LOCUS       sequence-148661-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating LexA::GADfluw lines from MiMIC lines with inserts into 
            coding introns in Phase 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..25
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7..25
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    65..134
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 gcgcgcaatt aaccctcact aaagggaaca aaagctgggt accatgcatg atgtaggtca
       61 cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact
      121 ccacctcacc catctggtcc atcatgatgg tcgacaagct tagaagttca aatgggcttt
      181 catatttact tttttctctg agtgggtaag gtcattgtgg ggtcttaagg gctaaagggt
      241 agtgatgatg aaggcatttg atgccattgt tcaataactt acctcgccgg aacctccgct
      301 tccaccgcta cctccggatc tttaattaaa aacgagtttt taagcaaact cactccctga
      361 caataaaaac gaattatctt taaataaata tattctattt attaaccaag tagctaatat
      421 agctaaaatc aatttgttgc taacttaaaa ctaacataat tatgtattag ttatttacat
      481 tcttttggtt ggttacaacc aaataaataa tatcactttt aaaaacttaa gccagaactg
      541 aataatttct atttggcttt agtcgacggt atcgataagc ttctgaataa gccctcgtaa
      601 tatattttca tgaagaattt aggtccaaaa aaaagatggg cattaattct agtcatttaa
      661 aaaattctat agatcagagg ttacatggcc aagattgaaa cttagaggag tatagttaca
      721 taaaagaagg caaaacgatg tataaatgaa agaaattgag atggtgcacg atgcacagtt
      781 gaagtgaact tgcgg
//
