LOCUS       sequence-148764-        9671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Output plasmid: contains 16-state register that regulates expression
            of GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR284
FEATURES             Location/Qualifiers
     source          1..9671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    650..707
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    935..983
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     misc_RNA        1026..1075
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             1118..1126
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(1162..1190)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1785..1805
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    2038..2095
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     misc_feature    3015..3488
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(3561..4532)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(4532..5707)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    6033..6283
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(6286..7041)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      7132..7351
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      7419..7950
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7427..8041
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        8899..9001
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             9002..9661
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9068..9087)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 taagtgccca ttcagccagt ataaccagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctgtca gccaggaagg tataacagga aaaattaaag gggagataaa atcccccctt
      121 tttggttaac tcgaggattc gaaaaggtga aatctccttt cacggcccat tcctcatgga
      181 tgggccgttt atttccccga gccagacgtc caccatacct tcgatattcg cgcccactct
      241 cccattagtc ggagtggatg tgttgcgatt gcccgctaga tcaatgtgca cgacactgct
      301 cgatccgctc gcacattgcc cgctagatca atgtgtacgt cgagtgcggt attcctctgg
      361 gccggccggc ttgtcgacga cggcgcactc cgtcgtcagg atcatccggg ccgtggtgcg
      421 gatctagcta ccaagcccat ttctaccgtg acggatcctg gtgcaaaacc tttcgcggta
      481 tggcatgata gcgccatagg ttaaaagcca gacatgagga gtgcgatgca gttttcgcac
      541 ttgagtccac ggctgatgcc aacacaatta acatcagaat caaggtaaat gctttttgct
      601 ttttttgcgg gctcactagg gaatggcgtc gtccgtgaca ttctgtgcgg tcggggtttg
      661 taccgtacac cactgagtgc gcggtggttg accagacaaa ccacgaccag cggctgtcta
      721 tgccagaagt cggtcaggtc ggagtcctag gcgagttttt atttcgttta ttagaattaa
      781 ggtaactaaa aaactccttt cgttcgcgcg acatgacatg ccgacacaat ctccctgctc
      841 agaacttttc ggatcaagct atgaaaaacg caaagaggga actaaacact tgcaatagcg
      901 ccaaccatgc accatagcca gcctgacagt agcccggccg gcttgtcgac gacggcggtc
      961 tccgtcgtca ggatcatccg ggcgcagctg agagcgacac agtgtgagga cgagggatgg
     1021 cacagctgtc accggatgtg ctttccggtc tgatgagtcc gtgaggacga aacagcctct
     1081 acaaataatt ttgtttaagg tcgaagccga cctatattaa ggaggtcact aatgagtaaa
     1141 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     1201 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     1261 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     1321 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
     1381 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     1441 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     1501 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     1561 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     1621 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     1681 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1741 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     1801 gtaacagctg ctgggattac acatggcatg gatgatctct acaaataagg aaacacagaa
     1861 aaaagcccgc acctgacagt gcgggctttt tttttcgacc aaagggtgct ttatcaaggg
     1921 gaaggcctgt acttgggatg ctggcgttta gtttctcgtt tcttcttctt ttaacgagag
     1981 aaaacgagga actaacttgg ggtgcaggta ggccatattg tcttcggggc cccactcgtc
     2041 gtggtttgtc tggtcaacca ccgcggtctc agtggtgtac ggtacaaacc ccgacgaggc
     2101 gataagctcg gcaaggctga ttacgcctgc tcgtgtgatg ccaacacaat taacatctca
     2161 atcaaggtaa atgctttttg ctttttttgc ccggatctcc ttagtgtagg ttccgactcg
     2221 cgcttcgtcg acacaacttt tcggatcaag ctatgaagga cgcaaagagg gaactaaaca
     2281 cttcgtccct gaggctgcag ttcacacagc taacaccacg tcgtccctat ctgctgccct
     2341 aggtctatga gtggttgctg gataacttta cgggcatgca taaggctcgt ataatatatt
     2401 cagggagacc acaacggttt ccctctacaa ataattttgt ttaactttct gaatcaatcg
     2461 gcggcggtac gatcgcacgc cccatgaaag gagtttttta gttaccttaa ttgaaataaa
     2521 cgaaataaaa actcgccaga tcgacaggta gaccaggtgc ggtgcgaata cgagtcgtag
     2581 ttagttcctc gttttctctc gttggaagaa gaagaaacga gaaactaaag caatgctggt
     2641 ctggctatga ctgcctcact cctagggtgt aataggcact aaaagagtga tatggcacgc
     2701 gcatgtggaa cgccgttaga gggttgctga gccatcgtgg tccttagact cgggaaaaac
     2761 aaaaacccgc cgaagcgggt taagtgcggg gcggctagcc cgccacctaa gttatgagtc
     2821 aggaaaaaag gcgacagagt aatctgtcgc cttttttctt tgcttgcttt gtaatcactc
     2881 gatgaccaaa agtaaaaacc cgccgaagcg ggtttttacg taaaacaggt gaaactgacg
     2941 ctagccggtc acatgtcaat acttgccctt gacaggcatt gatggaatcg tagtctcacg
     3001 ctgatagtct gatcgacaat acaagtggga ccgtggtccc agaccgataa tcagaccgac
     3061 aacacgagtg ggatcgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt
     3121 ggtcccagac taataatcag accgacgata cgagtgggac cgtggttcca gactaataat
     3181 cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag
     3241 tgggaccatg gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag
     3301 tctgattatc agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga
     3361 cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg
     3421 tggtcccagt ctgattatca gaccgacgat acaagtggaa cagtgggccc agagagaata
     3481 ttcaggccag ttatgctttc tggcctgtaa caaaggacat taagtaaaga cagataaacg
     3541 tagactaaaa cgtggtcgca tcagggtgct ggcttttcaa gttccttaag aatggcctca
     3601 attttctcta tacactcagt tggaacacgg gacctgtcca ggttaagcac cattttatcg
     3661 cccttataca atactgtcgc tccaggagca aactgatgtc gtgagcttaa actagttctt
     3721 gatgcagatg acgttttaag cacagaagtt aaaagagtga taacttcttc agcttcaaat
     3781 atcaccccag cttttttctg ctcatgaagg ttagatgcct gctgcttaag taattcctct
     3841 ttatctgtaa aggctttttg aagtgcatca cctgaccggg cagatagttc accggggtga
     3901 gaaaaaagag caacaactga tttaggcaat ttggcggtgt tgatacagcg ggtaataatc
     3961 ttacgtgaaa tattttccgc atcagccagc gcagaaatat ttccagcaaa ttcattctgc
     4021 aatcggcttg cataacgctg accacgttca taagcacttg ttgggcgata atcgttaccc
     4081 aatctggata atgcagccat ctgctcatca tccagctcgc caaccagaac acgataatca
     4141 ctttcggtaa gtgcagcagc tttacgacgg cgactcccat cggcaatttc tatgacacca
     4201 gatactcttc gaccgaacgc cggtgtctgt tgaccagtca gtagaaaaga agggatgaga
     4261 tcatccagtg cgtcctcagt aagcagctcc tggtcacgtt cattacctga ccatacccga
     4321 gaggtcttct caacactatc accccggagc acttcaagag taaacttcac atcccgacca
     4381 catacaggca aagtaatggc attaccgcga gccattactc ctacgcgcgc aattaacgaa
     4441 tccaccatcg gggcagctgg tgtcgataac gaagtatctt caaccggttg agtattgagc
     4501 gtatgttttg gaataacagg cgcacgcttc attatctaat ctcccagcgt ggtttaatca
     4561 gacgatcgaa aatttcattg cagacaggtt cccaaataga aagagcattt ctccaggcac
     4621 cagttgaaga gcgttgatca atggcctgtt caaaaacagt tctcatccgg atctgacctt
     4681 taccaacttc atccgtttca cgtacaacat tttttagaac catgcttccc caggcatccc
     4741 gaatttgctc ctccatccac ggggactgag agccattact attgctgtat ttggtaagca
     4801 aaatacgtac atcaggctcg aaccctttaa gatcaacgtt cttgagcaga tcacgaagca
     4861 tatcgaaaaa ctgcagtgcg gaggtgtagt caaacaactc agcaggcgtg ggaacaatca
     4921 gcacatcagc agcacatacg acattaatcg tgccgatacc caggttaggc gcgctgtcaa
     4981 taactatgac atcatagtca tgagcaacag tttcaatggc cagtcggagc atcaggtgtg
     5041 gatcggtggg cagtttacct tcatcaaatt tgcccattaa ctcagtttca atacggtgca
     5101 gagccagaca ggaaggaata atgtcaagcc ccggccagca agtgggcttt attgcataag
     5161 tgacatcgtc cttttcccca agatagaaag gcaggagagt gtcttctgca tgaatatgaa
     5221 gatctggtac ccatccgtga tacattgagg ctgttccctg ggggtcgtta ccttccacga
     5281 gcaaaacacg tagccccttc agagccagat cctgagcaag atgaacagaa actgaggttt
     5341 tgtaaacgcc acctttatgg gcagcaaccc cgatcaccgg tggaaatacg tcttcagcac
     5401 gtcgcaatcg cgtaccaaac acatcacgca tatgattaat ttgttcaatt gtataaccaa
     5461 cacgttgctc aacccgtcct cgaatttcca tatccgggtg cggtagtcgc cctgctttct
     5521 cggcatctct gatagcctga gaagaaaccc caactaaatc cgctgcttca cctattctcc
     5581 agcgccgggt tattttcctc gcttccgggc tgtcatcatt aaactgtgca atggcgatag
     5641 ccttcgtcat ttcatgacca gcgtttatgc actggttaag tgtttccatg agtttcattc
     5701 tgaacatcct ttaatcattg ctttgcgttt ttttattaaa tcttgcaatt tactgcaaag
     5761 caacaacaaa atcgcaaagt catcaaaaaa ccgcaaagtt gtttaaaata agagcaacac
     5821 tacaaaagga gataagaaga gcacatacct cagtcactta ttatcactag cgctcgccgc
     5881 agccgtgtaa ccgagcatag cgagcgaact ggcgaggaag caaagaagaa ctgttctgtc
     5941 agatagctct tacgctcagc gcaagaagaa atatccaccg tgggaaaaac tccaggtaga
     6001 ggtacacacg cggatagcca attcagagta ataaactgtg ataatcaacc ctcatcaatg
     6061 atgacgaact aacccccgat atcaggtcac atgacgaagg gaaagagaag gaaatcaact
     6121 gtgacaaact gccctcaaat ttggcttcct taaaaattac agttcaaaaa gtatgagaaa
     6181 atccatgcag gctgaaggaa acagcaaaac tgtgacaaat taccctcagt aggtcagaac
     6241 aaatgtgacg aaccaccctc aaatctgtga cagataaccc tcagactatc ctgtcgtcat
     6301 ggaagtgata tcgcggaagg aaaatacgat atgagtcgtc tggcggcctt tctttttctc
     6361 aatgtatgag aggcgcattg gagttctgct gttgatctca ttaacacaga cctgcaggaa
     6421 gcggcggcgg aagtcaggca tacgctggta actttgaggc agctggtaac gctctatgat
     6481 ccagtcgatt ttcagagaga cgatgcctga gccatccggc ttacgatact gacacaggga
     6541 ttcgtataaa cgcatggcat acggattggt gatttctttt gtttcactaa gccgaaactg
     6601 cgtaaaccgg ttctgtaacc cgataaagaa gggaatgaga tatgggttga tatgtacact
     6661 gtaaagccct ctggatggac tgtgcgcacg tttgataaac caaggaaaag attcatagcc
     6721 tttttcatcg ccggcatcct cttcagggcg ataaaaaacc acttccttcc ccgcgaaact
     6781 cttcaatgcc tgccgtatat ccttactggc ttccgcagag gtcaatccga atatttcagc
     6841 atatttagca acatggatct cgcagatacc gtcatgttcc tgtagggtgc catcagattt
     6901 tctgatctgg tcaacgaaca gatacagcat acgtttttga tcccgggaga gactatatgc
     6961 cgcctcagtg aggtcgtttg actggacgat tcgcgggcta tttttacgtt tcttgtgatt
     7021 gataaccgct gtttccgcca tgacagatcc atgtgaagtg tgacaagttt ttagattgtc
     7081 acactaaata aaaaagagtc aataagcagg gataactttg tgaaaaaaca gcttcttctg
     7141 agggcaattt gtcacagggt taagggcaat ttgtcacaga caggactgtc atttgagggt
     7201 gatttgtcac actgaaaggg caatttgtca caacaccttc tctagaacca gcatggataa
     7261 aggcctacaa ggcgctctaa aaaagaagat ctaaaaacta taaaaaaaat aattataaaa
     7321 atatccccgt ggataagtgg ataaccccaa gggaagtttt ttcaggcatc gtgtgtaagc
     7381 agaatatata agtgctgttc cctggtgctt cctcgctcac tcgaccggga gggttcgaga
     7441 agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa
     7501 aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa
     7561 aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt
     7621 caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc
     7681 tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct
     7741 tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc
     7801 tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc
     7861 gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt
     7921 ttccgcgagg tatccacaac gccggcggcc ggccgcggtg tctcgcacac ggcttcgacg
     7981 gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca
     8041 gccgagggct tcgccctgtc gctcgactgc ggcgagcact actggctgta aaaggacaga
     8101 ccacatcatg gttctgtgtt cattaggttg ttctgtccat tgctgacata atccgctcca
     8161 cttcaacgta acaccgcacg aagatttcta ttgttcctga aggcatattc aaatcgtttt
     8221 cgttaccgct tgcaggcatc atgacagaac actacttcct ataaacgcta cacaggctcc
     8281 tgagattaat aatgcggatc tctacgataa tgggagattt tcccgactgt ttcgttcgct
     8341 tctcagtgga taacagccag cttctctgtt taacagacaa aaacagcata tccactcagt
     8401 tccacatttc catataaagg ccaaggcatt tattctcagg ataattgttt cagcatcgca
     8461 accgcatcag actccggcat cgcaaactgc acccggtgcc gggcagccac atccagcgca
     8521 aaaaccttcg tgtagacttc cgttgaactg atggacttat gtcccatcag gctttgcaga
     8581 actttcagcg gtataccggc atacagcatg tgcatcgcat aggaatggcg gaacgtatgt
     8641 ggtgtgaccg gaacagagaa cgtcacaccg tcagcagcag cggcggcaac cgcctcccca
     8701 atccaggtcc tgaccgttct gtccgtcact tcccagatcc gcgctttctc tgtccttcct
     8761 gtgcgacggt tacgccgctc catgagctta tcgcgaataa atacctgtga cggaagatca
     8821 cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct gggccaactt
     8881 ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat
     8941 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
     9001 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     9061 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     9121 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     9181 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
     9241 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     9301 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     9361 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     9421 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     9481 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     9541 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     9601 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     9661 agacgtctaa g
//
