LOCUS       sequence-148656-       14790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP for monosynaptic circuit mapping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14790)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 14790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-EGFP
FEATURES             Location/Qualifiers
     source          1..14790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3325..3327,3328..3330,3331..4044)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3325..3327,3328..3330,3331..4041)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3370..3391)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3631..3650)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3978..3999
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6219..6230
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8022..8033)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11230..11248)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11244..11291
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11319..11335)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11319..11335)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11332..11354)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11343..11359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11367..11373,11374..11391,11392..11397))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11412..11433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11550..11567)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11721..12309)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11801..11820)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12480..13271,13272..13340))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13103..13122
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13341..13445)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13472..13927
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13559..13578)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13769..13790
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14053..14075
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14067..14084
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14068..14084
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14154..14457
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14458..14661
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     14612..14632
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     14706..14725
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14706..14724
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggagctgtt caccggggtg
     3361 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3481 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3541 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3601 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3661 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3721 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3781 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3841 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3901 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3961 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4021 ggcatggacg agctgtacaa gtaagctagc ggcgcctagc cggtcatcct tttgacgatt
     4081 ccagtcccga ggataacctc ctctcgggat tggggggaat ctttggatcc agtagtcctc
     4141 cttgaactcc gtccaacagg gcagattcaa gagtcataag actttcatta atcatttcag
     4201 ttgatcagac atggtcgtgt agattctctt aatacgggag atcttctagc agtttcagtg
     4261 accaacggtg ctttcattct ccaggaactg ataccaaagg ttgtggacag gccaaggggt
     4321 acttcggatg actctgtgct tgggcacaga aagaggtcgt agtgtgcccc ccgatagcgg
     4381 actcgacatg aatcaactaa gaaaggcaat ctgcctccca tgatggacat aagcaatagt
     4441 tcacaaccat cttgcatctc agtgaagtgt acataactat tgagggctgg gtcatctaag
     4501 catttcagtc gagaaaaaaa ctgtagacca aaagaacaac tagcaacact tctcatccag
     4561 agacccatat caagatgcta gatccgggag aggtttatga tgaccctatt gatccaattg
     4621 agtcagaggc tgaacccaga ggaaccccca ctgtccccaa catcttgagg aactccgact
     4681 acaatctcaa ttctcctttg atagaggatc ctgccaaact aatgttagaa tggttgaaga
     4741 cagggaacag accttatcgg atgactttga cagacaattg ctccaggtct tacaaagttt
     4801 tgaaagatta tttcaagaaa gtagatttgg gttctctcaa agtgggcgga actgctgcac
     4861 agtcaatggt ttctctctgg ttgtgtggtg cccactctga atcaaacagg agccggagat
     4921 gtataaccga cttggcccat ttctattcca agtcatcccc catagagaag ctattgaatt
     4981 gtacgctagg aaacagaggc ctgagaatcc caccagaggg ggtgttaaat tgcctcgaga
     5041 gggtcaatta tgacaaggca tttgggaggt atctggccaa cacgtattcc tcttacttgt
     5101 ttttccatgt aatcacctta tacatgaatg ccttagactg ggaagaggaa aagaccatcc
     5161 tggcattatg gaaagatata acctcagtgg ataccgagaa ggacttggtc aaattcaaag
     5221 atcaaatatg gggactgttg attgtgacaa aggactttgt ttactctcag agttcaaact
     5281 gtctttttga cagaaactac acactgatgc taaaggatct tttcttgtct cgattcaact
     5341 ccttaatgat tctgctttct ccccctgagc cccgatactc agatgactta atatctcagc
     5401 tgtgccagct atacatcgct ggggatcaag tcttgtccat gtgtgggaac tccggctatg
     5461 aagtcatcaa aatattggag ccatatgtcg tgaacagttt ggtccagagg gcagagaagt
     5521 ttaggcctct catccacccc ttgggagact ttcctatgtt cataaaagac aaggtgaatc
     5581 aacttgaagg gacttttggt cccagtgcaa agaggttttt tagggtttta gatcaattcg
     5641 acaacataca tgacttagta tttgtgtatg gctgttacag acattggggg cacccctata
     5701 tagattatcg gaagggtctg tcgaaactat atgatcaagt tcacattaag aaagtaatag
     5761 ataagtccta ccaggagtgt ttagcaagtg acttggccag aaggatcctc agatggggat
     5821 ttgacaagta ctccaaatgg tatctagatt cgagattcct tgcccgagac caccccttga
     5881 ctccttatgt caagacccaa acatggccac ccaaacatat agtagacttg gtgggggaca
     5941 catggcataa gctcccgatc acgcagatct ttgaaattcc tgaatcaatg gacccgtcag
     6001 agatactgga tgacaaatca cattctttca ccagaacaag gttagcttct tggctgtcag
     6061 agaaccgagg ggggcctgtt cctagcgaga aggtcattat cacggccctg tctaagccac
     6121 ctgtcaatcc ccgagagttt ttgaaatcta tagacctcgg aggattgcca gatgaggatt
     6181 tgataattgg cctcaaacca aaggaacggg agttgaagat cgagggccga ttctttgctc
     6241 taatgtcatg gaatctaaga ttatattttg tcatcaccga aaagctcttg gccaactaca
     6301 ttttgccact ttttgacgca ctgactatga cagacaacct gaacaaggtg ttcaaaaagt
     6361 tgatcgacag ggtcaccggg caagggcttt tggactattc tagggtcaca tacgcatttc
     6421 acctggacta tgagaaatgg aacaatcatc aaagattgga gtcaacagag gatgtattct
     6481 ctgtcctaga tcaggtgttt ggattgaaga gggtgttttc tagaacacac gagttttttc
     6541 agaagtcctg gatctattat tcagacagat cagacctcat tgggttatgg gaggaccaaa
     6601 tatattgctt ggatatgtct aacggcccaa cctgctggaa tggccaagat ggcgggctag
     6661 agggcttgcg gcagaagggc tggagtctag tcagtttatt aatgatagat agagaatctc
     6721 aaaccaggaa cacaagaacc aagatactag ctcaaggaga caaccaggtt ctgtgtccta
     6781 catacatgtt gtcaccggga ttgtctcaag aggggcttct ctatgagtta gagagcatat
     6841 caaggaatgc actctcaata taccgagcta tcgaggaagg agcatctaag ctggggctga
     6901 tcatcaagaa ggaagagacc atgtgtagtt atgactttct catatatggg aagaccccct
     6961 tatttcgagg caacatattg gtgcctgaat ccaaaagatg ggcccgagtc tcttgcatct
     7021 ctaacgacca aatagtcaac ctcgccaata taatgtcgac agtatccacc aatgcgctga
     7081 cagtggcaca acactctcaa tctctgatca aacctatgag ggattttctg ctcatgtcag
     7141 tacaggcagt tttccactac ctgttgttta gcccaatcct aaaaggcaga gtttataaga
     7201 ttctgagtgc tgaaggggag agctttctcc tagccatgtc gcggataatc tacctagatc
     7261 cttctttggg aggggtgtct ggaatgtctc tcgggaggtt ccatatacgt cagttctcag
     7321 accctgtctc tgaagggttg tcattctgga gagagatctg gttaggctct catgagtcct
     7381 ggattcacgc gttgtgtcag gaggccggga accccgatct tggagagaga acactcgaga
     7441 gcttcactcg ccttttagaa gatcctacta ccttaaatat caaaggaggg gccagcccta
     7501 ccattctact caaggatgct atcagaaagg ctctgtacga cgaggtggac aaggtggaaa
     7561 attcagagtt tcgagaggca atcctgctgt ccaagaccca tagagataac tttatactct
     7621 ttttaaaatc tgttgagcct ctgttccctc gatttctcag tgagctcttc agttcgtctt
     7681 tcttgggaat accggagtca atcattggac tgatacaaaa ctcccggaca ataagaaggc
     7741 agtttagaaa gagtctctca agaactttag aagagtcctt ctacaactca gagatccacg
     7801 ggattaatcg gataacccag acacctcaaa gggtcggaag ggtgtggcct tgctcttcag
     7861 agagggcgga tctacttagg gagatctctt gggggaggaa agtggtaggc acgacagttc
     7921 ctcacccttc cgagatgttg gagttgtttc ccaaatcctc catttcctgc acttgtggag
     7981 caacaggggg aggcaatcct agagtctctg tatcagtact cccgtccttc gatcagtcat
     8041 ttttctcacg gggcccccta aagggatact tgggctcgtc cacctctatg tcaacccagc
     8101 tattccatgc atgggaaaaa gtcactaatg ttcatgtggt gaaaagggct ctatcgttaa
     8161 aagaatctat aaactggttc atcaatagga attccaattt ggctcaaact ctaattggaa
     8221 acatcatgtc tctgacaggc cctgacttcc ctctagaaga ggcccctgtt ttcaaacgga
     8281 cagggtcagc cttgcatagg ttcaagtctg ccagatacag cgaaggaggg tattcttctg
     8341 tttgccctaa ccttctctcc catatctctg ttagtacaga cactatgtct gatttgaccc
     8401 aaaacgggaa gaactatgat ttcatgtttc agccattgat gctttatgcg caaacatgga
     8461 catcggaact ggtacagagg gatacaagac ttagagactc cacgtttcac tggcaccttc
     8521 ggtgcaacag atgtgtgagg cccattgatg atataacact ggaaacttct cagatcttcg
     8581 agttcccgga tgtgtcaaaa aggatatcca ggatggtttc tggagctgtc cctcaatttc
     8641 agaagcttcc tgatatccgt ctaagaccag gtgattttga atccctaagt ggtagagaaa
     8701 agtctcgcca tatagggtca gctcaggggc tcttatactc aatcttagta gcaattcacg
     8761 actcaggata caatgatggg accatcttcc ctgtcaacat atacggcaaa gtttccccca
     8821 gagactattt gagagggctt gcaagaggga tcttgatagg gtcctcgatt tgcttcttga
     8881 cacgaatgac aaatattaac attaacagac ctcttgaatt gatctcaggg gtaatttcct
     8941 atattctcct gaggctggat aatcatccct ctctgtatat aatgctcaga gaaccgtctc
     9001 ttagaggaga aatattctct atccctcaga aaatccccgc cgcttaccca accactatga
     9061 gagaaggcaa cagatcgatt ttgtgttacc tccaacacgt gctacgctat gagcgagagg
     9121 caatcacggc gtccccggag aatgactggc tgtggatctt ctcagacttc agaagtgtga
     9181 aaatgacgta cttgaccctc attacttacc agtctcacct cctactccag agggttgaga
     9241 gaaacttgtc taagagtatg agagctactc tgcgacaaat gggttcctta atgaggcaag
     9301 tgctgggtgg gcacggagaa gataccttgg agtcagacga tgacattcaa cgattactaa
     9361 aagactcttt gcgaaagaca aggtgggtgg atcaagaggt gcgccatgca gctagaacca
     9421 tgaatggaga ttacagcccc gacaagaaag tatcccgtaa ggtaggatgt tcagaatggg
     9481 tctgctctgc tcaacagatt gccgtctcca cctcagccaa cccggcccct gtctcagagc
     9541 ttgacattag ggccctctct aagcggtttc aaaacccctt gatctcgggc ctgagagtgg
     9601 ttcagtgggc aaccggtgcc cattataagc ttaagcctat tctagatgat ctaaatgttt
     9661 tcccatctct ctgtcttgta attggagacg ggtcaggggg aatatcaagg gcagttctca
     9721 acatgtttcc agattctaag cttgtgttca acagcctatt ggaggtgaat gatctgatgg
     9781 cttccggaac acatccactg cctccttcag caatcatgag tggaggagat gacatcatct
     9841 ccagagtgat agactttgac tcaatatggg agaaaccgtc cgacctgagg aactcggcca
     9901 cctggagata cttccagtcg gttcaaaaac aggtcaacat gtcgtatgac ctcattattt
     9961 gtgatgcaga agttactgat attgcatcta tcaaccggat aactctgttg atgtctgatt
    10021 tcgcattgtc tatagatgga ccactttatc tggtcttcaa aacttacggg actatgctag
    10081 taaacccgga ctataaagct attcaacatc tgtcaagagc gttcccttcg gtcacagggt
    10141 ttgtaaccca agtaacttca tccttttctt ctgagctata cctccggttc tctaaacgag
    10201 gaaagttttt cagggacgcc gagtacttga cctcttccac ccttcgagag atgagccttg
    10261 tgttgttcaa ttgcagcagc cccaaaagtg agatgcagag agctcgttcc ttaaactatc
    10321 aagacctggt aaggggattt cctgaagaga tcatatcaaa tccttacaac gaaatgatca
    10381 taactctgat tgacaatgat gtagagtcct tcctagtcca caagatggtg gatgatcttg
    10441 agctacagag gggaactctg tctaaagtgg ctatcattat atccatcatg atcgtttttt
    10501 ccaatagagt cttcaacatt tccaaacctt tgactgaccc cttgttctat ccaccgtctg
    10561 atcctaaaat cctgaggcac ttcaacatat gttgcagtac tatgatgtat ctatctaccg
    10621 ctttaggcga cgtccctaac ttcgcaagac ttcatgacct gtataacaga cctataactt
    10681 gttacttcag aaaacaagtt attcgaggga atatttatct atcttggagt tggtccgatg
    10741 ataccccagt gttcaagaga gtagcctgta attctagctt gagtctgtca tctcactgga
    10801 tcaggttgat ctacaagata gtgaagacta ccagactcat tggcagcata aaagacctat
    10861 caggagaggt agaacgacat cttcatgggt ataacagatg gatcaccctc gaggatatcc
    10921 gatctagatc atccctacta gattacagtt gtttgtaagc cggatattac cgaaagcctg
    10981 tgcatgctaa aattcttgta tgatgcatct tgaaaaaaac aagatcttga atccggacct
    11041 ctggttgttt gattgttttt tccatcttta ttgttttttt gttaagcgtg ggtcggcatg
    11101 gcatctccac ctcctcgcgg tccgacctgg gcatccgaag gaggacgcac gtccactcgg
    11161 atggctaagg gaggggcggg gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
    11221 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
    11281 ggggtttttt gctgaaagtc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
    11341 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    11401 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    11461 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    11521 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    11581 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    11641 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    11701 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11761 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11821 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11881 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11941 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    12001 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    12061 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    12121 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    12181 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    12241 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    12301 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    12361 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    12421 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    12481 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    12541 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    12601 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    12661 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    12721 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    12781 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    12841 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12901 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12961 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    13021 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    13081 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    13141 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    13201 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    13261 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    13321 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    13381 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    13441 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
    13501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    13561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    13621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    13681 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    13741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    13801 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    13861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    13921 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    13981 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    14041 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcctagttat
    14101 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    14161 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    14221 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    14281 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    14341 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    14401 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
    14461 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
    14521 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
    14581 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
    14641 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
    14701 gaaattaata cgactcacta tagggagacc caagctggct agattaagcg tctgatgagt
    14761 ccgtgaggac gaaacccggc gtaccgggtc
//
