LOCUS       sequence-148746-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRFP control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Hong AL, Tseng YY, Cowley GS, Jonas O, Cheah JH, Kynnap BD, Doshi 
            MB, Oh C, Meyer SC, Church AJ, Gill S, Bielski CM, Keskula P, 
            Imamovic A, Howell S, Kryukov GV, Clemons PA, Tsherniak A, Vazquez 
            F, Crompton BD, Shamji AF, Rodriguez-Galindo C, Janeway KA, Roberts 
            CW, Stegmaier K, van Hummelen P, Cima MJ, Langer RS, Garraway LA, 
            Schreiber SL, Root DE, Hahn WC, Boehm JS
  TITLE     Integrated genetic and pharmacologic interrogation of rare cancers.
  JOURNAL   Nat Commun. 2016 Jun 22;7:11987. doi: 10.1038/ncomms11987.
  PUBMED    27329820
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-06-22"; volume: "7:11987. doi"; pages: " 
            10.1038/ncomms11987"
COMMENT     Sequence Label: TRCN0000231725
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(128..148)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     625..644
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(729..747)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ttacaatttt gtaatccaga ggttgattgt cgacttaacg cgttcaggca ccgggcttgc
       61 gggtcatgca ccaggatgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag
      121 ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc
      181 ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc
      241 tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc
      301 ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc
      361 atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag
      421 accgccacgg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc
      481 gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg
      541 tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg
      601 tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggtaag ctccggtgga
      661 tccccctggg gagagaggtc ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc
      721 tccggaggct tgcagaatgc ggaacaccgc gcgggcagga acagggcc
//
