LOCUS       sequence-148680-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  alias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast 
            genes with mCherry and nourseothricin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pFM699
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..751
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(186..204)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     454..473
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctgcaggtc gacggtgcag gtgctggagc tggtgctggt atggtgagca agggcgagga
       61 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
      121 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      181 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
      241 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      301 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      361 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      421 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
      481 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
      541 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
      601 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
      661 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
      721 caccggcggc atggacgagc tgtacaagta aggatccccg ggttaattaa ggcgcgccag
      781 atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa
      841 taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta
      901 catttagccc atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac
      961 ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct
     1021 c
//
