LOCUS       sequence-148733-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCS4+-P2A-mCherry-CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, 
            Choi SY
  TITLE     High Cleavage Efficiency of a 2A Peptide Derived from Porcine 
            Teschovirus-1 in Human Cell Lines, Zebrafish and Mice.
  JOURNAL   PLoS One. 2011  . 6(4):e18556.
  PUBMED    21602908
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(4):e18556."
COMMENT     Sequence Label: pSYC-187
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    105..239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(142..161)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     196..215
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     244..263
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        244..262
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(412..435)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(610..629)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctgggtacc gggcccaatg cattggcgcc gcggccgcga attaaaaaac ctcccacacc
       61 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
      121 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      181 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
      241 acgtaatacg actcactata gggaggctag ctcaggagag cacacacttg cagctcatgc
      301 agccggggcc actctcatca ggagggttca gcttcttgta cagctcgtcc atgccgccgg
      361 tggagtggcg gccctcggcg cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg
      421 aggtgatgtc cagcttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
      481 ccttgtaggt ggtcttgacc tcggcgtcgt agtggccgcc gtccttcagc ttcagcctct
      541 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
      601 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
      661 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
      721 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
      781 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
      841 gggacaggat gt
//
