LOCUS       sequence-148668-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCS4+-NLS-EGFP-T2A-mCherry-CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, 
            Choi SY
  TITLE     High Cleavage Efficiency of a 2A Peptide Derived from Porcine 
            Teschovirus-1 in Human Cell Lines, Zebrafish and Mice.
  JOURNAL   PLoS One. 2011  . 6(4):e18556.
  PUBMED    21602908
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(4):e18556."
COMMENT     Sequence Label: pSYC-102
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..93
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             103..810
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(248..266)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     516..535
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     710..733
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gagctgtaca agcttaagga gctcgcatgc ggaagcggag agggcagagg aagtctgcta
       61 acatgcggtg acgtcgagga gaatcctgga cctagatcta ccatggtgag caagggcgag
      121 gaggacaaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
      181 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      241 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
      301 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      361 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      421 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      481 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      541 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
      601 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac
      661 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gctggacatc
      721 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac
      781 tccaccggcg gcatggacga gctgtacaag aagctgaacc ctcctgatga gagtggcccc
      841 ggctgcatga gctgcaagtg tgtgctctcc tgagcta
//
