LOCUS       sequence-148671-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  alias pFA6a-mCherry-kanMX6 - vector for C-terminal tagging of yeast 
            genes with mCherry and G418 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pFM571
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..749
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(184..202)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     452..471
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgcaggtcga cggtgcaggt gctggagctg gtgctggtat ggtgagcaag ggcgaggagg
       61 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      121 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      181 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      241 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      301 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      361 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      421 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      481 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      541 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      601 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
      661 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      721 ccggcggcat ggacgagctg tacaagtaag gatccgctag ggataacagg gtaatataga
      781 tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
      841 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
      901 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg
      961 gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagg
//
