LOCUS       sequence-148682-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRSETa (Invitrogen) plasmid expressing Amrose variant 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Schoetz U, Deliolanis NC, Ng D, Pauli J, Resch-Genger U, Kuhn E, 
            Heuer S, Beisker W, Koster RW, Zitzelsberger H, Caldwell RB
  TITLE     Usefulness of a Darwinian system in a biotechnological application: 
            evolution of optical window fluorescent protein variants under 
            selective pressure.
  JOURNAL   PLoS One. 2014 Sep 5;9(9):e107069. doi: 
            10.1371/journal.pone.0107069. eCollection 2014.
  PUBMED    25192257
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-09-5"; volume: "9(9):e107069. doi"; pages: " 
            10.1371/journal.pone.0107069. eCollection 2014"
COMMENT     Sequence Label: pRSETa-Amrose v1
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..702
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKESFPEGFTWERITTYEDGGVLTA
                     TQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMMYPADSGLRGHNQMALK
                     LVGGGYLHCSLKTTYRSKKPAKNLKMPGFHFVDYRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 ggatccatga gcgagctgat caaggagaac atgcacatga aactgtacat ggagggcacc
       61 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
      121 cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
      181 gctaccagct tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg catccccgac
      241 ttctttaagg agtccttccc tgagggcttc acatgggaga gaatcaccac atacgaagac
      301 gggggcgtgc tgaccgctac ccaggacacc agcctccaga acggctgcct catctacaac
      361 gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
      421 ggctgggagg ccagcaccga gatgatgtac cccgctgaca gcggcctgag aggccataac
      481 cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccctcaa gaccacatac
      541 agatccaaga aacccgctaa gaacctcaag atgcccggct tccacttcgt ggactacaga
      601 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg
      661 gccaagtact gcgacctccc tagcaaactg gggcacagat gactcgagat ct
//
