LOCUS       sequence-148745-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  alias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast
            genes with yeast-adapted mTagBFP and nourseothricin selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pIM701
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..565
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="TLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTLR
                     EVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRLT
                     VEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTLCG
                     LDTALYDGTASDGEQALYMSMPCP"
     terminator      573..760
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(628..652)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(952..971)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(953..971)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cactcttgac gacacggctt accggtaccg caccagtgtc ccgggggacg ccgaggccat
       61 cgaggcactg gatgggtcct tcaccaccga caccgtcttc cgcgtcaccg ccaccgggga
      121 cggcttcacc ctgcgggagg tgccggtgga cccgcccctg accaaggtgt tccccgacga
      181 cgaatcggac gacgaatcgg acgacgggga ggacggcgac ccggactccc ggacgttcgt
      241 cgcgtacggg gacgacggcg acctggcggg cttcgtggtc gtctcgtact ccggctggaa
      301 ccgccggctg accgtcgagg acatcgaggt cgccccggag caccgggggc acggggtcgg
      361 gcgcgcgttg atggggctcg cgacggagtt cgcccgcgag cggggcgccg ggcacctctg
      421 gctggaggtc accaacgtca acgcaccggc gatccacgcg taccggcgga tggggttcac
      481 cctctgcggc ctggacaccg ccctgtacga cggcaccgcc tcggacggcg agcaggcgct
      541 ctacatgagc atgccctgcc cctaatctcg aggcgaattt cttatgattt atgattttta
      601 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
      661 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
      721 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccgagc aaatgcctgc
      781 aaatcgctcc ccatttcacc caattgtaga tatgctaact ccagcaatga gttgatgaat
      841 ctcggtgtgt attttatgtc ctcagaggac aacacctgtt gtaatcgagc tcgaattcat
      901 cgatgatatc agatccacta gtggcctatg cggccgcgga tctgccggtc tccctatagt
      961 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc caacgcg
//
