LOCUS       sequence-148654-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shCDK4 targeting 3UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Hong AL, Tseng YY, Cowley GS, Jonas O, Cheah JH, Kynnap BD, Doshi 
            MB, Oh C, Meyer SC, Church AJ, Gill S, Bielski CM, Keskula P, 
            Imamovic A, Howell S, Kryukov GV, Clemons PA, Tsherniak A, Vazquez 
            F, Crompton BD, Shamji AF, Rodriguez-Galindo C, Janeway KA, Roberts 
            CW, Stegmaier K, van Hummelen P, Cima MJ, Langer RS, Garraway LA, 
            Schreiber SL, Root DE, Hahn WC, Boehm JS
  TITLE     Integrated genetic and pharmacologic interrogation of rare cancers.
  JOURNAL   Nat Commun. 2016 Jun 22;7:11987. doi: 10.1038/ncomms11987.
  PUBMED    27329820
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-22"; volume: "7:11987. doi"; pages: " 
            10.1038/ncomms11987"
COMMENT     Sequence Label: TRCN0000297275
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..630)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(114..134)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     611..630
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(715..733)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 atccagaggt tgattgtcga cttaacgcgt tcaggcaccg ggcttgcggg tcatgcacca
       61 ggtgcgcggt ccttcgggca cctcgacgtc cgcggtgacg gtgaagccga gccgctcgta
      121 gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc
      181 cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga
      241 gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc
      301 ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat
      361 ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccacggcggc
      421 gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc
      481 ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg
      541 gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc
      601 gaggcgcacc gtgggcttgt actcggtcat ggtaagctcc ggtggatccc cctggggaga
      661 gaggtcggtg attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca
      721 gaatgcggaa caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc
      781 ccgcaccgcc ccttcccggc cgctgctctc ggcgcgccct gctgagcagc cgctattggc
      841 cacagcc
//
