LOCUS       sequence-148726-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCS4+-NLS-EGFP-E2A-mCherry-CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, 
            Choi SY
  TITLE     High Cleavage Efficiency of a 2A Peptide Derived from Porcine 
            Teschovirus-1 in Human Cell Lines, Zebrafish and Mice.
  JOURNAL   PLoS One. 2011  . 6(4):e18556.
  PUBMED    21602908
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(4):e18556."
COMMENT     Sequence Label: pSYC-103
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(88..795)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(165..188)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(363..382)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     632..650
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acgactcact atagggaggc tagctcagga gagcacacac ttgcagctca tgcagccggg
       61 gccactctca tcaggagggt tcagcttctt gtacagctcg tccatgccgc cggtggagtg
      121 gcggccctcg gcgcgctcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat
      181 gtccagcttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct tggccttgta
      241 ggtggtcttg acctcggcgt cgtagtggcc gccgtccttc agcttcagcc tctgcttgat
      301 ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct cccagcccat
      361 ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt
      421 gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca cgccgccgtc
      481 ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct tcaagtagtc
      541 ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact gaggggacag
      601 gatgtcccag gcgaagggca gggggccgcc cttggtcacc ttcagcttgg cggtctgggt
      661 gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga
      721 gccctccatg tgcaccttga agcgcatgaa ctccttgatg atggccatgt tgtcctcctc
      781 gcccttgctc accatggtag atctaggtcc agggttgctc tcaacatctc cagccaattt
      841 caagag
//
