LOCUS       sequence-148611-       16159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16159)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 16159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE64
FEATURES             Location/Qualifiers
     source          1..16159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    266..290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(333..585)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(975..1769)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1086..1105)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1696..1715
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1790..1973)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(1826..2005)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(2099..2806)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(2817..2837)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2850..6953)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2850..6953)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2850..6953)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        8049..8151
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8152..8811
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8218..8237)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9157..9462
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9341..9360
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        9500..9575
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9889..9913
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11214..11843
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12277..13344
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      13410..13604
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13840..13862)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13948..14088
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14003..14022
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14103..14120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14274..14862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14354..14373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15106..15897)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
       61 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
      121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
      181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
      241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
      301 ttagacaact taataacaca ttgcggaatt ccgatctagt aacatagatg acaccgcgcg
      361 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
      421 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
      481 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
      541 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga tcatccgggt
      601 ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt
      661 cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgggcccga tcatattgtc
      721 gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc gtaatgatat cggcaccttc
      781 gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc agcatgagat ccccgcgctg
      841 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
      901 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
      961 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1021 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1081 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1141 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1201 tcgccatggg tcacgacgag atcatcgccg tcgggcatgc gcgccttgag cctggcgaac
     1261 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1321 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1381 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1441 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1501 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1561 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1621 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     1681 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     1741 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc cagatccggt gcagattatt
     1801 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     1861 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     1921 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg
     1981 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     2041 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgattag tagcggtcct
     2101 gctgagcctc gacatgttgt cgcaaaattc gccctggacc cgcccaacga tttgtcgtca
     2161 ctgtcaaggt ttgacctgca cttcatttgg ggcccacata caccaaaaaa atgctgcata
     2221 attctcgggg cagcaagtcg gttacccggc cgccgtgctg gaccgggttg aatggtgccc
     2281 gtaactttcg gtagagcgga cggccaatac tcaacttcaa ggaatctcac ccatgcgcgc
     2341 cggcggggaa ccggagttcc cttcagtgag cgttattagt tcgccgctcg gtgtgtcgta
     2401 gatactagcc cctggggcac ttttgaaatt tgaataagat ttatgtaatc agtcttttag
     2461 gtttgaccgg ttctgccgct ttttttaaaa ttggatttgt aataataaaa cgcaattgtt
     2521 tgttattgtg gcgctctatc atagatgtcg ctataaacct attcagcaca atatattgtt
     2581 ttcattttaa tattgtacat ataagtagta gggtacaatc agtaaattga acggagaata
     2641 ttattcataa aaatacgata gtaacgggtg atatattcat tagaatgaac cgaaaccggc
     2701 ggtaaggatc tgagctacac atgctcaggt tttttacaac gtgcacaaca gaattgaaag
     2761 caaatatcat gcgatcatag gcttctcgca tatctcatta aagcaggaca agctcacacc
     2821 ttcctcttct tcttggggtc agccctgctg tctccaccga gctgagagag gtcgattctt
     2881 gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac ctcctttgta
     2941 gaggtgtacc gctttctgtc tatggtggtg tcgaagtact tgaaggctgc aggcgcgccc
     3001 aagttggtca gagtaaacaa gtggataatg ttttctgcct gctccctgat gggcttatcc
     3061 ctgtgcttat tgtaagcaga aagcacctta tcgaggttag cgtcggcgag gatcactctt
     3121 ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg ttgttccacg
     3181 aacagctgct tctgctcatt atcttcggga gatcctttga gcttttcata gtggctggcc
     3241 agatacaaga aattaacgta tttagagggc agtgccagct cgttaccttt ctgcagctcg
     3301 cccgcactag cgagcattcg tttccggccg ttttcaagct caaagagaga gtacttggga
     3361 agcttaatga tgaggtcttt tttgacctct ttatatcctt tcgcctcgag aaagtcgatg
     3421 gggttttttt cgaagcttga tcgctccatg attgtgatgc ccagcagttc cttgacgctt
     3481 ttgagttttt tagacttccc tttctccact ttggccacaa ccagtacact gtaagcgact
     3541 gtaggagaat cgaatccgcc gtatttcttg gggtcccaat cttttttgcg tgcgatcagc
     3601 ttgtcgctgt tccttttcgg gaggatactt tccttggaga agcctccggt ctgtacttcg
     3661 gtctttttaa cgatgttcac ctgcggcatg gacaggacct tccggactgt cgcgaaatcc
     3721 ctacccttgt cccacacgat ttctcctgtt tctccgtttg tttcgataag tggtcgcttc
     3781 cgaatctctc cattggccag tgtaatctcg gtcttgaaaa aattcataat attgctgtaa
     3841 aagaagtact tagcggtggc cttgcctatt tcctgctcag actttgcgat cattttccta
     3901 acatcgtaca ctttatagtc tccgtaaaca aattcagatt caagcttggg atattttttg
     3961 ataagtgcag tgcctaccac tgcattcagg taggcatcat gcgcatggtg gtaattgttg
     4021 atctctctca ccttataaaa ctgaaagtcc tttctgaaat ctgaaaccag cttagacttc
     4081 agagtaataa ctttcacctc tcgaatcagt ttgtcatttt catcgtactt ggtgttcatg
     4141 cgtgaatcga gaatttgggc cacgtgcttg gtgatctggc gtgtctcaac aagctgcctt
     4201 ttgatgaagc cggctttatc caactcagac aggccacctc gttcagcctt agtcagatta
     4261 tcgaacttcc gttgtgtgat cagtttggcg ttcagcagct gccgccaata atttttcatt
     4321 ttcttgacaa cttcttctga ggggacgtta tcactcttcc ctctattttt atcggatctt
     4381 gtcaacactt tattatcaat agaatcatct ttgagaaaag actggggcac gatatgatcc
     4441 acgtcgtagt cggagagccg attgatgtcc agttcctgat ccacgtacat gtccctgccg
     4501 ttctgcaggt agtacaggta gagcttctca ttctgaagct gggtgttttc aactgggtgt
     4561 tccttaagga tttgggaccc cagttctttt ataccctctt caatcctctt catcctttcc
     4621 ctactgttct tctgtccctt ctgggtagtt tggttctctc gggccatctc gataacgata
     4681 ttctcgggct tatgccttcc cattactttg acgagttcat ccacgacctt aacggtctgc
     4741 agtattccct ttttgatagc tgggctacct gcaagattag cgatgtgctc gtggagactg
     4801 tccccctggc cagaaacttg tgctttctgg atgtcctcct taaaggtgag agagtcatca
     4861 tggatcaact gcatgaagtt ccggttggca aatccatcgg acttaagaaa atccaggatt
     4921 gtctttccac tctgcttgtc tcggatccca ttgatcagtt ttcttgacag ccgcccccat
     4981 cctgtatatc ggcgcctctt gagctgtttc atgactttgt cgtcgaagag atgagcgtaa
     5041 gttttcaagc gttcttcaat catctcccta tcttcaaaca acgtaagggt gaggacaatg
     5101 tcctcaagaa tgtcctcgtt ctcctcattg tccaggaagt ccttgtcttt aatgattttc
     5161 aggagatcgt gatacgttcc cagggatgcg ttgaagcgat cctccactcc gctgatttca
     5221 acagagtcga aacattcaat ctttttgaaa taatcttctt tgagctgttt cacggtaact
     5281 ttccggttcg tcttgaagag gaggtccacg atagctttct tctgctctcc agacaggaat
     5341 gctggctttc tcatcccttc tgtgacgtat ttgaccttgg tgagctcgtt ataaactgtg
     5401 aagtactcgt acagcagaga gtgtttagga agcacctttt cgttaggcag atttttatca
     5461 aagttagtca tcctttcgat gaaggactgg gcagaggccc ccttatccac gacttcctcg
     5521 aagttccagg gagtgatagt ctcttctgat ttgcgagtca tccacgcgaa tctggaattt
     5581 ccccgggcga gggggcctac atagtagggt atccgaaatg tgaggatttt ctcaatcttt
     5641 tccctgttat ctttcaaaaa ggggtagaaa tcctcttgcc gcctgaggat agcgtgcagt
     5701 tcgcccaggt gaatctggtg ggggatgctt ccattgtcga aagtgcgctg tttgcgcaac
     5761 agatcttctc tgttaagctt taccagcagc tcctcggtgc cgtccatttt ttccaagatg
     5821 ggcttaataa atttgtaaaa ttcctcctgg cttgctccgc cgtcaatgta tccggcgtag
     5881 ccatttttag actgatcgaa gaaaatttcc ttgtacttct caggcagttg ctgtctgaca
     5941 agggccttca gcaaagtcaa gtcttggtgg tgctcatcat agcgcttgat catactagcg
     6001 ctcagcggag ctttggtgat ctccgtgttc actcgcagaa tatcactcag cagaatggcg
     6061 tctgacaggt tctttgccgc caaaaaaagg tctgcgtact ggtcgccgat ctgggccagc
     6121 agattgtcga gatcatcatc gtaggtgtct ttgctcagtt gaagcttggc atcttcggcc
     6181 aggtcgaagt tagatttaaa gttgggggtc agcccgagtg acagggcgat aagattacca
     6241 aacaggccgt tcttcttctc cccagggagc tgtgcgatga ggttttcgag ccgccgggat
     6301 ttggacagcc tagcgctcag gattgctttg gcgtcaactc cggatgcgtt gatcgggttc
     6361 tcttcgaaaa gctgattgta agtctgaacc agttggataa agagtttgtc gacatcgctg
     6421 ttgtctgggt tcaggtcccc ctcgatgagg aagtgtcccc gaaatttgat catatgcgcc
     6481 agcgcgagat agatcaaccg caagtcagcc ttatcagtac tgtctacaag cttcttcctc
     6541 agatgatata tggttgggta cttttcatgg tacgccacct cgtccacgat attgccaaag
     6601 attgggtggc gctcgtgctt tttatcctcc tccaccaaaa aggactcctc cagcctatgg
     6661 aagaaagagt catccacctt agccatctca ttactaaaga tctcctgcag gtagcagatc
     6721 cgattctttc tgcgggtata tctgcgccgt gctgttcttt tgagccgcgt ggcttcggcc
     6781 gtctccccgg agtcgaacag gagggcgcca atgaggttct tctttatgct gtggcgatcg
     6841 gtattgccca gaactttgaa ttttttgctc ggcaccttgt actcgtccgt aatgacggcc
     6901 cagccgacgc tgtttgtgcc gatatcgagc ccaatggagt acttcttgtc cattgctgca
     6961 catacataac atatcaagat cagaacacac atatacacac acaaatacaa tcaagtcaac
     7021 aactccaaaa agtccagatc tacatatata catacgtaaa taacaaaatc atgtaaataa
     7081 tcacaatcat gtaatccaga tctatgcaca tatatatata cacaattaat aaaaaaaatg
     7141 atataacaga tctatatcta tgtatgtaac aacacaatca gatgagagaa gtgatgtttt
     7201 cagatctgta tacatacaaa cacaaacaga tgaacaattg atacgtagat ccatatgtat
     7261 acgtacaatt agctacacga ttaaatgaaa aaaatcaacg atttcggatt ggtacacaca
     7321 aacgcaacaa tatgaagaaa ttcatatctg attagatata aacataacca cgtgtagata
     7381 cacagtcaaa tcaacaaatt tatagcttct aaacggatga gatgaacaag ataaagatat
     7441 tcacataagg catacataag ataagcagat taacaaacta gcaataatac atacctaatt
     7501 aaaacaagga ataacagaga gagagagaga gagagagatt taccttgaaa atgaagagga
     7561 gaagagagga tttcttaaaa ttgggggtag agaaagaaag atgatgaatt gtgagaaagg
     7621 agagatagaa gggggggttg tatatatagg ctgtagaaga ttatttttgt gtttgaggcg
     7681 gtgaaggaag aggggatctg actatgacac gtttgcggtt acgtatttcg ataggagtct
     7741 ttcaacgctt aacgccgtta ctctatatga ccgtttgggc cgtaacgggg ccgtttgtta
     7801 acgctgatgt tgattctttt ctttctttct ttcttccttt tttaaagaag caattgtaca
     7861 atcgttgcta gctgtcaaac ggataattcg gatacggata tgcctatatt catatccgta
     7921 atctccaagc ttgaattctt gctaagttga aaacaatctt caaaagtccc acatcgctta
     7981 gataagaaaa cgaagctgag tttatataca gctagagtcg aagtagtgat tgaagtcttc
     8041 tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     8101 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
     8161 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     8221 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     8281 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     8341 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     8401 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     8461 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     8521 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     8581 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     8641 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     8701 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga
     8761 atgcttaatg aattacaaca gtactgcgat gagtggcagg ggggggcgta aacgctcgcg
     8821 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     8881 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     8941 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     9001 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     9061 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     9121 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     9181 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     9241 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     9301 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     9361 gccagtgtgc cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     9421 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     9481 cacagccagt cgaagacttg ttttagagct agaaatagca agttaaaata aggctagtcc
     9541 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttgcttcca tgctagagtc
     9601 cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat
     9661 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     9721 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     9781 ctaattccta aaaccaaaat ccagtgacct gcaggcatgc gacgtcgagc ttagcttgag
     9841 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     9901 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     9961 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
    10021 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
    10081 agtgcagccg tgttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
    10141 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
    10201 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
    10261 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
    10321 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
    10381 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
    10441 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
    10501 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
    10561 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
    10621 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
    10681 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
    10741 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
    10801 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
    10861 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
    10921 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
    10981 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
    11041 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
    11101 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
    11161 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
    11221 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
    11281 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
    11341 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
    11401 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
    11461 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
    11521 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
    11581 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
    11641 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
    11701 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
    11761 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
    11821 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
    11881 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
    11941 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
    12001 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
    12061 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
    12121 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
    12181 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
    12241 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta
    12301 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
    12361 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
    12421 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
    12481 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
    12541 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
    12601 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
    12661 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
    12721 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
    12781 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
    12841 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
    12901 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
    12961 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
    13021 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
    13081 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
    13141 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
    13201 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
    13261 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
    13321 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
    13381 ggggaaaaag gtcgaaaagg tgtctttcct gtggatagca cgtacattgg gaacccaaag
    13441 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
    13501 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
    13561 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
    13621 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
    13681 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
    13741 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
    13801 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    13861 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    13921 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    13981 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    14041 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
    14101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    14161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    14221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    14281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    14341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    14401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    14461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    14521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    14581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    14641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    14701 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    14761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    14821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    14881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
    14941 gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac
    15001 ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaatc gcttgagtta
    15061 acgccggcga agcggcgtcg gcttgaacga atttctagct agacattatt tgccgactac
    15121 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
    15181 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
    15241 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
    15301 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
    15361 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
    15421 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
    15481 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
    15541 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
    15601 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
    15661 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
    15721 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
    15781 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
    15841 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt cccccatgat
    15901 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
    15961 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
    16021 cccaaaaaaa catgtcataa caagaagcca tgaaaaccgc cactgcgccg ttaccaccgc
    16081 tgcgttcggt caaggttctg gaccagttgc gtgacggcag ttacgctact tgcattacag
    16141 cttacgaacc gaacgaggc
//
