LOCUS       sequence-148717-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of 
            yeast genes with yeast-adapted TagRFP-T and nourseothricin selection
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pIM700
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..568
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="TTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTL
                     REVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRL
                     TVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLC
                     GLDTALYDGTASDGEQALYMSMPCP"
     terminator      576..763
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(631..655)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(955..974)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(956..974)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taccactctt gacgacacgg cttaccggta ccgcaccagt gtcccggggg acgccgaggc
       61 catcgaggca ctggatgggt ccttcaccac cgacaccgtc ttccgcgtca ccgccaccgg
      121 ggacggcttc accctgcggg aggtgccggt ggacccgccc ctgaccaagg tgttccccga
      181 cgacgaatcg gacgacgaat cggacgacgg ggaggacggc gacccggact cccggacgtt
      241 cgtcgcgtac ggggacgacg gcgacctggc gggcttcgtg gtcgtctcgt actccggctg
      301 gaaccgccgg ctgaccgtcg aggacatcga ggtcgccccg gagcaccggg ggcacggggt
      361 cgggcgcgcg ttgatggggc tcgcgacgga gttcgcccgc gagcggggcg ccgggcacct
      421 ctggctggag gtcaccaacg tcaacgcacc ggcgatccac gcgtaccggc ggatggggtt
      481 caccctctgc ggcctggaca ccgccctgta cgacggcacc gcctcggacg gcgagcaggc
      541 gctctacatg agcatgccct gcccctaatc tcgaggcgaa tttcttatga tttatgattt
      601 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
      661 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
      721 aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgccg agcaaatgcc
      781 tgcaaatcgc tccccatttc acccaattgt agatatgcta actccagcaa tgagttgatg
      841 aatctcggtg tgtattttat gtcctcagag gacaacacct gttgtaatcg agctcgaatt
      901 catcgatgat atcagatcca ctagtggcct atgcggccgc ggatctgccg gtctccctat
      961 agtgagtcgt attaatttcg ataagccagg ttaacctgca ttaatgaatc g
//
