LOCUS       sequence-148681-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  alias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast 
            genes with mCherry and nourseothricin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pFM699
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..564
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="TLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTLR
                     EVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRLT
                     VEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLCG
                     LDTALYDGTASDGEQALYMSMPCP"
     terminator      572..759
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(627..651)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(951..970)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(952..970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actcttgacg acacggctta ccggtaccgc accagtgtcc cgggggacgc cgaggccatc
       61 gaggcactgg atgggtcctt caccaccgac accgtcttcc gcgtcaccgc caccggggac
      121 ggcttcaccc tgcgggaggt gccggtggac ccgcccctga ccaaggtgtt ccccgacgac
      181 gaatcggacg acgaatcgga cgacggggag gacggcgacc cggactcccg gacgttcgtc
      241 gcgtacgggg acgacggcga cctggcgggc ttcgtggtcg tctcgtactc cggctggaac
      301 cgccggctga ccgtcgagga catcgaggtc gccccggagc accgggggca cggggtcggg
      361 cgcgcgttga tggggctcgc gacggagttc gcccgcgagc ggggcgccgg gcacctctgg
      421 ctggaggtca ccaacgtcaa cgcaccggcg atccacgcgt accggcggat ggggttcacc
      481 ctctgcggcc tggacaccgc cctgtacgac ggcaccgcct cggacggcga gcaggcgctc
      541 tacatgagca tgccctgccc ctaatctcga ggcgaatttc ttatgattta tgatttttat
      601 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
      661 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
      721 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgccgagca aatgcctgca
      781 aatcgctccc catttcaccc aattgtagat atgctaactc cagcaatgag ttgatgaatc
      841 tcggtgtgta ttttatgtcc tcagaggaca acacctgttg taatcgagct cgaattcatc
      901 gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg
      961 agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcgg
//
