LOCUS       sequence-148732-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCS4+-P2A-mCherry-CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, 
            Choi SY
  TITLE     High Cleavage Efficiency of a 2A Peptide Derived from Porcine 
            Teschovirus-1 in Human Cell Lines, Zebrafish and Mice.
  JOURNAL   PLoS One. 2011  . 6(4):e18556.
  PUBMED    21602908
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(4):e18556."
COMMENT     Sequence Label: pSYC-187
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..52
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     34..51
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             179..235
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(390..408)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     658..677
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gccattctgc ctggggacgt cggagcaagc ttgatttagg tgacactata gaatacaagc
       61 tacttgttct ttttgcagga tcccatcgat tgtacagata tctggcgcgc ctaccatggg
      121 gcccggccgg cctaagatca ccggtcccgg gcttaaggag ctcgcatgcg gaagcggagc
      181 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctagatc
      241 taccatggtg agcaagggcg aggaggacaa catggccatc atcaaggagt tcatgcgctt
      301 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      361 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
      421 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
      481 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
      541 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
      601 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
      661 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
      721 cggcgccctg aagggcgaga tcaagcag
//
