LOCUS       sequence-148752-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric plasmid to construct episomal C-terminal fusions of 
            genes with yeast-adapted TagRFP-T and HIS3 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pIM23
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        128..315
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             316..978
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 tcccgtttta agagcttggt gagcgctagg agtcactgcc aggtatcgtt tgaacacggc
       61 attagtcagg gaagtcataa cacagtcctt tcccgcaatt ttctttttct attactcttg
      121 gcctcctcta gtacactcta tattttttta tgcctcggta atgattttca tttttttttt
      181 tccacctagc ggatgactct ttttttttct tagcgattgg cattatcaca taatgaatta
      241 tacattatat aaagtaatgt gatttcttcg aagaatatac taaaaaatga gcaggcaaga
      301 taaacgaagg caaagatgac agagcagaaa gccctagtaa agcgtattac aaatgaaacc
      361 aagattcaga ttgcgatctc tttaaagggt ggtcccctag cgatagagca ctcgatcttc
      421 ccagaaaaag aggcagaagc agtagcagaa caggccacac aatcgcaagt gattaacgtc
      481 cacacaggta tagggtttct ggaccatatg atacatgctc tggccaagca ttccggctgg
      541 tcgctaatcg ttgagtgcat tggtgactta cacatagacg accatcacac cactgaagac
      601 tgcgggattg ctctcggtca agcttttaaa gaggccctag gggccgtgcg tggagtaaaa
      661 aggtttggat caggatttgc gcctttggat gaggcacttt ccagagcggt ggtagatctt
      721 tcgaacaggc cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc
      781 tcttgcgaga tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc
      841 cacgttgatt gtctgcgagg caagaatgat catcaccgta gtgagagtgc gttcaaggct
      901 cttgcggttg ccataagaga agccacctcg cccaatggta ccaacgatgt tccctccacc
      961 aaaggtgttc ttatgtagtg acaccgatta tt
//
