LOCUS       sequence-148766-       11262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Output plasmid: contains 5-state register that regulates expression 
            of GFP, RFP, and BFP. Expresses a unique fluorescent signature in 
            each state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 11262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR291
FEATURES             Location/Qualifiers
     source          1..11262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(415..435)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     1030..1058
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             1094..1102
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_RNA        complement(1145..1194)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             1836..1844
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    2694..2742
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             complement(2968..3642)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     RBS             3675..3683
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    3910..3967
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     misc_feature    4606..5079
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(5152..6123)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(6123..7298)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    7624..7874
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(7877..8632)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      8723..8942
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      9010..9541
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      9018..9632
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        10490..10592
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             10593..11252
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10659..10678)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 taagtgccca ttcagccagt ataaccagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctgtca gccaggaagg tataacagga aaaattaaag gggagataaa atcccccctt
      121 tttggttaac tcgaggattc gaaaaggtga aatctccttt cacggcccat tcctcatgga
      181 tgggccgttt atttccccga gccagacgtc caccatacct tcgatattcg cgcccactct
      241 cccattagtc ggagtggatg tgttgcgatt gcccgctaga tcaatgtgca cgtcccttcc
      301 ccttgataaa gcaccctttg gtcgaaaaaa aaagcccgca ctgtcaggtg cgggcttttt
      361 tctgtgtttc cttatttgta gagatcatcc atgccatgtg taatcccagc agctgttaca
      421 aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt
      481 gtggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt
      541 tgataatggt ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat tttgaagtta
      601 actttgattc cattcttttg tttgtctgcc atgatgtata cattgtgtga gttatagttg
      661 tattccaatt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ttttaactcg
      721 attctattaa caagggtatc accttcaaac ttgacttcag cacgtgtctt gtagttcccg
      781 tcatctttga aaaatatagt tctttcctgt acataacctt cgggcatggc actcttgaaa
      841 aagtcatgct gtttcatatg atctgggtat ctcgcaaagc attgaacacc ataaccgaaa
      901 gtagtgacaa gtgttggcca tggaacaggt agttttccag tagtgcaaat aaatttaagg
      961 gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca
     1021 ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct
     1081 ttactcatta gtgacctcct taatataggt cggcttcgac cttaaacaaa attatttgta
     1141 gaggctgttt cgtcctcacg gactcatcag accggaaagc acatccggtg acagctgtgc
     1201 catccctcgt cctcacgagt gcggtattcc tctgggcatg ccaacacaat taacatctca
     1261 atcaaggtaa atgctttttg ctttttttgc cgtggtgcgg atctagctac cggaacaggt
     1321 tggtttgact gagcccctca atcgaggggc ttttttttgc gtgcgggaaa atcaccgacg
     1381 aaggttgaaa aataaaaacg gcgctaaaaa gcgccgtttt ttttgacggt ggtaccgttt
     1441 tatagaagcc taggtgtcat acgtaaaaaa gggcgatctt gcgaccgccc tttttttatt
     1501 aaatgtgctt aagagggcaa actctgaagc ccatttctac cgtgacggat cctggtgcaa
     1561 aacctttcgc ggtatggcat gatagcgcca taggttaaaa gccagacatg aggagtgcga
     1621 tgcagttttc gcacttgagt ccacggctga tgccaacaca attaacatca gaatcaaggt
     1681 aaatgctttt tgcttttttt gcgggctcac tagggaatgg cggcgaggat agtgtaaacg
     1741 gagcagcggt caacgcatgt gctttgcgtt ctgatgagac agtgatgtcg aaaccgcctc
     1801 tacaaataat tttgtttaaa gtcaaaagag tatactaagg aggtaacgaa tgagcgagct
     1861 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     1921 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     1981 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     2041 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     2101 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     2161 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     2221 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     2281 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     2341 cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc
     2401 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     2461 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     2521 ccctagcaaa ctggggcact aatgtgaaaa agcccgcgca agcgggtttt tttatgtctc
     2581 agctaggtgt agttgcgcta acaccgtgcg tttgactatt ttacctctgg cggtgataat
     2641 ggttgcatat agtgaacaag gattaacgcc gatcgtccgt gacattctgt gcgcggccgg
     2701 cttgtcgacg acggcggtct ccgtcgtcag gatcatccgg gccagcggct gtctatgcca
     2761 gaagtagaac atagcgcccc acgtggggag actaaggcag ccagatggct gcctttttta
     2821 caggtgccag gtgatttcaa ctaataaaaa aaaccccggg acctttcggt gcgggggtct
     2881 tactatcccc taaacttcaa atcacaaaat aaaaaggccg gttaaaccga ccttttactc
     2941 gttcttgtcc gcacaggtgg atgcttatta agcaccggtg gagtgacgac cttcagcacg
     3001 ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca gtttgatgtc
     3061 ggttttgtaa gcacccggca gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc
     3121 agcgtcgtag tgaccaccgt ctttcagttt tagacgcatt ttgatttcac ctttcagagc
     3181 accgtcttcc gggtacatac gttcggtgga agcttcccaa cccatggttt ttttctgcat
     3241 aaccggaccg tcggacggga agttggtacc acgcagttta actttgtaga tgaactcacc
     3301 gtcttgcagg gaggagtcct gggtaacggt aacaacacca ccgtcttcga agttcataac
     3361 acgttcccat ttgaaacctt ccgggaagga cagtttcagg tagtccggga tgtcagccgg
     3421 gtgtttaacg taagctttgg aaccgtactg gaactgcggg gacaggatgt cccaagcgaa
     3481 cggcagcgga ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg
     3541 accttcacct tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatacgaac
     3601 tttgaaacgc atgaactctt tgataacgtc ttcagagctc gcaactttac ttttatctaa
     3661 tctggacatg gctaacctcc ttaagtgtac taagaattct taaacaaaat tatttgtaga
     3721 ggcgctttcg tcctcacgga ctcatcagaa cgggaagcac acccgttgag cgctccgagt
     3781 gtataaccct tcgatgtcgg tcaggtcgga gtcctagaaa ggagtttttt agttacctta
     3841 attctaataa acgaaataaa aactcgccgt tcgcgcgaca tgacatgccg acacaatctc
     3901 cctgctcagg tcgtggtttg tctggtcaac caccgcggtc tcagtggtgt acggtacaaa
     3961 ccccgacgca atagcgccaa ccatgcacca tagccagcct gacagtagcc aaaggagttt
     4021 tttagttacc ttaattgaaa taaacgaaat aaaaactcgc gcagctgaga gcgacacagt
     4081 cgtcgccgat tgattcagaa agttaaacaa aattatttgt agagggaaac cgttgtggtc
     4141 tccctgaata tattatacga gccttatgca tgcccgtaaa gttatccagc aaccactcat
     4201 agacctaggg cagcagatag ggacgacgtg gtgttagctg tgtgcctcac tcctagggtg
     4261 taataggcac taaaagagtg atatggcacg cgcatgtgga acgccgttag agggttgctg
     4321 agccatcgtg gtccttagac tcgggaaaaa caaaaacccg ccgaagcggg ttaagtgcgg
     4381 ggcggctagc ccgccaccta agttatgagt caggaaaaaa ggcgacagag taatctgtcg
     4441 ccttttttct ttgcttgctt tgtaatcact cgatgaccaa aagtaaaaac ccgccgaagc
     4501 gggtttttac gtaaaacagg tgaaactgac gctagccggt cacatgtcaa tacttgccct
     4561 tgacaggcat tgatggaatc gtagtctcac gctgatagtc tgatcgacaa tacaagtggg
     4621 accgtggtcc cagaccgata atcagaccga caacacgagt gggatcgtgg tcccagacta
     4681 ataatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat
     4741 acgagtggga ccgtggttcc agactaataa tcagaccgac gatacgagtg ggaccgtggt
     4801 cccagactaa taatcagacc gacgatacga gtgggaccat ggtcccagac taataatcag
     4861 accgacgata cgagtgggac cgtggtccca gtctgattat cagaccgacg atacgagtgg
     4921 gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact
     4981 aataatcaga ccgacgatac gagtgggacc gtggtcccag tctgattatc agaccgacga
     5041 tacaagtgga acagtgggcc cagagagaat attcaggcca gttatgcttt ctggcctgta
     5101 acaaaggaca ttaagtaaag acagataaac gtagactaaa acgtggtcgc atcagggtgc
     5161 tggcttttca agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg
     5221 ggacctgtcc aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc
     5281 aaactgatgt cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt
     5341 taaaagagtg ataacttctt cagcttcaaa tatcacccca gcttttttct gctcatgaag
     5401 gttagatgcc tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc
     5461 acctgaccgg gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa
     5521 tttggcggtg ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag
     5581 cgcagaaata tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc
     5641 ataagcactt gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc
     5701 atccagctcg ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg
     5761 gcgactccca tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg
     5821 ttgaccagtc agtagaaaag aagggatgag atcatccagt gcgtcctcag taagcagctc
     5881 ctggtcacgt tcattacctg accatacccg agaggtcttc tcaacactat caccccggag
     5941 cacttcaaga gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg
     6001 agccattact cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa
     6061 cgaagtatct tcaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt
     6121 cattatctaa tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt
     6181 tcccaaatag aaagagcatt tctccaggca ccagttgaag agcgttgatc aatggcctgt
     6241 tcaaaaacag ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca
     6301 ttttttagaa ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga
     6361 gagccattac tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta
     6421 agatcaacgt tcttgagcag atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag
     6481 tcaaacaact cagcaggcgt gggaacaatc agcacatcag cagcacatac gacattaatc
     6541 gtgccgatac ccaggttagg cgcgctgtca ataactatga catcatagtc atgagcaaca
     6601 gtttcaatgg ccagtcggag catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat
     6661 ttgcccatta actcagtttc aatacggtgc agagccagac aggaaggaat aatgtcaagc
     6721 cccggccagc aagtgggctt tattgcataa gtgacatcgt ccttttcccc aagatagaaa
     6781 ggcaggagag tgtcttctgc atgaatatga agatctggta cccatccgtg atacattgag
     6841 gctgttccct gggggtcgtt accttccacg agcaaaacac gtagcccctt cagagccaga
     6901 tcctgagcaa gatgaacaga aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc
     6961 ccgatcaccg gtggaaatac gtcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc
     7021 atatgattaa tttgttcaat tgtataacca acacgttgct caacccgtcc tcgaatttcc
     7081 atatccgggt gcggtagtcg ccctgctttc tcggcatctc tgatagcctg agaagaaacc
     7141 ccaactaaat ccgctgcttc acctattctc cagcgccggg ttattttcct cgcttccggg
     7201 ctgtcatcat taaactgtgc aatggcgata gccttcgtca tttcatgacc agcgtttatg
     7261 cactggttaa gtgtttccat gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt
     7321 tttttattaa atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa
     7381 accgcaaagt tgtttaaaat aagagcaaca ctacaaaagg agataagaag agcacatacc
     7441 tcagtcactt attatcacta gcgctcgccg cagccgtgta accgagcata gcgagcgaac
     7501 tggcgaggaa gcaaagaaga actgttctgt cagatagctc ttacgctcag cgcaagaaga
     7561 aatatccacc gtgggaaaaa ctccaggtag aggtacacac gcggatagcc aattcagagt
     7621 aataaactgt gataatcaac cctcatcaat gatgacgaac taacccccga tatcaggtca
     7681 catgacgaag ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc
     7741 ttaaaaatta cagttcaaaa agtatgagaa aatccatgca ggctgaagga aacagcaaaa
     7801 ctgtgacaaa ttaccctcag taggtcagaa caaatgtgac gaaccaccct caaatctgtg
     7861 acagataacc ctcagactat cctgtcgtca tggaagtgat atcgcggaag gaaaatacga
     7921 tatgagtcgt ctggcggcct ttctttttct caatgtatga gaggcgcatt ggagttctgc
     7981 tgttgatctc attaacacag acctgcagga agcggcggcg gaagtcaggc atacgctggt
     8041 aactttgagg cagctggtaa cgctctatga tccagtcgat tttcagagag acgatgcctg
     8101 agccatccgg cttacgatac tgacacaggg attcgtataa acgcatggca tacggattgg
     8161 tgatttcttt tgtttcacta agccgaaact gcgtaaaccg gttctgtaac ccgataaaga
     8221 agggaatgag atatgggttg atatgtacac tgtaaagccc tctggatgga ctgtgcgcac
     8281 gtttgataaa ccaaggaaaa gattcatagc ctttttcatc gccggcatcc tcttcagggc
     8341 gataaaaaac cacttccttc cccgcgaaac tcttcaatgc ctgccgtata tccttactgg
     8401 cttccgcaga ggtcaatccg aatatttcag catatttagc aacatggatc tcgcagatac
     8461 cgtcatgttc ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac agatacagca
     8521 tacgtttttg atcccgggag agactatatg ccgcctcagt gaggtcgttt gactggacga
     8581 ttcgcgggct atttttacgt ttcttgtgat tgataaccgc tgtttccgcc atgacagatc
     8641 catgtgaagt gtgacaagtt tttagattgt cacactaaat aaaaaagagt caataagcag
     8701 ggataacttt gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg ttaagggcaa
     8761 tttgtcacag acaggactgt catttgaggg tgatttgtca cactgaaagg gcaatttgtc
     8821 acaacacctt ctctagaacc agcatggata aaggcctaca aggcgctcta aaaaagaaga
     8881 tctaaaaact ataaaaaaaa taattataaa aatatccccg tggataagtg gataacccca
     8941 agggaagttt tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct
     9001 tcctcgctca ctcgaccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg
     9061 gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt ggtttaaaag
     9121 caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg caaatgctgg
     9181 attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac
     9241 tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt
     9301 gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg aaactcgcgt
     9361 aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc
     9421 gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt
     9481 ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa cgccggcggc
     9541 cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag
     9601 acggccgcca gcccagcggc gagggcaacc agccgagggc ttcgccctgt cgctcgactg
     9661 cggcgagcac tactggctgt aaaaggacag accacatcat ggttctgtgt tcattaggtt
     9721 gttctgtcca ttgctgacat aatccgctcc acttcaacgt aacaccgcac gaagatttct
     9781 attgttcctg aaggcatatt caaatcgttt tcgttaccgc ttgcaggcat catgacagaa
     9841 cactacttcc tataaacgct acacaggctc ctgagattaa taatgcggat ctctacgata
     9901 atgggagatt ttcccgactg tttcgttcgc ttctcagtgg ataacagcca gcttctctgt
     9961 ttaacagaca aaaacagcat atccactcag ttccacattt ccatataaag gccaaggcat
    10021 ttattctcag gataattgtt tcagcatcgc aaccgcatca gactccggca tcgcaaactg
    10081 cacccggtgc cgggcagcca catccagcgc aaaaaccttc gtgtagactt ccgttgaact
    10141 gatggactta tgtcccatca ggctttgcag aactttcagc ggtataccgg catacagcat
    10201 gtgcatcgca taggaatggc ggaacgtatg tggtgtgacc ggaacagaga acgtcacacc
    10261 gtcagcagca gcggcggcaa ccgcctcccc aatccaggtc ctgaccgttc tgtccgtcac
    10321 ttcccagatc cgcgctttct ctgtccttcc tgtgcgacgg ttacgccgct ccatgagctt
    10381 atcgcgaata aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc
    10441 ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg
    10501 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt
    10561 tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
    10621 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
    10681 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
    10741 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
    10801 atccggaatt tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
    10861 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
    10921 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
    10981 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
    11041 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
    11101 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
    11161 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
    11221 agtactgcga tgagtggcag ggcggggcgt aagacgtcta ag
//
