LOCUS       sequence-148738-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of mitoLbNOX 
            which is a water-forming NADH oxidase that can be used to increase 
            NAD+/NADH ratio in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Titov DV, Cracan V, Goodman RP, Peng J, Grabarek Z, Mootha VK
  TITLE     Complementation of mitochondrial electron transport chain by 
            manipulation of the NAD+/NADH ratio.
  JOURNAL   Science. 2016 Apr 8;352(6282):231-5. doi: 10.1126/science.aad4017. 
            Epub 2016 Apr 7.
  PUBMED    27124460
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aad4017"; journalName: "Science"; date: "2016-04-8-
            8"; volume: "352"; issue: "6282"; pages: "231-5"
COMMENT     Sequence Label: pUC57-mitoLbNOX
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..38)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccaagacgcg tttacttgtc atcgtcatcc ttgtaatcca tagatccacc agatccacca
       61 gatccaccag cgttgacgga ttgggccact ttagcttggg cagcctgggc gagaatgttc
      121 aggtagttga agggcctgtc gaaattgggc tggaacagca tgtccaccat ggccaggtca
      181 tcgatggtgt tctcgttctg gatgcagacg gacagggtat tagcgctctg ggacacgtcg
      241 tatttggaca tcagggcgcc tcccaggatt ctgtgtgtat cggggtcgaa gaccaggctc
      301 atgaggacgg gctcggtgga aggcatgaac tcgggcctat agttgtcctc cacgatgacc
      361 tgctcggcat tgaggccttg ttgcttggcg gcggccagtg tgaggcctgt ggagacaatt
      421 gtcctgtcat agagggccag tccggagctg gactgggttc ccatgtactt cactgtgggc
      481 ttgacgaggt tctttccgac gaggattccc tgcctgacgg cgttggtagc gaggggaatg
      541 taggcgtttt ggtgtgtggg gttgtaatgg acggcggcgg agtcgccagc agcgaaaata
      601 tcagggttgc tggacctcat gtagtcgtcg gtaatgatgg cgccgttagg ggccatgtcc
      661 actttgcctt tcagcaggtc tgtgttgggc ctaaagccaa tgcagaggat ggccaggtcg
      721 gtctcatagg agttcttgtc tgtcttgatt gtgaggccgg tggcgctgtc ggtgaagctc
      781 tccactgtct cgcccagagc gagttgcacg ccatggtccc tgtagtcctg ttcgatgacg
      841 tcggtgaagt cggcatcgaa gtacttgggc atgaccctag ccatggcgtc aatcagggtg
      901 acgtcgtggc ctgttgtgga gtaggcctcg gccagttcgg ctccgatgta tccggcgccg
      961 atgacggtga tcctcttggc ctccttggcg tcctcaatca gagcctgggc gtgggcccaa
     1021 ttcttgcaga gctt
//
