LOCUS       sequence-148673-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast 
            genes with mCherry and hygromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pFM689
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..759
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(194..212)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     462..481
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggtgcaggt gctggagctg gtgctggtat ggtgagcaag
       61 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      121 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      181 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      241 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      301 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      361 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
      421 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      481 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      541 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
      601 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
      661 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
      721 cgccactcca ccggcggcat ggacgagctg tacaagtaag gatccccggg ttaattaagg
      781 cgcgccagat ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag
      841 gcccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt
      901 cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat acattttgat
      961 ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc ggacctgcga gcagggaaac
     1021 gctcccctca
//
