LOCUS       sequence-148653-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shCDK4 targeting 3UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Hong AL, Tseng YY, Cowley GS, Jonas O, Cheah JH, Kynnap BD, Doshi 
            MB, Oh C, Meyer SC, Church AJ, Gill S, Bielski CM, Keskula P, 
            Imamovic A, Howell S, Kryukov GV, Clemons PA, Tsherniak A, Vazquez 
            F, Crompton BD, Shamji AF, Rodriguez-Galindo C, Janeway KA, Roberts 
            CW, Stegmaier K, van Hummelen P, Cima MJ, Langer RS, Garraway LA, 
            Schreiber SL, Root DE, Hahn WC, Boehm JS
  TITLE     Integrated genetic and pharmacologic interrogation of rare cancers.
  JOURNAL   Nat Commun. 2016 Jun 22;7:11987. doi: 10.1038/ncomms11987.
  PUBMED    27329820
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-22"; volume: "7:11987. doi"; pages: " 
            10.1038/ncomms11987"
COMMENT     Sequence Label: TRCN0000297275
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    299..416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        465..948
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     895..913
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaggta ccggccttcc catttctcta cactactcga gtagtgtaga
      241 gaaatgggaa ggtttttgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
      301 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
      361 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
      421 tttattacag ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggttgcgc
      481 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
      541 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
      601 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
      661 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
      721 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
      781 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
      841 gtgcgggagg cgggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
      901 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgt
//
