LOCUS       sequence-148731-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCS4+-NLS-EGFP-F2A-mCherry-CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, 
            Choi SY
  TITLE     High Cleavage Efficiency of a 2A Peptide Derived from Porcine 
            Teschovirus-1 in Human Cell Lines, Zebrafish and Mice.
  JOURNAL   PLoS One. 2011  . 6(4):e18556.
  PUBMED    21602908
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(4):e18556."
COMMENT     Sequence Label: pSYC-104
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..102
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             112..816
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(257..275)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     525..544
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     719..742
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctgtacaagc ttaaggagct cgcatgcgga agcggagtga aacagacttt gaattttgac
       61 cttctcaagt tggcgggaga cgtggagtcc aaccctggac ctagatctac catggtgagc
      121 aagggcgagg aggacaacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      181 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      241 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
      301 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
      361 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      421 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
      481 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
      541 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
      601 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc cgaggtcaag
      661 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
      721 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgcgccgag
      781 ggccgccact ccaccggcgg catggacgag ctgtac
//
