4023
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4457..5524
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5590..5784
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6020..6042)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6128..6268
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6183..6202
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6283..6300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6454..7042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6534..6553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7286..8077)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8605..8629
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8672..8924)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9314..10108)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9425..9444)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10035..10054
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(10129..10312)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(10165..10344)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(10437..10689)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(10705..10725)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(10738..14841)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(10738..14841)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(10738..14841)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(14932..15277)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(14969..14989)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagagagatc
       61 ttgaatgata gcctttcctt tatcgcaatg atggcatttg taggagccac cttccttttc
      121 tactgtcctt tcgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
      181 aattatcctt tgttgaaaag tctcaatagc cctttgatct tctgagactg tatctttgac
      241 atttttggag tagaccagag tgtcgtgctc caccatgttg acctccaagc ttgaattctt
      301 gcagagacct gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
      361 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
      421 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
      481 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      541 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      601 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
      661 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
      721 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
      781 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
      841 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
      901 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
      961 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat
     1021 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     1081 tcgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     1141 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
     1201 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
     1261 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
     1321 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
     1381 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca
     1441 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     1501 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
     1561 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     1621 atatggccag tgtgccggtt tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     1681 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
     1741 ttatacacag ccagtcggtc tcagcttcca tgctagagtc cgcaaaaatc accagtctct
     1801 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
     1861 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
     1921 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     1981 ccagtgacct gcaggcatgc gacgtcgagc ttagcttgag cttggatcag attgtcgttt
     2041 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     2101 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     2161 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     2221 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     2281 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     2341 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     2401 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     2461 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     2521 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     2581 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     2641 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     2701 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     2761 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     2821 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     2881 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     2941 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     3001 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     3061 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     3121 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     3181 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     3241 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     3301 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     3361 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     3421 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     3481 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     3541 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     3601 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     3661 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     3721 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     3781 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     3841 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     3901 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     3961 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     4021 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     4081 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     4141 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     4201 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     4261 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     4321 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     4381 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc
     4441 gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     4501 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     4561 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     4621 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     4681 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     4741 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     4801 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     4861 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     4921 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     4981 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     5041 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     5101 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     5161 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     5221 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     5281 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     5341 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     5401 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     5461 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     5521 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     5581 tgtctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     5641 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
     5701 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
     5761 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
     5821 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
     5881 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
     5941 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
     6001 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6061 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6121 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     6181 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6241 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     6301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7081 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat gatatatctc ccaatttgtg
     7141 tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg
     7201 tgagcaatta tgtgcttagt gcatctaatc gcttgagtta acgccggcga agcggcgtcg
     7261 gcttgaacga atttctagct agacattatt tgccgactac cttggtgatc tcgcctttca
     7321 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     7381 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     7441 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa
     7501 tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc
     7561 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga
     7621 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga
     7681 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct
     7741 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt
     7801 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag
     7861 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg
     7921 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
     7981 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
     8041 gttgagtcga tacttcggcg atcaccgctt cccccatgat gtttaacttt gttttagggc
     8101 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     8161 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa catgtcataa
     8221 caagaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     8281 gaccagttgc gtgacggcag ttacgctact tgcattacag cttacgaacc gaacgaggct
     8341 tatgtccact gggttcgtgc ccgaattgat cacaggcagc aacgctctgt catcgttaca
     8401 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     8461 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     8521 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     8581 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     8641 tagacaactt aataacacat tgcggaattc cgatctagta acatagatga caccgcgcgc
     8701 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
     8761 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
     8821 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
     8881 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggat catccgggtc
     8941 tgtggcggga actccacgaa aatatccgaa cgcagcaaga tatcgcggtg catctcggtc
     9001 ttgcctgggc agtcgccgcc gacgccgttg atgtggacgc cgggcccgat catattgtcg
     9061 ctcaggatcg tggcgttgtg cttgtcggcc gttgctgtcg taatgatatc ggcaccttcg
     9121 accgcctgtt ccgcagagat cccgtgggcg aagaactcca gcatgagatc cccgcgctgg
     9181 aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg
     9241 gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac
     9301 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     9361 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     9421 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     9481 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     9541 cgccatgggt cacgacgaga tcatcgccgt cgggcatgcg cgccttgagc ctggcgaaca
     9601 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     9661 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     9721 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     9781 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     9841 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     9901 gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct
     9961 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
    10021 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
    10081 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc agatccggtg cagattattt
    10141 ggattgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
    10201 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
    10261 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga
    10321 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
    10381 atcggcgggg gtcataacgt gactccctta attctccgct catgattagt agcgtcgatc
    10441 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
    10501 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
    10561 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
    10621 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
    10681 tgaacgatct gcttgacaag ctcacacctt cctcttcttc ttggggtcag ccctgctgtc
    10741 tccaccgagc tgagagaggt cgattcttgt ttcatagagc cccgtaattg actgatgaat
    10801 cagtgtggcg tccaggacct cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc
    10861 gaagtacttg aaggctgcag gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt
    10921 ttctgcctgc tccctgatgg gcttatccct gtgcttattg taagcagaaa gcaccttatc
    10981 gaggttagcg tcggcgagga tcactctttt ggagaattcg cttatttgct cgatgatctc
    11041 atcaaggtag tgtttgtgtt gttccacgaa cagctgcttc tgctcattat cttcgggaga
    11101 tcctttgagc ttttcatagt ggctggccag atacaagaaa ttaacgtatt tagagggcag
    11161 tgccagctcg ttacctttct gcagctcgcc cgcactagcg agcattcgtt tccggccgtt
    11221 ttcaagctca aagagagagt acttgggaag cttaatgatg aggtcttttt tgacctcttt
    11281 atatcctttc gcctcgagaa agtcgatggg gtttttttcg aagcttgatc gctccatgat
    11341 tgtgatgccc agcagttcct tgacgctttt gagtttttta gacttccctt tctccacttt
    11401 ggccacaacc agtacactgt aagcgactgt aggagaatcg aatccgccgt atttcttggg
    11461 gtcccaatct tttttgcgtg cgatcagctt gtcgctgttc cttttcggga ggatactttc
    11521 cttggagaag cctccggtct gtacttcggt ctttttaacg atgttcacct gcggcatgga
    11581 caggaccttc cggactgtcg cgaaatccct acccttgtcc cacacgattt ctcctgtttc
    11641 tccgtttgtt tcgataagtg gtcgcttccg aatctctcca ttggccagtg taatctcggt
    11701 cttgaaaaaa ttcataatat tgctgtaaaa gaagtactta gcggtggcct tgcctatttc
    11761 ctgctcagac tttgcgatca ttttcctaac atcgtacact ttatagtctc cgtaaacaaa
    11821 ttcagattca agcttgggat attttttgat aagtgcagtg cctaccactg cattcaggta
    11881 ggcatcatgc gcatggtggt aattgttgat ctctctcacc ttataaaact gaaagtcctt
    11941 tctgaaatct gaaaccagct tagacttcag agtaataact ttcacctctc gaatcagttt
    12001 gtcattttca tcgtacttgg tgttcatgcg tgaatcgaga atttgggcca cgtgcttggt
    12061 gatctggcgt gtctcaacaa gctgcctttt gatgaagccg gctttatcca actcagacag
    12121 gccacctcgt tcagccttag tcagattatc gaacttccgt tgtgtgatca gtttggcgtt
    12181 cagcagctgc cgccaataat ttttcatttt cttgacaact tcttctgagg ggacgttatc
    12241 actcttccct ctatttttat cggatcttgt caacacttta ttatcaatag aatcatcttt
    12301 gagaaaagac tggggcacga tatgatccac gtcgtagtcg gagagccgat tgatgtccag
    12361 ttcctgatcc acgtacatgt ccctgccgtt ctgcaggtag tacaggtaga gcttctcatt
    12421 ctgaagctgg gtgttttcaa ctgggtgttc cttaaggatt tgggacccca gttcttttat
    12481 accctcttca atcctcttca tcctttccct actgttcttc tgtcccttct gggtagtttg
    12541 gttctctcgg gccatctcga taacgatatt ctcgggctta tgccttccca ttactttgac
    12601 gagttcatcc acgaccttaa cggtctgcag tattcccttt ttgatagctg ggctacctgc
    12661 aagattagcg atgtgctcgt ggagactgtc cccctggcca gaaacttgtg ctttctggat
    12721 gtcctcctta aaggtgagag agtcatcatg gatcaactgc atgaagttcc ggttggcaaa
    12781 tccatcggac ttaagaaaat ccaggattgt ctttccactc tgcttgtctc ggatcccatt
    12841 gatcagtttt cttgacagcc gcccccatcc tgtatatcgg cgcctcttga gctgtttcat
    12901 gactttgtcg tcgaagagat gagcgtaagt tttcaagcgt tcttcaatca tctccctatc
    12961 ttcaaacaac gtaagggtga ggacaatgtc ctcaagaatg tcctcgttct cctcattgtc
    13021 caggaagtcc ttgtctttaa tgattttcag gagatcgtga tacgttccca gggatgcgtt
    13081 gaagcgatcc tccactccgc tgatttcaac agagtcgaaa cattcaatct ttttgaaata
    13141 atcttctttg agctgtttca cggtaacttt ccggttcgtc ttgaagagga ggtccacgat
    13201 agctttcttc tgctctccag acaggaatgc tggctttctc atcccttctg tgacgtattt
    13261 gaccttggtg agctcgttat aaactgtgaa gtactcgtac agcagagagt gtttaggaag
    13321 caccttttcg ttaggcagat ttttatcaaa gttagtcatc ctttcgatga aggactgggc
    13381 agaggccccc ttatccacga cttcctcgaa gttccaggga gtgatagtct cttctgattt
    13441 gcgagtcatc cacgcgaatc tggaatttcc ccgggcgagg gggcctacat agtagggtat
    13501 ccgaaatgtg aggattttct caatcttttc cctgttatct ttcaaaaagg ggtagaaatc
    13561 ctcttgccgc ctgaggatag cgtgcagttc gcccaggtga atctggtggg ggatgcttcc
    13621 attgtcgaaa gtgcgctgtt tgcgcaacag atcttctctg ttaagcttta ccagcagctc
    13681 ctcggtgccg tccatttttt ccaagatggg cttaataaat ttgtaaaatt cctcctggct
    13741 tgctccgccg tcaatgtatc cggcgtagcc atttttagac tgatcgaaga aaatttcctt
    13801 gtacttctca ggcagttgct gtctgacaag ggccttcagc aaagtcaagt cttggtggtg
    13861 ctcatcatag cgcttgatca tactagcgct cagcggagct ttggtgatct ccgtgttcac
    13921 tcgcagaata tcactcagca gaatggcgtc tgacaggttc tttgccgcca aaaaaaggtc
    13981 tgcgtactgg tcgccgatct gggccagcag attgtcgaga tcatcatcgt aggtgtcttt
    14041 gctcagttga agcttggcat cttcggccag gtcgaagtta gatttaaagt tgggggtcag
    14101 cccgagtgac agggcgataa gattaccaaa caggccgttc ttcttctccc cagggagctg
    14161 tgcgatgagg ttttcgagcc gccgggattt ggacagccta gcgctcagga ttgctttggc
    14221 gtcaactccg gatgcgttga tcgggttctc ttcgaaaagc tgattgtaag tctgaaccag
    14281 ttggataaag agtttgtcga catcgctgtt gtctgggttc aggtccccct cgatgaggaa
    14341 gtgtccccga aatttgatca tatgcgccag cgcgagatag atcaaccgca agtcagcctt
    14401 atcagtactg tctacaagct tcttcctcag atgatatatg gttgggtact tttcatggta
    14461 cgccacctcg tccacgatat tgccaaagat tgggtggcgc tcgtgctttt tatcctcctc
    14521 caccaaaaag gactcctcca gcctatggaa gaaagagtca tccaccttag ccatctcatt
    14581 actaaagatc tcctgcaggt agcagatccg attctttctg cgggtatatc tgcgccgtgc
    14641 tgttcttttg agccgcgtgg cttcggccgt ctccccggag tcgaacagga gggcgccaat
    14701 gaggttcttc tttatgctgt ggcgatcggt attgcccaga actttgaatt ttttgctcgg
    14761 caccttgtac tcgtccgtaa tgacggccca gccgacgctg tttgtgccga tatcgagccc
    14821 aatggagtac ttcttgtcca ttgtatcgat aattgtaaat gtaattgtaa tgttgtttgt
    14881 tgtttgttgt tgttggtaat tgttgtaaaa atgagctctt atactcgagc gtgtcctctc
    14941 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
    15001 gtcatccctt acgtcagtgg agatgtcaca tcaatccact tgctttgtag acgtggttgg
    15061 aacctcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
    15121 ggcagagaga tcttgaatga tagcctttcc tttatcgcaa tgatggcatt tgtaggagcc
    15181 accttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat ggaatccgag
    15241 gaggtttccc gaaattatcc tttgttgaaa agtctcaata gccctttgat cttctgagac
    15301 tgtatctttg acatttttgg agtagaccag agtgtcgtgc tccaccatgt tatcacatca
    15361 atccacttgc tttgtagacg tggttggaac ctcttcttt
//
