LOCUS       sequence-148720-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX 
            membrane tag on end.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL
  TITLE     Optimized second-generation CRY2-CIB dimerizers and photoactivatable
            Cre recombinase.
  JOURNAL   Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.
  PUBMED    27065233
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063."
COMMENT     Sequence Label: CIB81-pmGFP
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    123..188
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(287..308)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     636..655
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta tctagatccg
      121 gtggatcccg ggcccgcggt accgtcgact gcagaattcg aagcttgagc tcgagatctg
      181 agtccggact tgtacactac ataattacac actttgtctt tgacttcttt ttcttctttt
      241 tacccttata cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      301 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
      361 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      421 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
      481 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
      541 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      601 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      661 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      721 gcttcatgtg gtcggg
//
