LOCUS       sequence-148762-        9575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Output plasmid: contains 5-state register that regulates expression 
            of GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR187
FEATURES             Location/Qualifiers
     source          1..9575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(415..435)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     1030..1058
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             1094..1102
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_RNA        complement(1145..1194)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      complement(1514..1585)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    1892..1940
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    2385..2442
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     misc_feature    2919..3392
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(3465..4436)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(4436..5611)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    5937..6187
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(6190..6945)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      7036..7255
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      7323..7854
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7331..7945
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        8803..8905
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8906..9565
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8972..8991)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 taagtgccca ttcagccagt ataaccagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctgtca gccaggaagg tataacagga aaaattaaag gggagataaa atcccccctt
      121 tttggttaac tcgaggattc gaaaaggtga aatctccttt cacggcccat tcctcatgga
      181 tgggccgttt atttccccga gccagacgtc caccatacct tcgatattcg cgcccactct
      241 cccattagtc ggagtggatg tgttgcgatt gcccgctaga tcaatgtgca cgtcccttcc
      301 ccttgataaa gcaccctttg gtcgaaaaaa aaagcccgca ctgtcaggtg cgggcttttt
      361 tctgtgtttc cttatttgta gagatcatcc atgccatgtg taatcccagc agctgttaca
      421 aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt
      481 gtggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt
      541 tgataatggt ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat tttgaagtta
      601 actttgattc cattcttttg tttgtctgcc atgatgtata cattgtgtga gttatagttg
      661 tattccaatt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ttttaactcg
      721 attctattaa caagggtatc accttcaaac ttgacttcag cacgtgtctt gtagttcccg
      781 tcatctttga aaaatatagt tctttcctgt acataacctt cgggcatggc actcttgaaa
      841 aagtcatgct gtttcatatg atctgggtat ctcgcaaagc attgaacacc ataaccgaaa
      901 gtagtgacaa gtgttggcca tggaacaggt agttttccag tagtgcaaat aaatttaagg
      961 gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca
     1021 ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct
     1081 ttactcatta gtgacctcct taatataggt cggcttcgac cttaaacaaa attatttgta
     1141 gaggctgttt cgtcctcacg gactcatcag accggaaagc acatccggtg acagctgtgc
     1201 catccctcgt cctcacgagt gcggtattcc tctgggcatg ccaacacaat taacatctca
     1261 atcaaggtaa atgctttttg ctttttttgc cgtggtgcgg atctagctac cccaccgggt
     1321 agctcaatgc gcgcaataaa aaagcccccg gaaggtgatc ttccgggggc tttctcatgc
     1381 gttggtgttc actcccattc gggttgagaa gagaaaagaa aaccgccgat cctgtccacc
     1441 gcattactgc aaggtagtgg acaagaccgg cggtcttaag ttttttggct gaacttaagc
     1501 tgcgagtggg cccgagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     1561 tcgactgagc ctttcgtttt atttgatgcc tggggagtgt gagtgggagg tctgtcacag
     1621 ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct ggataacttt
     1681 acgggcatgc ataaggctcg tataatatat tcagggagac cacaacggtt tccctctaca
     1741 aataattttg tttaactttc tgaatcaatc ggcggcgcgc acttgagtcc acggctgatg
     1801 ccaacacaat taacatcaga atcaaggtaa atgctttttg ctttttttgc gggctcacta
     1861 gggaatggcg tcgtccgtga cattctgtgc gcggccggct tgtcgacgac ggcggtctcc
     1921 gtcgtcagga tcatccgggc cagcggctgt ctatgccaga agatcgtccg tgaagcgttc
     1981 aacttttagc tataaaaaaa cccgccgaag cgggtttttt cgaaaattgt aatatcttag
     2041 cgggcaatcg taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
     2101 ccttaacgcc ggtaggtaat tctagtgcag acgtaaaaaa agcggcgtgg ttagccgctt
     2161 ttttaattgc cggagtacgc ctaaaccacc agcaatcggt caggtcggag tcctagaaag
     2221 gagtttttta gttaccttaa ttctaataaa cgaaataaaa actcgccgtt cgcgcgacat
     2281 gacatggcta acaccgtgcg tttgactatt ttacctctgg cggtgataat ggttgcatat
     2341 agtgaacaag gattaacgcc gaccgacaca atctccctgc tcaggtcgtg gtttgtctgg
     2401 tcaaccaccg cggtctcagt ggtgtacggt acaaaccccg acgcaatagc gccaaccatg
     2461 caccatagcc agcctgacag tagccaaagg agttttttag ttaccttaat tgaaataaac
     2521 gaaataaaaa ctcgcgcagc tgagagcgac acagttgcct cactcctagg gtgtaatagg
     2581 cactaaaaga gtgatatggc acgcgcatgt ggaacgccgt tagagggttg ctgagccatc
     2641 gtggtcctta gactcgggaa aaacaaaaac ccgccgaagc gggttaagtg cggggcggct
     2701 agcccgccac ctaagttatg agtcaggaaa aaaggcgaca gagtaatctg tcgccttttt
     2761 tctttgcttg ctttgtaatc actcgatgac caaaagtaaa aacccgccga agcgggtttt
     2821 tacgtaaaac aggtgaaact gacgctagcc ggtcacatgt caatacttgc ccttgacagg
     2881 cattgatgga atcgtagtct cacgctgata gtctgatcga caatacaagt gggaccgtgg
     2941 tcccagaccg ataatcagac cgacaacacg agtgggatcg tggtcccaga ctaataatca
     3001 gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg
     3061 ggaccgtggt tccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagac
     3121 taataatcag accgacgata cgagtgggac catggtccca gactaataat cagaccgacg
     3181 atacgagtgg gaccgtggtc ccagtctgat tatcagaccg acgatacgag tgggaccgtg
     3241 gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc
     3301 agaccgacga tacgagtggg accgtggtcc cagtctgatt atcagaccga cgatacaagt
     3361 ggaacagtgg gcccagagag aatattcagg ccagttatgc tttctggcct gtaacaaagg
     3421 acattaagta aagacagata aacgtagact aaaacgtggt cgcatcaggg tgctggcttt
     3481 tcaagttcct taagaatggc ctcaattttc tctatacact cagttggaac acgggacctg
     3541 tccaggttaa gcaccatttt atcgccctta tacaatactg tcgctccagg agcaaactga
     3601 tgtcgtgagc ttaaactagt tcttgatgca gatgacgttt taagcacaga agttaaaaga
     3661 gtgataactt cttcagcttc aaatatcacc ccagcttttt tctgctcatg aaggttagat
     3721 gcctgctgct taagtaattc ctctttatct gtaaaggctt tttgaagtgc atcacctgac
     3781 cgggcagata gttcaccggg gtgagaaaaa agagcaacaa ctgatttagg caatttggcg
     3841 gtgttgatac agcgggtaat aatcttacgt gaaatatttt ccgcatcagc cagcgcagaa
     3901 atatttccag caaattcatt ctgcaatcgg cttgcataac gctgaccacg ttcataagca
     3961 cttgttgggc gataatcgtt acccaatctg gataatgcag ccatctgctc atcatccagc
     4021 tcgccaacca gaacacgata atcactttcg gtaagtgcag cagctttacg acggcgactc
     4081 ccatcggcaa tttctatgac accagatact cttcgaccga acgccggtgt ctgttgacca
     4141 gtcagtagaa aagaagggat gagatcatcc agtgcgtcct cagtaagcag ctcctggtca
     4201 cgttcattac ctgaccatac ccgagaggtc ttctcaacac tatcaccccg gagcacttca
     4261 agagtaaact tcacatcccg accacataca ggcaaagtaa tggcattacc gcgagccatt
     4321 actcctacgc gcgcaattaa cgaatccacc atcggggcag ctggtgtcga taacgaagta
     4381 tcttcaaccg gttgagtatt gagcgtatgt tttggaataa caggcgcacg cttcattatc
     4441 taatctccca gcgtggttta atcagacgat cgaaaatttc attgcagaca ggttcccaaa
     4501 tagaaagagc atttctccag gcaccagttg aagagcgttg atcaatggcc tgttcaaaaa
     4561 cagttctcat ccggatctga cctttaccaa cttcatccgt ttcacgtaca acatttttta
     4621 gaaccatgct tccccaggca tcccgaattt gctcctccat ccacggggac tgagagccat
     4681 tactattgct gtatttggta agcaaaatac gtacatcagg ctcgaaccct ttaagatcaa
     4741 cgttcttgag cagatcacga agcatatcga aaaactgcag tgcggaggtg tagtcaaaca
     4801 actcagcagg cgtgggaaca atcagcacat cagcagcaca tacgacatta atcgtgccga
     4861 tacccaggtt aggcgcgctg tcaataacta tgacatcata gtcatgagca acagtttcaa
     4921 tggccagtcg gagcatcagg tgtggatcgg tgggcagttt accttcatca aatttgccca
     4981 ttaactcagt ttcaatacgg tgcagagcca gacaggaagg aataatgtca agccccggcc
     5041 agcaagtggg ctttattgca taagtgacat cgtccttttc cccaagatag aaaggcagga
     5101 gagtgtcttc tgcatgaata tgaagatctg gtacccatcc gtgatacatt gaggctgttc
     5161 cctgggggtc gttaccttcc acgagcaaaa cacgtagccc cttcagagcc agatcctgag
     5221 caagatgaac agaaactgag gttttgtaaa cgccaccttt atgggcagca accccgatca
     5281 ccggtggaaa tacgtcttca gcacgtcgca atcgcgtacc aaacacatca cgcatatgat
     5341 taatttgttc aattgtataa ccaacacgtt gctcaacccg tcctcgaatt tccatatccg
     5401 ggtgcggtag tcgccctgct ttctcggcat ctctgatagc ctgagaagaa accccaacta
     5461 aatccgctgc ttcacctatt ctccagcgcc gggttatttt cctcgcttcc gggctgtcat
     5521 cattaaactg tgcaatggcg atagccttcg tcatttcatg accagcgttt atgcactggt
     5581 taagtgtttc catgagtttc attctgaaca tcctttaatc attgctttgc gtttttttat
     5641 taaatcttgc aatttactgc aaagcaacaa caaaatcgca aagtcatcaa aaaaccgcaa
     5701 agttgtttaa aataagagca acactacaaa aggagataag aagagcacat acctcagtca
     5761 cttattatca ctagcgctcg ccgcagccgt gtaaccgagc atagcgagcg aactggcgag
     5821 gaagcaaaga agaactgttc tgtcagatag ctcttacgct cagcgcaaga agaaatatcc
     5881 accgtgggaa aaactccagg tagaggtaca cacgcggata gccaattcag agtaataaac
     5941 tgtgataatc aaccctcatc aatgatgacg aactaacccc cgatatcagg tcacatgacg
     6001 aagggaaaga gaaggaaatc aactgtgaca aactgccctc aaatttggct tccttaaaaa
     6061 ttacagttca aaaagtatga gaaaatccat gcaggctgaa ggaaacagca aaactgtgac
     6121 aaattaccct cagtaggtca gaacaaatgt gacgaaccac cctcaaatct gtgacagata
     6181 accctcagac tatcctgtcg tcatggaagt gatatcgcgg aaggaaaata cgatatgagt
     6241 cgtctggcgg cctttctttt tctcaatgta tgagaggcgc attggagttc tgctgttgat
     6301 ctcattaaca cagacctgca ggaagcggcg gcggaagtca ggcatacgct ggtaactttg
     6361 aggcagctgg taacgctcta tgatccagtc gattttcaga gagacgatgc ctgagccatc
     6421 cggcttacga tactgacaca gggattcgta taaacgcatg gcatacggat tggtgatttc
     6481 ttttgtttca ctaagccgaa actgcgtaaa ccggttctgt aacccgataa agaagggaat
     6541 gagatatggg ttgatatgta cactgtaaag ccctctggat ggactgtgcg cacgtttgat
     6601 aaaccaagga aaagattcat agcctttttc atcgccggca tcctcttcag ggcgataaaa
     6661 aaccacttcc ttccccgcga aactcttcaa tgcctgccgt atatccttac tggcttccgc
     6721 agaggtcaat ccgaatattt cagcatattt agcaacatgg atctcgcaga taccgtcatg
     6781 ttcctgtagg gtgccatcag attttctgat ctggtcaacg aacagataca gcatacgttt
     6841 ttgatcccgg gagagactat atgccgcctc agtgaggtcg tttgactgga cgattcgcgg
     6901 gctattttta cgtttcttgt gattgataac cgctgtttcc gccatgacag atccatgtga
     6961 agtgtgacaa gtttttagat tgtcacacta aataaaaaag agtcaataag cagggataac
     7021 tttgtgaaaa aacagcttct tctgagggca atttgtcaca gggttaaggg caatttgtca
     7081 cagacaggac tgtcatttga gggtgatttg tcacactgaa agggcaattt gtcacaacac
     7141 cttctctaga accagcatgg ataaaggcct acaaggcgct ctaaaaaaga agatctaaaa
     7201 actataaaaa aaataattat aaaaatatcc ccgtggataa gtggataacc ccaagggaag
     7261 ttttttcagg catcgtgtgt aagcagaata tataagtgct gttccctggt gcttcctcgc
     7321 tcactcgacc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc
     7381 gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta
     7441 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct
     7501 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc
     7561 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata
     7621 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca
     7681 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg
     7741 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc
     7801 tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc ggccggccgc
     7861 ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg
     7921 ccagcccagc ggcgagggca accagccgag ggcttcgccc tgtcgctcga ctgcggcgag
     7981 cactactggc tgtaaaagga cagaccacat catggttctg tgttcattag gttgttctgt
     8041 ccattgctga cataatccgc tccacttcaa cgtaacaccg cacgaagatt tctattgttc
     8101 ctgaaggcat attcaaatcg ttttcgttac cgcttgcagg catcatgaca gaacactact
     8161 tcctataaac gctacacagg ctcctgagat taataatgcg gatctctacg ataatgggag
     8221 attttcccga ctgtttcgtt cgcttctcag tggataacag ccagcttctc tgtttaacag
     8281 acaaaaacag catatccact cagttccaca tttccatata aaggccaagg catttattct
     8341 caggataatt gtttcagcat cgcaaccgca tcagactccg gcatcgcaaa ctgcacccgg
     8401 tgccgggcag ccacatccag cgcaaaaacc ttcgtgtaga cttccgttga actgatggac
     8461 ttatgtccca tcaggctttg cagaactttc agcggtatac cggcatacag catgtgcatc
     8521 gcataggaat ggcggaacgt atgtggtgtg accggaacag agaacgtcac accgtcagca
     8581 gcagcggcgg caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag
     8641 atccgcgctt tctctgtcct tcctgtgcga cggttacgcc gctccatgag cttatcgcga
     8701 ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga
     8761 taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg
     8821 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
     8881 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     8941 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     9001 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     9061 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     9121 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     9181 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     9241 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     9301 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     9361 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     9421 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     9481 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     9541 cgatgagtgg cagggcgggg cgtaagacgt ctaag
//
