LOCUS       sequence-148689-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRSETa (Invitrogen) plasmid expressing Amrose variant 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Schoetz U, Deliolanis NC, Ng D, Pauli J, Resch-Genger U, Kuhn E, 
            Heuer S, Beisker W, Koster RW, Zitzelsberger H, Caldwell RB
  TITLE     Usefulness of a Darwinian system in a biotechnological application: 
            evolution of optical window fluorescent protein variants under 
            selective pressure.
  JOURNAL   PLoS One. 2014 Sep 5;9(9):e107069. doi: 
            10.1371/journal.pone.0107069. eCollection 2014.
  PUBMED    25192257
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-09-5"; volume: "9(9):e107069. doi"; pages: " 
            10.1371/journal.pone.0107069. eCollection 2014"
COMMENT     Sequence Label: pRSETa-Amrose v2
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..696
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKESFPEGFTWERITTYEDGGVLTA
                     TQDTSLQNGCLIYNVKINGVDFPSNGPVMQKKTLGWEASTEMMYPADSGLRGHNQMALK
                     LVGGGYLHCSLKTTYRSKKPANNLKMPGFHFVDYRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 atgagcgagc tgatcaagga gaacatgcac atgaaactgt acatggaggg caccgtgaac
       61 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      121 atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      181 agcttcatgt acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt
      241 aaggagtcct tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc
      301 gtgctgaccg ctacccagga caccagcctc cagaacggct gcctcatcta caacgtcaag
      361 atcaacgggg tggacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
      421 gaggccagca ccgagatgat gtaccccgct gacagcggcc tgagaggcca taaccagatg
      481 gccctgaagc tcgtgggcgg gggctacctg cactgctccc tcaagaccac atacagatcc
      541 aagaaacccg ctaacaacct caagatgccc ggcttccact tcgtggacta cagactggaa
      601 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag
      661 tactgcgacc tccctagcaa actggggcac agatga
//
