LOCUS       sequence-148686-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of 
            yeast genes with yeast-adapted mTagBFP and G418 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pIM573
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..805
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="KEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDA
                     PELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQ
                     VLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFD
                     DERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRY
                     QDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(63..82)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      811..1008
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 taaggaaaag actcacgttt cgaggccgcg attaaattcc aacatggatg ctgatttata
       61 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
      121 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
      181 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
      241 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggcaa
      301 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
      361 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
      421 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
      481 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
      541 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
      601 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
      661 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
      721 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
      781 tttgatgctc gatgagtttt tctaatcagt actgacaata aaaagattct tgttttcaag
      841 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
      901 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
      961 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
     1021 tactaacgcc gccatccagt ttaaacgagc tcgaattcat cgatga
//
