LOCUS       sequence-148616-       17192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 17192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE151
FEATURES             Location/Qualifiers
     source          1..17192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2125..3192
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3258..3452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3688..3710)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3796..3936
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3851..3870
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3951..3968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4122..4710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4202..4221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4954..5745)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6266..6290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6434..6612
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6466..6648
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      7248..7500
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7986..8681)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(9888..10595)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(10606..10626)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(10639..14742)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(10639..14742)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(10639..14742)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        15369..15471
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             15472..16131
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(15538..15557)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             16473..16778
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     16657..16676
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    16899..16923
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg
     2161 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     2221 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     2281 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     2341 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     2401 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     2461 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     2521 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     2581 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     2641 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     2701 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     2761 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     2821 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     2881 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     2941 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     3001 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     3061 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     3121 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     3181 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     3241 cgaaaaggtg tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     3301 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     3361 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     3421 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     3481 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     3541 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     3601 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     3661 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     3721 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     3781 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     3841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3901 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     3961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4561 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc
     4801 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     4861 tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac gccggcgaag
     4921 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc
     4981 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     5041 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     5101 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     5161 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     5221 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     5281 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     5341 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     5401 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     5461 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     5521 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     5581 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     5641 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     5701 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt
     5761 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     5821 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     5881 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     5941 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
     6001 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
     6061 aatcagcatg ctaccctccg cgagatcatc cgtgtttaaa acccggcagc ttagttgccg
     6121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     6181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     6241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     6301 ttagacaact taataacaca ttgcggatcc ctcggtcaca tgtgcatcct ctctgggagc
     6361 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
     6421 tttggaactg acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt
     6481 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca
     6541 ctatcagcta gcaaatattt cttgtcaaaa tgctccactg acgttccata aattcccctc
     6601 ggtatccaat tagagtctca tattcactct caatccaaat aatctgcacc ggatctggat
     6661 cgtttcgcat gtctcctgaa agaaggcctg ctgatattag aagggctact gaggctgata
     6721 tgcctgctgt gtgcactatt gtgaatcatt acattgagac ttctactgtg aatttcagga
     6781 ctgagcctca agaaccacaa gagtggactg atgatcttgt gaggcttaga gagaggtatc
     6841 cttggcttgt tgctgaagtg gatggtgaag tggctggtat tgcttatgct ggtccttgga
     6901 aggctaggaa tgcttacgat tggactgctg agtctactgt ttacgtgtct cctaggcatc
     6961 aaagaactgg tcttggatct actctttaca ctcatctttt gaagtctttg gaggctcagg
     7021 gtttcaagtc tgtggttgct gtgattggac ttcctaatga tccttctgtg aggatgcatg
     7081 aggctcttgg ttatgctcct aggggaatgc ttagagctgc tggtttcaaa catggaaatt
     7141 ggcatgatgt gggattctgg cagcttgatt tctctcttcc tgttcctcct agacctgttc
     7201 ttcctgtgac tgagatttga gcgggactct ggatctagag tcaagcagat cgttcaaaca
     7261 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     7321 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     7381 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     7441 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     7501 gacgcttgct gaattggagc gctccatggg aattcgtaaa gcgactaaat ggagcaacct
     7561 actgtttttg aagtttgggt tgccatgtaa tcacttgcaa taaatatatg tactaatgac
     7621 tgcatcggct tgcgtgtcta aatttgtagc ttattgaaaa ctttttgtgg ttaaagacga
     7681 atccagttaa ctaacataag tttaacaaaa caagaccttt tccatatata gaccaccgtt
     7741 accaacttac aaagaagcca aataaagtac aacacaacac ttgcaagtta caaaaagaga
     7801 ggaaaaaaaa catggtacca tttgacaaca tatagaaaca cacataaaca ttacttataa
     7861 caaaaagatt caaactcggc ttttattatc tccccctgat caaattctaa acactttatt
     7921 cctcataaac taactccccg acattggagt tattggacta tgacgatgac atcagtgggg
     7981 taagctcact tgtgccccag tttgctaggg aggtcgcagt atctggccac agccacctcg
     8041 tgctgctcga cgtaggtttc tttgtcggcc tccttgattc tttccagtct gtggtccaca
     8101 tagtagacgc cgggcatctt gaggttctta gcgggtttct tggatctgta tgtggtcttg
     8161 aagttgcaga tcaggtggcc cccgcccacg agcttcaggg ccatgtcgct tctgccttcc
     8221 aggccgccgt cagcggggta cagcatctcg gtgttggcct cccagccgag tgttttcttc
     8281 tgcatcacag ggccgttgga tgggaagttc acccctctga tcttgacgtt gtagatgagg
     8341 cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccatc ttcgtatgtg
     8401 gtgactctct cccatgtgaa gccctcaggg aaggactgct taaagaagtc ggggatgccc
     8461 tgggtgtggt tgatgaaggt tctgctgccg tacatgaagc tggtagccag gatgtcgaag
     8521 gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt gccctcgtag
     8581 ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt tgttcacggt gccctccatg
     8641 tacagcttca tgtgcatgtt ctccttaatc agctcgctca tagtagtgtg ctggccacca
     8701 cgagtacggt cacggtcatg ttccccacca gtatgttgct gtccgtagta ctgagctctg
     8761 tctttcagat cctgagcttt gcttcccaac ttcatccttg cactgtccaa cttgtctgat
     8821 ccctgtgggt gctctcccgt tgcgtaccta ttcacaatca ataggtatca taccaagcat
     8881 tttacctata tagatgatat acacagacac attcaagcta gctaggtcaa atttcgtatt
     8941 tagttaacca aatgttccct agaatttgca atttcttatt gttacatttg ttattcgacc
     9001 aaaaaaattg atccaaagct actggctcaa cacatgcatg cacatattgc acaaaattat
     9061 gaagtaagat gataaatgtg tgcttacttg taaatccaag agaaaacggt tatagcggca
     9121 atgccaaacc ctccagagga aagaaaaccg gtgatgagga gtgcaactgt gatgagagcc
     9181 gggacaagga ttgggctgaa gataacgagc agaggtgttg caacagtcaa agctatgaca
     9241 gttccaacaa gggtaaggct ggagagaaca aggagggaac caccagctgt gacagcagtt
     9301 gcagctttag caatctgcct agacttggag tagtcagatc ctcgtccgga catctggtac
     9361 tggtcacgat ctcggcccat catcgggtac tgatctctgc cgatgatatc gtgatgggtt
     9421 cctctagctg tatccgccat ttttttgttc ttgtttacta gagagaatga gattttgaat
     9481 gagagattga tcaaaaagat gtgtatatat tttggaagag gtaggagtgt ttgttagata
     9541 tattaagaaa ggagacgaga agtcacgtgt caatggagac atcatgcaat tgtaggccac
     9601 gtgttaatgg cgtcaccatg cagccgctgc atgtgttaac gagaggagtc tattcaaaga
     9661 cacgtttgtt ttttgcctag ctcagaatgt ggcgttggca tcgaaccgaa acataaaacg
     9721 caaaacgaac ctttgggctc gtcagtatgg gtttgggcct tcaatagatt ccttctatac
     9781 ggcccacata tccactattg gcaacaaaat tagaaaaatt taggatttag ttttaaaatt
     9841 tgttccgcga cattctagaa ctccgagctc gaattctagt agcggtcctg ctgagcctcg
     9901 acatgttgtc gcaaaattcg ccctggaccc gcccaacgat ttgtcgtcac tgtcaaggtt
     9961 tgacctgcac ttcatttggg gcccacatac accaaaaaaa tgctgcataa ttctcggggc
    10021 agcaagtcgg ttacccggcc gccgtgctgg accgggttga atggtgcccg taactttcgg
    10081 tagagcggac ggccaatact caacttcaag gaatctcacc catgcgcgcc ggcggggaac
    10141 cggagttccc ttcagtgagc gttattagtt cgccgctcgg tgtgtcgtag atactagccc
    10201 ctggggcact tttgaaattt gaataagatt tatgtaatca gtcttttagg tttgaccggt
    10261 tctgccgctt tttttaaaat tggatttgta ataataaaac gcaattgttt gttattgtgg
    10321 cgctctatca tagatgtcgc tataaaccta ttcagcacaa tatattgttt tcattttaat
    10381 attgtacata taagtagtag ggtacaatca gtaaattgaa cggagaatat tattcataaa
    10441 aatacgatag taacgggtga tatattcatt agaatgaacc gaaaccggcg gtaaggatct
    10501 gagctacaca tgctcaggtt ttttacaacg tgcacaacag aattgaaagc aaatatcatg
    10561 cgatcatagg cttctcgcat atctcattaa agcaggacaa gctcacacct tcctcttctt
    10621 cttggggtca gccctgctgt ctccaccgag ctgagagagg tcgattcttg tttcatagag
    10681 ccccgtaatt gactgatgaa tcagtgtggc gtccaggacc tcctttgtag aggtgtaccg
    10741 ctttctgtct atggtggtgt cgaagtactt gaaggctgca ggcgcgccca agttggtcag
    10801 agtaaacaag tggataatgt tttctgcctg ctccctgatg ggcttatccc tgtgcttatt
    10861 gtaagcagaa agcaccttat cgaggttagc gtcggcgagg atcactcttt tggagaattc
    10921 gcttatttgc tcgatgatct catcaaggta gtgtttgtgt tgttccacga acagctgctt
    10981 ctgctcatta tcttcgggag atcctttgag cttttcatag tggctggcca gatacaagaa
    11041 attaacgtat ttagagggca gtgccagctc gttacctttc tgcagctcgc ccgcactagc
    11101 gagcattcgt ttccggccgt tttcaagctc aaagagagag tacttgggaa gcttaatgat
    11161 gaggtctttt ttgacctctt tatatccttt cgcctcgaga aagtcgatgg ggtttttttc
    11221 gaagcttgat cgctccatga ttgtgatgcc cagcagttcc ttgacgcttt tgagtttttt
    11281 agacttccct ttctccactt tggccacaac cagtacactg taagcgactg taggagaatc
    11341 gaatccgccg tatttcttgg ggtcccaatc ttttttgcgt gcgatcagct tgtcgctgtt
    11401 ccttttcggg aggatacttt ccttggagaa gcctccggtc tgtacttcgg tctttttaac
    11461 gatgttcacc tgcggcatgg acaggacctt ccggactgtc gcgaaatccc tacccttgtc
    11521 ccacacgatt tctcctgttt ctccgtttgt ttcgataagt ggtcgcttcc gaatctctcc
    11581 attggccagt gtaatctcgg tcttgaaaaa attcataata ttgctgtaaa agaagtactt
    11641 agcggtggcc ttgcctattt cctgctcaga ctttgcgatc attttcctaa catcgtacac
    11701 tttatagtct ccgtaaacaa attcagattc aagcttggga tattttttga taagtgcagt
    11761 gcctaccact gcattcaggt aggcatcatg cgcatggtgg taattgttga tctctctcac
    11821 cttataaaac tgaaagtcct ttctgaaatc tgaaaccagc ttagacttca gagtaataac
    11881 tttcacctct cgaatcagtt tgtcattttc atcgtacttg gtgttcatgc gtgaatcgag
    11941 aatttgggcc acgtgcttgg tgatctggcg tgtctcaaca agctgccttt tgatgaagcc
    12001 ggctttatcc aactcagaca ggccacctcg ttcagcctta gtcagattat cgaacttccg
    12061 ttgtgtgatc agtttggcgt tcagcagctg ccgccaataa tttttcattt tcttgacaac
    12121 ttcttctgag gggacgttat cactcttccc tctattttta tcggatcttg tcaacacttt
    12181 attatcaata gaatcatctt tgagaaaaga ctggggcacg atatgatcca cgtcgtagtc
    12241 ggagagccga ttgatgtcca gttcctgatc cacgtacatg tccctgccgt tctgcaggta
    12301 gtacaggtag agcttctcat tctgaagctg ggtgttttca actgggtgtt ccttaaggat
    12361 ttgggacccc agttctttta taccctcttc aatcctcttc atcctttccc tactgttctt
    12421 ctgtcccttc tgggtagttt ggttctctcg ggccatctcg ataacgatat tctcgggctt
    12481 atgccttccc attactttga cgagttcatc cacgacctta acggtctgca gtattccctt
    12541 tttgatagct gggctacctg caagattagc gatgtgctcg tggagactgt ccccctggcc
    12601 agaaacttgt gctttctgga tgtcctcctt aaaggtgaga gagtcatcat ggatcaactg
    12661 catgaagttc cggttggcaa atccatcgga cttaagaaaa tccaggattg tctttccact
    12721 ctgcttgtct cggatcccat tgatcagttt tcttgacagc cgcccccatc ctgtatatcg
    12781 gcgcctcttg agctgtttca tgactttgtc gtcgaagaga tgagcgtaag ttttcaagcg
    12841 ttcttcaatc atctccctat cttcaaacaa cgtaagggtg aggacaatgt cctcaagaat
    12901 gtcctcgttc tcctcattgt ccaggaagtc cttgtcttta atgattttca ggagatcgtg
    12961 atacgttccc agggatgcgt tgaagcgatc ctccactccg ctgatttcaa cagagtcgaa
    13021 acattcaatc tttttgaaat aatcttcttt gagctgtttc acggtaactt tccggttcgt
    13081 cttgaagagg aggtccacga tagctttctt ctgctctcca gacaggaatg ctggctttct
    13141 catcccttct gtgacgtatt tgaccttggt gagctcgtta taaactgtga agtactcgta
    13201 cagcagagag tgtttaggaa gcaccttttc gttaggcaga tttttatcaa agttagtcat
    13261 cctttcgatg aaggactggg cagaggcccc cttatccacg acttcctcga agttccaggg
    13321 agtgatagtc tcttctgatt tgcgagtcat ccacgcgaat ctggaatttc cccgggcgag
    13381 ggggcctaca tagtagggta tccgaaatgt gaggattttc tcaatctttt ccctgttatc
    13441 tttcaaaaag gggtagaaat cctcttgccg cctgaggata gcgtgcagtt cgcccaggtg
    13501 aatctggtgg gggatgcttc cattgtcgaa agtgcgctgt ttgcgcaaca gatcttctct
    13561 gttaagcttt accagcagct cctcggtgcc gtccattttt tccaagatgg gcttaataaa
    13621 tttgtaaaat tcctcctggc ttgctccgcc gtcaatgtat ccggcgtagc catttttaga
    13681 ctgatcgaag aaaatttcct tgtacttctc aggcagttgc tgtctgacaa gggccttcag
    13741 caaagtcaag tcttggtggt gctcatcata gcgcttgatc atactagcgc tcagcggagc
    13801 tttggtgatc tccgtgttca ctcgcagaat atcactcagc agaatggcgt ctgacaggtt
    13861 ctttgccgcc aaaaaaaggt ctgcgtactg gtcgccgatc tgggccagca gattgtcgag
    13921 atcatcatcg taggtgtctt tgctcagttg aagcttggca tcttcggcca ggtcgaagtt
    13981 agatttaaag ttgggggtca gcccgagtga cagggcgata agattaccaa acaggccgtt
    14041 cttcttctcc ccagggagct gtgcgatgag gttttcgagc cgccgggatt tggacagcct
    14101 agcgctcagg attgctttgg cgtcaactcc ggatgcgttg atcgggttct cttcgaaaag
    14161 ctgattgtaa gtctgaacca gttggataaa gagtttgtcg acatcgctgt tgtctgggtt
    14221 caggtccccc tcgatgagga agtgtccccg aaatttgatc atatgcgcca gcgcgagata
    14281 gatcaaccgc aagtcagcct tatcagtact gtctacaagc ttcttcctca gatgatatat
    14341 ggttgggtac ttttcatggt acgccacctc gtccacgata ttgccaaaga ttgggtggcg
    14401 ctcgtgcttt ttatcctcct ccaccaaaaa ggactcctcc agcctatgga agaaagagtc
    14461 atccacctta gccatctcat tactaaagat ctcctgcagg tagcagatcc gattctttct
    14521 gcgggtatat ctgcgccgtg ctgttctttt gagccgcgtg gcttcggccg tctccccgga
    14581 gtcgaacagg agggcgccaa tgaggttctt ctttatgctg tggcgatcgg tattgcccag
    14641 aactttgaat tttttgctcg gcaccttgta ctcgtccgta atgacggccc agccgacgct
    14701 gtttgtgccg atatcgagcc caatggagta cttcttgtcc atttttacaa atccggtcaa
    14761 tttggtggtt tggtgaagct gagtaactga tttaggttta agaacccaga ttaagaaatg
    14821 gagatggcgg gaaaatcgac acgattttgg cgctatccat ttgccaaatc cgaatccaac
    14881 aaacaaacgg gttctcttaa tgggccttac tcctctttaa taaagcccaa aaacaaaaac
    14941 aaaaaagaag caattatgag acatctattt gaacgagatc aagcatcaga gagttgtagg
    15001 agctacctcg gtctgtgaag taattgctga tggagtccac caagtgtaga atctagctaa
    15061 atacagtaaa agaatcaaag tttcagcctg agttctaagt tactgatatt taccgattcg
    15121 aaagtctaaa ctattgctga aactactaat aagttcatat ccaaaatcat tgttctctat
    15181 gataaagccc aattgaaaga gaaaagaaaa aaaaacaatg atgagagatg atgtgttgcc
    15241 gaccacctaa aaccacttta acaaaagcaa aagccaagta aaggttacat tctctgcaac
    15301 ttttttgtca taataaaaac taaacatcta ttaactccaa gcttgaattc ttgcagagac
    15361 cgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
    15421 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
    15481 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
    15541 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
    15601 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
    15661 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
    15721 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
    15781 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
    15841 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
    15901 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
    15961 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
    16021 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga
    16081 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atcgcgtgga
    16141 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
    16201 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
    16261 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
    16321 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
    16381 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
    16441 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
    16501 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
    16561 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
    16621 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
    16681 gtgtgccggt ttccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
    16741 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
    16801 gccagtcggt ctcagcttct gcaggcatgc gacgtcgagc ttagcttgag cttggatcag
    16861 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
    16921 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
    16981 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
    17041 actttaaagt actttaaagt actttaaagt actttgatcc aacccctccg ctgctatagt
    17101 gcagtcggct tctgacgttc agtgcagccg tgttctgaaa acgacatgtc gcacaagtcc
    17161 taagttacgc gacaggctgc cgccctgccc tt
//
