LOCUS       sequence-148705-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derived from 21 with "ClpX" coding sequence from Genbank U00096.3 
            substituted for "deGFP" – used as T500 template for 134-137..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             142..164
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 acgatatccg cctgatgcgt gaacgtgacg gacgtaacca ccgcgacatg tgtgtgctgt
       61 tccgctgggc atgctgagct aacaccgtgc gtgttgacaa ttttacctct ggcggtgata
      121 atggttgcag ctagcaataa ttttgtttaa ctttaagaag gagatatacc atgacagata
      181 aacgcaaaga tggctcaggc aaattgctgt attgctcttt ttgcggcaaa agccagcatg
      241 aagtgcgcaa gctgattgcc ggtccatccg tgtatatctg cgacgaatgt gttgatttat
      301 gtaacgacat cattcgcgaa gagattaaag aagttgcacc gcatcgtgaa cgcagtgcgc
      361 taccgacgcc gcatgaaatt cgcaaccacc tggacgatta cgttatcggc caggaacagg
      421 cgaaaaaagt gctggcggtc gcggtataca accattacaa acgtctgcgc aacggcgata
      481 ccagcaatgg cgtcgagttg ggcaaaagta acattctgct gatcggtccg accggttccg
      541 gtaaaacgct gctggctgaa acgctggcgc gcctgctgga tgttccgttc accatggccg
      601 acgcgactac actgaccgaa gccggttatg tgggtgaaga cgttgaaaac atcattcaga
      661 agctgttgca gaaatgcgac tacgatgtcc agaaagcaca gcgtggtatt gtctacatcg
      721 atgaaatcga caagatttct cgtaagtcag acaacccgtc cattacccga gacgtttccg
      781 gtgaaggcgt acagcaggca ctgttgaaac tgatcgaagg tacggtagct gctgttccac
      841 cgcaaggtgg gcgtaaacat ccgcagcagg aattcttgca ggt
//
