LOCUS       sequence-148606-       14772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; p35S:Cas9, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14772)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 14772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE2
FEATURES             Location/Qualifiers
     source          1..14772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    266..290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(333..585)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(605..1156)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1199..1378)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(1471..1723)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1739..1759)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1772..5875)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(1772..5875)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(1772..5875)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(5966..6311)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6003..6023)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        6751..6853
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6854..7513
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6920..6939)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7855..8160
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8039..8058
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    8502..8526
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9827..10456
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10890..11957
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      12023..12217
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12453..12475)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12561..12701
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12616..12635
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12716..12733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12887..13475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12967..12986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13719..14510)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
       61 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
      121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
      181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
      241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
      301 ttagacaact taataacaca ttgcggaatt ccgatctagt aacatagatg acaccgcgcg
      361 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
      421 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
      481 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
      541 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctaggg
      601 gtcatcagat ttcggtgacg ggcaggaccg gacggggcgg caccggcagg ctgaagtcca
      661 gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc
      721 cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga
      781 tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga
      841 gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg
      901 tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc
      961 cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc
     1021 actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt
     1081 tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc
     1141 gtcgttctgg gctcatggta gatccccctc gatcgagttg agagtgaata tgagactcta
     1201 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg
     1261 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg
     1321 cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg
     1381 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc
     1441 cttaattctc cgctcatgat tagtagcgtc gatctagtaa catagatgac accgcgcgcg
     1501 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt
     1561 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta
     1621 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag
     1681 gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttga caagctcaca
     1741 ccttcctctt cttcttgggg tcagccctgc tgtctccacc gagctgagag aggtcgattc
     1801 ttgtttcata gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg
     1861 tagaggtgta ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc
     1921 ccaagttggt cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat
     1981 ccctgtgctt attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc
     2041 ttttggagaa ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca
     2101 cgaacagctg cttctgctca ttatcttcgg gagatccttt gagcttttca tagtggctgg
     2161 ccagatacaa gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct
     2221 cgcccgcact agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg
     2281 gaagcttaat gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga
     2341 tggggttttt ttcgaagctt gatcgctcca tgattgtgat gcccagcagt tccttgacgc
     2401 ttttgagttt tttagacttc cctttctcca ctttggccac aaccagtaca ctgtaagcga
     2461 ctgtaggaga atcgaatccg ccgtatttct tggggtccca atcttttttg cgtgcgatca
     2521 gcttgtcgct gttccttttc gggaggatac tttccttgga gaagcctccg gtctgtactt
     2581 cggtcttttt aacgatgttc acctgcggca tggacaggac cttccggact gtcgcgaaat
     2641 ccctaccctt gtcccacacg atttctcctg tttctccgtt tgtttcgata agtggtcgct
     2701 tccgaatctc tccattggcc agtgtaatct cggtcttgaa aaaattcata atattgctgt
     2761 aaaagaagta cttagcggtg gccttgccta tttcctgctc agactttgcg atcattttcc
     2821 taacatcgta cactttatag tctccgtaaa caaattcaga ttcaagcttg ggatattttt
     2881 tgataagtgc agtgcctacc actgcattca ggtaggcatc atgcgcatgg tggtaattgt
     2941 tgatctctct caccttataa aactgaaagt cctttctgaa atctgaaacc agcttagact
     3001 tcagagtaat aactttcacc tctcgaatca gtttgtcatt ttcatcgtac ttggtgttca
     3061 tgcgtgaatc gagaatttgg gccacgtgct tggtgatctg gcgtgtctca acaagctgcc
     3121 ttttgatgaa gccggcttta tccaactcag acaggccacc tcgttcagcc ttagtcagat
     3181 tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa taatttttca
     3241 ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt ttatcggatc
     3301 ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc acgatatgat
     3361 ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac atgtccctgc
     3421 cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt tcaactgggt
     3481 gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc ttcatccttt
     3541 ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc tcgataacga
     3601 tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc ttaacggtct
     3661 gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc tcgtggagac
     3721 tgtccccctg gccagaaact tgtgctttct ggatgtcctc cttaaaggtg agagagtcat
     3781 catggatcaa ctgcatgaag ttccggttgg caaatccatc ggacttaaga aaatccagga
     3841 ttgtctttcc actctgcttg tctcggatcc cattgatcag ttttcttgac agccgccccc
     3901 atcctgtata tcggcgcctc ttgagctgtt tcatgacttt gtcgtcgaag agatgagcgt
     3961 aagttttcaa gcgttcttca atcatctccc tatcttcaaa caacgtaagg gtgaggacaa
     4021 tgtcctcaag aatgtcctcg ttctcctcat tgtccaggaa gtccttgtct ttaatgattt
     4081 tcaggagatc gtgatacgtt cccagggatg cgttgaagcg atcctccact ccgctgattt
     4141 caacagagtc gaaacattca atctttttga aataatcttc tttgagctgt ttcacggtaa
     4201 ctttccggtt cgtcttgaag aggaggtcca cgatagcttt cttctgctct ccagacagga
     4261 atgctggctt tctcatccct tctgtgacgt atttgacctt ggtgagctcg ttataaactg
     4321 tgaagtactc gtacagcaga gagtgtttag gaagcacctt ttcgttaggc agatttttat
     4381 caaagttagt catcctttcg atgaaggact gggcagaggc ccccttatcc acgacttcct
     4441 cgaagttcca gggagtgata gtctcttctg atttgcgagt catccacgcg aatctggaat
     4501 ttccccgggc gagggggcct acatagtagg gtatccgaaa tgtgaggatt ttctcaatct
     4561 tttccctgtt atctttcaaa aaggggtaga aatcctcttg ccgcctgagg atagcgtgca
     4621 gttcgcccag gtgaatctgg tgggggatgc ttccattgtc gaaagtgcgc tgtttgcgca
     4681 acagatcttc tctgttaagc tttaccagca gctcctcggt gccgtccatt ttttccaaga
     4741 tgggcttaat aaatttgtaa aattcctcct ggcttgctcc gccgtcaatg tatccggcgt
     4801 agccattttt agactgatcg aagaaaattt ccttgtactt ctcaggcagt tgctgtctga
     4861 caagggcctt cagcaaagtc aagtcttggt ggtgctcatc atagcgcttg atcatactag
     4921 cgctcagcgg agctttggtg atctccgtgt tcactcgcag aatatcactc agcagaatgg
     4981 cgtctgacag gttctttgcc gccaaaaaaa ggtctgcgta ctggtcgccg atctgggcca
     5041 gcagattgtc gagatcatca tcgtaggtgt ctttgctcag ttgaagcttg gcatcttcgg
     5101 ccaggtcgaa gttagattta aagttggggg tcagcccgag tgacagggcg ataagattac
     5161 caaacaggcc gttcttcttc tccccaggga gctgtgcgat gaggttttcg agccgccggg
     5221 atttggacag cctagcgctc aggattgctt tggcgtcaac tccggatgcg ttgatcgggt
     5281 tctcttcgaa aagctgattg taagtctgaa ccagttggat aaagagtttg tcgacatcgc
     5341 tgttgtctgg gttcaggtcc ccctcgatga ggaagtgtcc ccgaaatttg atcatatgcg
     5401 ccagcgcgag atagatcaac cgcaagtcag ccttatcagt actgtctaca agcttcttcc
     5461 tcagatgata tatggttggg tacttttcat ggtacgccac ctcgtccacg atattgccaa
     5521 agattgggtg gcgctcgtgc tttttatcct cctccaccaa aaaggactcc tccagcctat
     5581 ggaagaaaga gtcatccacc ttagccatct cattactaaa gatctcctgc aggtagcaga
     5641 tccgattctt tctgcgggta tatctgcgcc gtgctgttct tttgagccgc gtggcttcgg
     5701 ccgtctcccc ggagtcgaac aggagggcgc caatgaggtt cttctttatg ctgtggcgat
     5761 cggtattgcc cagaactttg aattttttgc tcggcacctt gtactcgtcc gtaatgacgg
     5821 cccagccgac gctgtttgtg ccgatatcga gcccaatgga gtacttcttg tccattgtat
     5881 cgataattgt aaatgtaatt gtaatgttgt ttgttgtttg ttgttgttgg taattgttgt
     5941 aaaaatgagc tcttatactc gagcgtgtcc tctccaaatg aaatgaactt ccttatatag
     6001 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatgt
     6061 cacatcaatc cacttgcttt gtagacgtgg ttggaacctc ttctttttcc acgatgctcc
     6121 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga gagatcttga atgatagcct
     6181 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttctact gtcctttcga
     6241 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgaaatt atcctttgtt
     6301 gaaaagtctc aatagccctt tgatcttctg agactgtatc tttgacattt ttggagtaga
     6361 ccagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgta gacgtggttg
     6421 gaacctcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     6481 cggcagagag atcttgaatg atagcctttc ctttatcgca atgatggcat ttgtaggagc
     6541 caccttcctt ttctactgtc ctttcgatga agtgacagat agctgggcaa tggaatccga
     6601 ggaggtttcc cgaaattatc ctttgttgaa aagtctcaat agccctttga tcttctgaga
     6661 ctgtatcttt gacatttttg gagtagacca gagtgtcgtg ctccaccatg ttgacctcca
     6721 agcttgaatt cttgcagaga cctgagacgt tgatcggcac gtaagaggtt ccaactttca
     6781 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
     6841 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     6901 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     6961 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     7021 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     7081 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     7141 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     7201 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     7261 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     7321 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     7381 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg
     7441 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     7501 gggcggggcg taatcgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt
     7561 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
     7621 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
     7681 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
     7741 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
     7801 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag
     7861 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     7921 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
     7981 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     8041 atgatgacca ccgatatggc cagtgtgccg gtttccgtta tcggggaaga agtggctgat
     8101 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     8161 atgtcaggct cccttataca cagccagtcg gtctcagctt ccatgctaga gtccgcaaaa
     8221 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     8281 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     8341 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     8401 ctaaaaccaa aatccagtga cctgcaggca tgcgacgtcg agcttagctt gagcttggat
     8461 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     8521 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     8581 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     8641 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     8701 ccgtgttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     8761 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     8821 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     8881 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     8941 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     9001 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     9061 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     9121 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     9181 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     9241 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     9301 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     9361 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     9421 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     9481 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     9541 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     9601 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     9661 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     9721 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     9781 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     9841 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     9901 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     9961 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
    10021 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
    10081 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
    10141 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
    10201 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
    10261 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
    10321 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
    10381 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
    10441 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
    10501 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
    10561 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
    10621 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
    10681 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
    10741 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
    10801 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
    10861 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
    10921 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
    10981 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
    11041 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
    11101 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
    11161 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
    11221 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
    11281 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
    11341 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
    11401 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
    11461 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
    11521 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
    11581 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
    11641 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
    11701 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
    11761 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
    11821 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
    11881 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
    11941 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
    12001 aaggtcgaaa aggtgtcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
    12061 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
    12121 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
    12181 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
    12241 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
    12301 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
    12361 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
    12421 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    12481 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    12541 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
    12601 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
    12661 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
    12721 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    12781 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    12841 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    12901 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    12961 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    13021 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    13081 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    13141 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    13201 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    13261 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    13321 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    13381 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    13441 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    13501 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
    13561 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
    13621 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
    13681 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
    13741 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
    13801 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
    13861 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
    13921 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
    13981 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
    14041 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
    14101 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
    14161 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
    14221 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
    14281 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
    14341 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
    14401 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
    14461 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
    14521 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
    14581 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
    14641 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
    14701 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
    14761 accgaacgag gc
//
