LOCUS       sequence-148609-       15579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  recipient plasmid [single nuclease]; p35S:Cas9, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15579)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 15579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE62
FEATURES             Location/Qualifiers
     source          1..15579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    266..290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(333..585)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(975..1769)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1086..1105)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1696..1715
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1790..1973)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(1826..2005)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(2098..2350)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2366..2386)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2399..6502)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2399..6502)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2399..6502)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(6593..6938)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6630..6650)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        7469..7571
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7572..8231
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7638..7657)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8577..8882
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8761..8780
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        8920..8995
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9309..9333
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10634..11263
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11697..12764
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      12830..13024
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13260..13282)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13368..13508
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13423..13442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13523..13540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13694..14282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13774..13793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14526..15317)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
       61 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
      121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
      181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
      241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
      301 ttagacaact taataacaca ttgcggaatt ccgatctagt aacatagatg acaccgcgcg
      361 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
      421 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
      481 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
      541 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga tcatccgggt
      601 ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt
      661 cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgggcccga tcatattgtc
      721 gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc gtaatgatat cggcaccttc
      781 gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc agcatgagat ccccgcgctg
      841 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
      901 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
      961 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1021 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1081 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1141 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1201 tcgccatggg tcacgacgag atcatcgccg tcgggcatgc gcgccttgag cctggcgaac
     1261 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1321 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1381 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1441 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1501 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1561 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1621 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     1681 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     1741 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc cagatccggt gcagattatt
     1801 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     1861 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     1921 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg
     1981 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     2041 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgattag tagcgtcgat
     2101 ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt
     2161 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
     2221 ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata
     2281 tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt
     2341 ttgaacgatc tgcttgacaa gctcacacct tcctcttctt cttggggtca gccctgctgt
     2401 ctccaccgag ctgagagagg tcgattcttg tttcatagag ccccgtaatt gactgatgaa
     2461 tcagtgtggc gtccaggacc tcctttgtag aggtgtaccg ctttctgtct atggtggtgt
     2521 cgaagtactt gaaggctgca ggcgcgccca agttggtcag agtaaacaag tggataatgt
     2581 tttctgcctg ctccctgatg ggcttatccc tgtgcttatt gtaagcagaa agcaccttat
     2641 cgaggttagc gtcggcgagg atcactcttt tggagaattc gcttatttgc tcgatgatct
     2701 catcaaggta gtgtttgtgt tgttccacga acagctgctt ctgctcatta tcttcgggag
     2761 atcctttgag cttttcatag tggctggcca gatacaagaa attaacgtat ttagagggca
     2821 gtgccagctc gttacctttc tgcagctcgc ccgcactagc gagcattcgt ttccggccgt
     2881 tttcaagctc aaagagagag tacttgggaa gcttaatgat gaggtctttt ttgacctctt
     2941 tatatccttt cgcctcgaga aagtcgatgg ggtttttttc gaagcttgat cgctccatga
     3001 ttgtgatgcc cagcagttcc ttgacgcttt tgagtttttt agacttccct ttctccactt
     3061 tggccacaac cagtacactg taagcgactg taggagaatc gaatccgccg tatttcttgg
     3121 ggtcccaatc ttttttgcgt gcgatcagct tgtcgctgtt ccttttcggg aggatacttt
     3181 ccttggagaa gcctccggtc tgtacttcgg tctttttaac gatgttcacc tgcggcatgg
     3241 acaggacctt ccggactgtc gcgaaatccc tacccttgtc ccacacgatt tctcctgttt
     3301 ctccgtttgt ttcgataagt ggtcgcttcc gaatctctcc attggccagt gtaatctcgg
     3361 tcttgaaaaa attcataata ttgctgtaaa agaagtactt agcggtggcc ttgcctattt
     3421 cctgctcaga ctttgcgatc attttcctaa catcgtacac tttatagtct ccgtaaacaa
     3481 attcagattc aagcttggga tattttttga taagtgcagt gcctaccact gcattcaggt
     3541 aggcatcatg cgcatggtgg taattgttga tctctctcac cttataaaac tgaaagtcct
     3601 ttctgaaatc tgaaaccagc ttagacttca gagtaataac tttcacctct cgaatcagtt
     3661 tgtcattttc atcgtacttg gtgttcatgc gtgaatcgag aatttgggcc acgtgcttgg
     3721 tgatctggcg tgtctcaaca agctgccttt tgatgaagcc ggctttatcc aactcagaca
     3781 ggccacctcg ttcagcctta gtcagattat cgaacttccg ttgtgtgatc agtttggcgt
     3841 tcagcagctg ccgccaataa tttttcattt tcttgacaac ttcttctgag gggacgttat
     3901 cactcttccc tctattttta tcggatcttg tcaacacttt attatcaata gaatcatctt
     3961 tgagaaaaga ctggggcacg atatgatcca cgtcgtagtc ggagagccga ttgatgtcca
     4021 gttcctgatc cacgtacatg tccctgccgt tctgcaggta gtacaggtag agcttctcat
     4081 tctgaagctg ggtgttttca actgggtgtt ccttaaggat ttgggacccc agttctttta
     4141 taccctcttc aatcctcttc atcctttccc tactgttctt ctgtcccttc tgggtagttt
     4201 ggttctctcg ggccatctcg ataacgatat tctcgggctt atgccttccc attactttga
     4261 cgagttcatc cacgacctta acggtctgca gtattccctt tttgatagct gggctacctg
     4321 caagattagc gatgtgctcg tggagactgt ccccctggcc agaaacttgt gctttctgga
     4381 tgtcctcctt aaaggtgaga gagtcatcat ggatcaactg catgaagttc cggttggcaa
     4441 atccatcgga cttaagaaaa tccaggattg tctttccact ctgcttgtct cggatcccat
     4501 tgatcagttt tcttgacagc cgcccccatc ctgtatatcg gcgcctcttg agctgtttca
     4561 tgactttgtc gtcgaagaga tgagcgtaag ttttcaagcg ttcttcaatc atctccctat
     4621 cttcaaacaa cgtaagggtg aggacaatgt cctcaagaat gtcctcgttc tcctcattgt
     4681 ccaggaagtc cttgtcttta atgattttca ggagatcgtg atacgttccc agggatgcgt
     4741 tgaagcgatc ctccactccg ctgatttcaa cagagtcgaa acattcaatc tttttgaaat
     4801 aatcttcttt gagctgtttc acggtaactt tccggttcgt cttgaagagg aggtccacga
     4861 tagctttctt ctgctctcca gacaggaatg ctggctttct catcccttct gtgacgtatt
     4921 tgaccttggt gagctcgtta taaactgtga agtactcgta cagcagagag tgtttaggaa
     4981 gcaccttttc gttaggcaga tttttatcaa agttagtcat cctttcgatg aaggactggg
     5041 cagaggcccc cttatccacg acttcctcga agttccaggg agtgatagtc tcttctgatt
     5101 tgcgagtcat ccacgcgaat ctggaatttc cccgggcgag ggggcctaca tagtagggta
     5161 tccgaaatgt gaggattttc tcaatctttt ccctgttatc tttcaaaaag gggtagaaat
     5221 cctcttgccg cctgaggata gcgtgcagtt cgcccaggtg aatctggtgg gggatgcttc
     5281 cattgtcgaa agtgcgctgt ttgcgcaaca gatcttctct gttaagcttt accagcagct
     5341 cctcggtgcc gtccattttt tccaagatgg gcttaataaa tttgtaaaat tcctcctggc
     5401 ttgctccgcc gtcaatgtat ccggcgtagc catttttaga ctgatcgaag aaaatttcct
     5461 tgtacttctc aggcagttgc tgtctgacaa gggccttcag caaagtcaag tcttggtggt
     5521 gctcatcata gcgcttgatc atactagcgc tcagcggagc tttggtgatc tccgtgttca
     5581 ctcgcagaat atcactcagc agaatggcgt ctgacaggtt ctttgccgcc aaaaaaaggt
     5641 ctgcgtactg gtcgccgatc tgggccagca gattgtcgag atcatcatcg taggtgtctt
     5701 tgctcagttg aagcttggca tcttcggcca ggtcgaagtt agatttaaag ttgggggtca
     5761 gcccgagtga cagggcgata agattaccaa acaggccgtt cttcttctcc ccagggagct
     5821 gtgcgatgag gttttcgagc cgccgggatt tggacagcct agcgctcagg attgctttgg
     5881 cgtcaactcc ggatgcgttg atcgggttct cttcgaaaag ctgattgtaa gtctgaacca
     5941 gttggataaa gagtttgtcg acatcgctgt tgtctgggtt caggtccccc tcgatgagga
     6001 agtgtccccg aaatttgatc atatgcgcca gcgcgagata gatcaaccgc aagtcagcct
     6061 tatcagtact gtctacaagc ttcttcctca gatgatatat ggttgggtac ttttcatggt
     6121 acgccacctc gtccacgata ttgccaaaga ttgggtggcg ctcgtgcttt ttatcctcct
     6181 ccaccaaaaa ggactcctcc agcctatgga agaaagagtc atccacctta gccatctcat
     6241 tactaaagat ctcctgcagg tagcagatcc gattctttct gcgggtatat ctgcgccgtg
     6301 ctgttctttt gagccgcgtg gcttcggccg tctccccgga gtcgaacagg agggcgccaa
     6361 tgaggttctt ctttatgctg tggcgatcgg tattgcccag aactttgaat tttttgctcg
     6421 gcaccttgta ctcgtccgta atgacggccc agccgacgct gtttgtgccg atatcgagcc
     6481 caatggagta cttcttgtcc attgtatcga taattgtaaa tgtaattgta atgttgtttg
     6541 ttgtttgttg ttgttggtaa ttgttgtaaa aatgagctct tatactcgag cgtgtcctct
     6601 ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg
     6661 cgtcatccct tacgtcagtg gagatgtcac atcaatccac ttgctttgta gacgtggttg
     6721 gaacctcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     6781 cggcagagag atcttgaatg atagcctttc ctttatcgca atgatggcat ttgtaggagc
     6841 caccttcctt ttctactgtc ctttcgatga agtgacagat agctgggcaa tggaatccga
     6901 ggaggtttcc cgaaattatc ctttgttgaa aagtctcaat agccctttga tcttctgaga
     6961 ctgtatcttt gacatttttg gagtagacca gagtgtcgtg ctccaccatg ttatcacatc
     7021 aatccacttg ctttgtagac gtggttggaa cctcttcttt ttccacgatg ctcctcgtgg
     7081 gtgggggtcc atctttggga ccactgtcgg cagagagatc ttgaatgata gcctttcctt
     7141 tatcgcaatg atggcatttg taggagccac cttccttttc tactgtcctt tcgatgaagt
     7201 gacagatagc tgggcaatgg aatccgagga ggtttcccga aattatcctt tgttgaaaag
     7261 tctcaatagc cctttgatct tctgagactg tatctttgac atttttggag tagaccagag
     7321 tgtcgtgctc caccatgttg acctccaagc ttgaattctt gctaagttga aaacaatctt
     7381 caaaagtccc acatcgctta gataagaaaa cgaagctgag tttatataca gctagagtcg
     7441 aagtagtgat tgaagtcttc tgagacgttg atcggcacgt aagaggttcc aactttcacc
     7501 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     7561 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     7621 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     7681 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     7741 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     7801 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     7861 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     7921 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     7981 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     8041 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     8101 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg
     8161 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     8221 ggggggcgta aacgctcgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     8281 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     8341 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     8401 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     8461 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     8521 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     8581 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     8641 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     8701 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     8761 gcatgatgac caccgatatg gccagtgtgc cggtttccgt tatcggggaa gaagtggctg
     8821 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     8881 aaatgtcagg ctcccttata cacagccagt cgaagacttg ttttagagct agaaatagca
     8941 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     9001 tttgcttcca tgctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct
     9061 ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata
     9121 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     9181 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgacct gcaggcatgc
     9241 gacgtcgagc ttagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     9301 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     9361 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     9421 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     9481 gcagtcggct tctgacgttc agtgcagccg tgttctgaaa acgacatgtc gcacaagtcc
     9541 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     9601 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     9661 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     9721 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     9781 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     9841 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     9901 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     9961 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
    10021 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
    10081 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
    10141 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
    10201 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
    10261 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
    10321 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
    10381 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
    10441 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
    10501 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
    10561 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
    10621 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
    10681 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
    10741 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
    10801 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
    10861 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
    10921 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
    10981 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
    11041 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
    11101 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
    11161 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
    11221 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
    11281 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
    11341 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
    11401 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
    11461 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
    11521 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
    11581 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
    11641 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc
    11701 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
    11761 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
    11821 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
    11881 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
    11941 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
    12001 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
    12061 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
    12121 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
    12181 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
    12241 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
    12301 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
    12361 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
    12421 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
    12481 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
    12541 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
    12601 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
    12661 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
    12721 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
    12781 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tgtctttcct gtggatagca
    12841 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
    12901 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
    12961 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
    13021 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
    13081 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
    13141 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
    13201 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
    13261 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    13321 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
    13381 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
    13441 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    13501 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    13561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    13621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    13681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    13741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    13801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    13861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    13921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    13981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    14041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    14101 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    14161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    14221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    14281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    14341 aagggatttt ggtcatgcat gatatatctc ccaatttgtg tagggcttat tatgcacgct
    14401 taaaaataat aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt
    14461 gcatctaatc gcttgagtta acgccggcga agcggcgtcg gcttgaacga atttctagct
    14521 agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
    14581 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
    14641 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
    14701 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
    14761 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
    14821 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
    14881 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
    14941 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
    15001 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
    15061 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
    15121 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
    15181 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
    15241 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
    15301 atcaccgctt cccccatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
    15361 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
    15421 gcccgaggca tagactgtac cccaaaaaaa catgtcataa caagaagcca tgaaaaccgc
    15481 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgacggcag
    15541 ttacgctact tgcattacag cttacgaacc gaacgaggc
//
