LOCUS       sequence-148622-       18050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18050)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 18050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE167
FEATURES             Location/Qualifiers
     source          1..18050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2125..3192
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3258..3452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3688..3710)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3796..3936
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3851..3870
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3951..3968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4122..4710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4202..4221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4954..5745)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6266..6290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6436..6615
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6651..6706
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6708..7733
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6708..7733
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      7746..7998
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8467..9162)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(10369..11076)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(11087..11107)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11120..15223)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(11120..15223)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(11120..15223)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        16227..16329
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             16330..16989
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(16396..16415)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             17331..17636
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     17515..17534
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    17757..17781
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg
     2161 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     2221 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     2281 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     2341 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     2401 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     2461 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     2521 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     2581 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     2641 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     2701 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     2761 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     2821 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     2881 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     2941 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     3001 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     3061 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     3121 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     3181 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     3241 cgaaaaggtg tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     3301 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     3361 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     3421 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     3481 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     3541 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     3601 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     3661 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     3721 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     3781 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     3841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3901 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     3961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4561 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc
     4801 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     4861 tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac gccggcgaag
     4921 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc
     4981 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     5041 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     5101 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     5161 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     5221 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     5281 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     5341 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     5401 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     5461 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     5521 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     5581 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     5641 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     5701 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt
     5761 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     5821 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     5881 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     5941 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
     6001 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
     6061 aatcagcatg ctaccctccg cgagatcatc cgtgtttaaa acccggcagc ttagttgccg
     6121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     6181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     6241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     6301 ttagacaact taataacaca ttgcggatcc ctcggtcaca tgtgcatcct ctctgggaga
     6361 gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta
     6421 cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt
     6481 ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
     6541 cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc
     6601 ctcggtatcc aattagagtc tcatattcac tctcatactg tatttttaca acaattacca
     6661 acaacaacaa acaacaaaca acattacaat tactatttac aattacaatg aaaaagcctg
     6721 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     6781 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     6841 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     6901 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     6961 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     7021 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     7081 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     7141 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     7201 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     7261 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     7321 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     7381 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     7441 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     7501 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     7561 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     7621 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     7681 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taggcttgtc
     7741 aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     7801 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     7861 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     7921 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     7981 catctatgtt actagatcga cgctccatgg gaattcgtaa agcgactaaa tggagcaacc
     8041 tactgttttt gaagtttggg ttgccatgta atcacttgca ataaatatat gtactaatga
     8101 ctgcatcggc ttgcgtgtct aaatttgtag cttattgaaa actttttgtg gttaaagacg
     8161 aatccagtta actaacataa gtttaacaaa acaagacctt ttccatatat agaccaccgt
     8221 taccaactta caaagaagcc aaataaagta caacacaaca cttgcaagtt acaaaaagag
     8281 aggaaaaaaa acatggtacc atttgacaac atatagaaac acacataaac attacttata
     8341 acaaaaagat tcaaactcgg cttttattat ctccccctga tcaaattcta aacactttat
     8401 tcctcataaa ctaactcccc gacattggag ttattggact atgacgatga catcagtggg
     8461 gtaagctcac ttgtgcccca gtttgctagg gaggtcgcag tatctggcca cagccacctc
     8521 gtgctgctcg acgtaggttt ctttgtcggc ctccttgatt ctttccagtc tgtggtccac
     8581 atagtagacg ccgggcatct tgaggttctt agcgggtttc ttggatctgt atgtggtctt
     8641 gaagttgcag atcaggtggc ccccgcccac gagcttcagg gccatgtcgc ttctgccttc
     8701 caggccgccg tcagcggggt acagcatctc ggtgttggcc tcccagccga gtgttttctt
     8761 ctgcatcaca gggccgttgg atgggaagtt cacccctctg atcttgacgt tgtagatgag
     8821 gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc acgcccccat cttcgtatgt
     8881 ggtgactctc tcccatgtga agccctcagg gaaggactgc ttaaagaagt cggggatgcc
     8941 ctgggtgtgg ttgatgaagg ttctgctgcc gtacatgaag ctggtagcca ggatgtcgaa
     9001 ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg tgccctcgta
     9061 gggcttgcct tcgccctcgg atgtgcactt gaagtggtgg ttgttcacgg tgccctccat
     9121 gtacagcttc atgtgcatgt tctccttaat cagctcgctc atagtagtgt gctggccacc
     9181 acgagtacgg tcacggtcat gttccccacc agtatgttgc tgtccgtagt actgagctct
     9241 gtctttcaga tcctgagctt tgcttcccaa cttcatcctt gcactgtcca acttgtctga
     9301 tccctgtggg tgctctcccg ttgcgtacct attcacaatc aataggtatc ataccaagca
     9361 ttttacctat atagatgata tacacagaca cattcaagct agctaggtca aatttcgtat
     9421 ttagttaacc aaatgttccc tagaatttgc aatttcttat tgttacattt gttattcgac
     9481 caaaaaaatt gatccaaagc tactggctca acacatgcat gcacatattg cacaaaatta
     9541 tgaagtaaga tgataaatgt gtgcttactt gtaaatccaa gagaaaacgg ttatagcggc
     9601 aatgccaaac cctccagagg aaagaaaacc ggtgatgagg agtgcaactg tgatgagagc
     9661 cgggacaagg attgggctga agataacgag cagaggtgtt gcaacagtca aagctatgac
     9721 agttccaaca agggtaaggc tggagagaac aaggagggaa ccaccagctg tgacagcagt
     9781 tgcagcttta gcaatctgcc tagacttgga gtagtcagat cctcgtccgg acatctggta
     9841 ctggtcacga tctcggccca tcatcgggta ctgatctctg ccgatgatat cgtgatgggt
     9901 tcctctagct gtatccgcca tttttttgtt cttgtttact agagagaatg agattttgaa
     9961 tgagagattg atcaaaaaga tgtgtatata ttttggaaga ggtaggagtg tttgttagat
    10021 atattaagaa aggagacgag aagtcacgtg tcaatggaga catcatgcaa ttgtaggcca
    10081 cgtgttaatg gcgtcaccat gcagccgctg catgtgttaa cgagaggagt ctattcaaag
    10141 acacgtttgt tttttgccta gctcagaatg tggcgttggc atcgaaccga aacataaaac
    10201 gcaaaacgaa cctttgggct cgtcagtatg ggtttgggcc ttcaatagat tccttctata
    10261 cggcccacat atccactatt ggcaacaaaa ttagaaaaat ttaggattta gttttaaaat
    10321 ttgttccgcg acattctaga actccgagct cgaattctag tagcggtcct gctgagcctc
    10381 gacatgttgt cgcaaaattc gccctggacc cgcccaacga tttgtcgtca ctgtcaaggt
    10441 ttgacctgca cttcatttgg ggcccacata caccaaaaaa atgctgcata attctcgggg
    10501 cagcaagtcg gttacccggc cgccgtgctg gaccgggttg aatggtgccc gtaactttcg
    10561 gtagagcgga cggccaatac tcaacttcaa ggaatctcac ccatgcgcgc cggcggggaa
    10621 ccggagttcc cttcagtgag cgttattagt tcgccgctcg gtgtgtcgta gatactagcc
    10681 cctggggcac ttttgaaatt tgaataagat ttatgtaatc agtcttttag gtttgaccgg
    10741 ttctgccgct ttttttaaaa ttggatttgt aataataaaa cgcaattgtt tgttattgtg
    10801 gcgctctatc atagatgtcg ctataaacct attcagcaca atatattgtt ttcattttaa
    10861 tattgtacat ataagtagta gggtacaatc agtaaattga acggagaata ttattcataa
    10921 aaatacgata gtaacgggtg atatattcat tagaatgaac cgaaaccggc ggtaaggatc
    10981 tgagctacac atgctcaggt tttttacaac gtgcacaaca gaattgaaag caaatatcat
    11041 gcgatcatag gcttctcgca tatctcatta aagcaggaca agctcacacc ttcctcttct
    11101 tcttggggtc agccctgctg tctccaccga gctgagagag gtcgattctt gtttcataga
    11161 gccccgtaat tgactgatga atcagtgtgg cgtccaggac ctcctttgta gaggtgtacc
    11221 gctttctgtc tatggtggtg tcgaagtact tgaaggctgc aggcgcgccc aagttggtca
    11281 gagtaaacaa gtggataatg ttttctgcct gctccctgat gggcttatcc ctgtgcttat
    11341 tgtaagcaga aagcacctta tcgaggttag cgtcggcgag gatcactctt ttggagaatt
    11401 cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg ttgttccacg aacagctgct
    11461 tctgctcatt atcttcggga gatcctttga gcttttcata gtggctggcc agatacaaga
    11521 aattaacgta tttagagggc agtgccagct cgttaccttt ctgcagctcg cccgcactag
    11581 cgagcattcg tttccggccg ttttcaagct caaagagaga gtacttggga agcttaatga
    11641 tgaggtcttt tttgacctct ttatatcctt tcgcctcgag aaagtcgatg gggttttttt
    11701 cgaagcttga tcgctccatg attgtgatgc ccagcagttc cttgacgctt ttgagttttt
    11761 tagacttccc tttctccact ttggccacaa ccagtacact gtaagcgact gtaggagaat
    11821 cgaatccgcc gtatttcttg gggtcccaat cttttttgcg tgcgatcagc ttgtcgctgt
    11881 tccttttcgg gaggatactt tccttggaga agcctccggt ctgtacttcg gtctttttaa
    11941 cgatgttcac ctgcggcatg gacaggacct tccggactgt cgcgaaatcc ctacccttgt
    12001 cccacacgat ttctcctgtt tctccgtttg tttcgataag tggtcgcttc cgaatctctc
    12061 cattggccag tgtaatctcg gtcttgaaaa aattcataat attgctgtaa aagaagtact
    12121 tagcggtggc cttgcctatt tcctgctcag actttgcgat cattttccta acatcgtaca
    12181 ctttatagtc tccgtaaaca aattcagatt caagcttggg atattttttg ataagtgcag
    12241 tgcctaccac tgcattcagg taggcatcat gcgcatggtg gtaattgttg atctctctca
    12301 ccttataaaa ctgaaagtcc tttctgaaat ctgaaaccag cttagacttc agagtaataa
    12361 ctttcacctc tcgaatcagt ttgtcatttt catcgtactt ggtgttcatg cgtgaatcga
    12421 gaatttgggc cacgtgcttg gtgatctggc gtgtctcaac aagctgcctt ttgatgaagc
    12481 cggctttatc caactcagac aggccacctc gttcagcctt agtcagatta tcgaacttcc
    12541 gttgtgtgat cagtttggcg ttcagcagct gccgccaata atttttcatt ttcttgacaa
    12601 cttcttctga ggggacgtta tcactcttcc ctctattttt atcggatctt gtcaacactt
    12661 tattatcaat agaatcatct ttgagaaaag actggggcac gatatgatcc acgtcgtagt
    12721 cggagagccg attgatgtcc agttcctgat ccacgtacat gtccctgccg ttctgcaggt
    12781 agtacaggta gagcttctca ttctgaagct gggtgttttc aactgggtgt tccttaagga
    12841 tttgggaccc cagttctttt ataccctctt caatcctctt catcctttcc ctactgttct
    12901 tctgtccctt ctgggtagtt tggttctctc gggccatctc gataacgata ttctcgggct
    12961 tatgccttcc cattactttg acgagttcat ccacgacctt aacggtctgc agtattccct
    13021 ttttgatagc tgggctacct gcaagattag cgatgtgctc gtggagactg tccccctggc
    13081 cagaaacttg tgctttctgg atgtcctcct taaaggtgag agagtcatca tggatcaact
    13141 gcatgaagtt ccggttggca aatccatcgg acttaagaaa atccaggatt gtctttccac
    13201 tctgcttgtc tcggatccca ttgatcagtt ttcttgacag ccgcccccat cctgtatatc
    13261 ggcgcctctt gagctgtttc atgactttgt cgtcgaagag atgagcgtaa gttttcaagc
    13321 gttcttcaat catctcccta tcttcaaaca acgtaagggt gaggacaatg tcctcaagaa
    13381 tgtcctcgtt ctcctcattg tccaggaagt ccttgtcttt aatgattttc aggagatcgt
    13441 gatacgttcc cagggatgcg ttgaagcgat cctccactcc gctgatttca acagagtcga
    13501 aacattcaat ctttttgaaa taatcttctt tgagctgttt cacggtaact ttccggttcg
    13561 tcttgaagag gaggtccacg atagctttct tctgctctcc agacaggaat gctggctttc
    13621 tcatcccttc tgtgacgtat ttgaccttgg tgagctcgtt ataaactgtg aagtactcgt
    13681 acagcagaga gtgtttagga agcacctttt cgttaggcag atttttatca aagttagtca
    13741 tcctttcgat gaaggactgg gcagaggccc ccttatccac gacttcctcg aagttccagg
    13801 gagtgatagt ctcttctgat ttgcgagtca tccacgcgaa tctggaattt ccccgggcga
    13861 gggggcctac atagtagggt atccgaaatg tgaggatttt ctcaatcttt tccctgttat
    13921 ctttcaaaaa ggggtagaaa tcctcttgcc gcctgaggat agcgtgcagt tcgcccaggt
    13981 gaatctggtg ggggatgctt ccattgtcga aagtgcgctg tttgcgcaac agatcttctc
    14041 tgttaagctt taccagcagc tcctcggtgc cgtccatttt ttccaagatg ggcttaataa
    14101 atttgtaaaa ttcctcctgg cttgctccgc cgtcaatgta tccggcgtag ccatttttag
    14161 actgatcgaa gaaaatttcc ttgtacttct caggcagttg ctgtctgaca agggccttca
    14221 gcaaagtcaa gtcttggtgg tgctcatcat agcgcttgat catactagcg ctcagcggag
    14281 ctttggtgat ctccgtgttc actcgcagaa tatcactcag cagaatggcg tctgacaggt
    14341 tctttgccgc caaaaaaagg tctgcgtact ggtcgccgat ctgggccagc agattgtcga
    14401 gatcatcatc gtaggtgtct ttgctcagtt gaagcttggc atcttcggcc aggtcgaagt
    14461 tagatttaaa gttgggggtc agcccgagtg acagggcgat aagattacca aacaggccgt
    14521 tcttcttctc cccagggagc tgtgcgatga ggttttcgag ccgccgggat ttggacagcc
    14581 tagcgctcag gattgctttg gcgtcaactc cggatgcgtt gatcgggttc tcttcgaaaa
    14641 gctgattgta agtctgaacc agttggataa agagtttgtc gacatcgctg ttgtctgggt
    14701 tcaggtcccc ctcgatgagg aagtgtcccc gaaatttgat catatgcgcc agcgcgagat
    14761 agatcaaccg caagtcagcc ttatcagtac tgtctacaag cttcttcctc agatgatata
    14821 tggttgggta cttttcatgg tacgccacct cgtccacgat attgccaaag attgggtggc
    14881 gctcgtgctt tttatcctcc tccaccaaaa aggactcctc cagcctatgg aagaaagagt
    14941 catccacctt agccatctca ttactaaaga tctcctgcag gtagcagatc cgattctttc
    15001 tgcgggtata tctgcgccgt gctgttcttt tgagccgcgt ggcttcggcc gtctccccgg
    15061 agtcgaacag gagggcgcca atgaggttct tctttatgct gtggcgatcg gtattgccca
    15121 gaactttgaa ttttttgctc ggcaccttgt actcgtccgt aatgacggcc cagccgacgc
    15181 tgtttgtgcc gatatcgagc ccaatggagt acttcttgtc cattgctgca catacataac
    15241 atatcaagat cagaacacac atatacacac acaaatacaa tcaagtcaac aactccaaaa
    15301 agtccagatc tacatatata catacgtaaa taacaaaatc atgtaaataa tcacaatcat
    15361 gtaatccaga tctatgcaca tatatatata cacaattaat aaaaaaaatg atataacaga
    15421 tctatatcta tgtatgtaac aacacaatca gatgagagaa gtgatgtttt cagatctgta
    15481 tacatacaaa cacaaacaga tgaacaattg atacgtagat ccatatgtat acgtacaatt
    15541 agctacacga ttaaatgaaa aaaatcaacg atttcggatt ggtacacaca aacgcaacaa
    15601 tatgaagaaa ttcatatctg attagatata aacataacca cgtgtagata cacagtcaaa
    15661 tcaacaaatt tatagcttct aaacggatga gatgaacaag ataaagatat tcacataagg
    15721 catacataag ataagcagat taacaaacta gcaataatac atacctaatt aaaacaagga
    15781 ataacagaga gagagagaga gagagagatt taccttgaaa atgaagagga gaagagagga
    15841 tttcttaaaa ttgggggtag agaaagaaag atgatgaatt gtgagaaagg agagatagaa
    15901 gggggggttg tatatatagg ctgtagaaga ttatttttgt gtttgaggcg gtgaaggaag
    15961 aggggatctg actatgacac gtttgcggtt acgtatttcg ataggagtct ttcaacgctt
    16021 aacgccgtta ctctatatga ccgtttgggc cgtaacgggg ccgtttgtta acgctgatgt
    16081 tgattctttt ctttctttct ttcttccttt tttaaagaag caattgtaca atcgttgcta
    16141 gctgtcaaac ggataattcg gatacggata tgcctatatt catatccgta atctccaagc
    16201 ttgaattctt gcagagaccg agacgttgat cggcacgtaa gaggttccaa ctttcaccat
    16261 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
    16321 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
    16381 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
    16441 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
    16501 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
    16561 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
    16621 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
    16681 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
    16741 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
    16801 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
    16861 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat
    16921 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
    16981 ggggcgtaat cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
    17041 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
    17101 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
    17161 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
    17221 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
    17281 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
    17341 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
    17401 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
    17461 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
    17521 tgaccaccga tatggccagt gtgccggttt ccgttatcgg ggaagaagtg gctgatctca
    17581 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
    17641 caggctccct tatacacagc cagtcggtct cagcttctgc aggcatgcga cgtcgagctt
    17701 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
    17761 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
    17821 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
    17881 tcccctcggg atcaaagtac tttaaagtac tttaaagtac tttaaagtac tttgatccaa
    17941 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtg ttctgaaaac
    18001 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt
//
