LOCUS       sequence-148750-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric plasmid to construct episomal C-terminal fusions of 
            genes with yeast-adapted TagRFP-T and HIS3 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pIM23
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      767..1013
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(783..801)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 agcttatcga taccgtcgac atggtatcta agggtgaaga attgattaag gaaaacatgc
       61 acatgaagtt gtatatggaa ggtactgtaa ataaccacca ttttaagtgt acctctgaag
      121 gtgaaggtaa accatatgaa ggtacacaaa ccatgagaat taaagttgta gaaggtggtc
      181 cattgccttt tgccttcgat atcttagcta catcttttat gtacggttca agaaccttca
      241 tcaatcatac tcaaggtata ccagatttct ttaagcaatc attccctgaa ggtttcactt
      301 gggaaagagt cactacatat gaagacggtg gtgttttgac tgccacacaa gatacatcct
      361 tgcaagacgg ttgtttaatc tataacgtta agatcagagg tgtaaacttc ccaagtaacg
      421 gtcctgtaat gcaaaagaaa actttgggtt gggaagcaaa caccgaaatg ttgtatcctg
      481 ccgatggtgg tttagaaggt cgaactgaca tggcattgaa attagttggt ggtggtcact
      541 taatttgcaa tttcaagacc acttacagat ctaaaaagcc agctaaaaac ttgaagatgc
      601 ctggtgtata ctacgtcgat catagattgg aaagaattaa agaagcagac aaggaaacat
      661 atgttgaaca acacgaagtc gctgttgcaa gatactgcga tttgccttcc aagttaggtc
      721 ataagttgaa cggtatggac gaattgtata agtaaaactg gtcgagtcat gtaattagtt
      781 atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt
      841 agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg
      901 ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt
      961 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgtggccgg
     1021 taccc
//
