LOCUS       sequence-148672-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  alias pFA6a-mCherry-kanMX6 - vector for C-terminal tagging of yeast 
            genes with mCherry and G418 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pFM571
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(61..80)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      809..1006
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 aggaaaagac tcacgtttcg aggccgcgat taaattccaa catggatgct gatttatatg
       61 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
      121 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      181 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      241 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggcaaaa
      301 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      361 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      421 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      481 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc
      541 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
      601 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
      661 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
      721 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
      781 tgatgctcga tgagtttttc taatcagtac tgacaataaa aagattcttg ttttcaagaa
      841 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
      901 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
      961 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
     1021 ctaacgccgc catcc
//
