LOCUS       sequence-148780-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of shRNA for mouse Tet3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Montagner S, Leoni C, Emming S, Della Chiara G, Balestrieri C, 
            Barozzi I, Piccolo V, Togher S, Ko M, Rao A, Natoli G, Monticelli S
  TITLE     TET2 Regulates Mast Cell Differentiation and Proliferation through 
            Catalytic and Non-catalytic Activities.
  JOURNAL   Cell Rep. 2016 May 17;15(7):1566-79. doi: 
            10.1016/j.celrep.2016.04.044. Epub 2016 May 5.
  PUBMED    27160912
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.044"; journalName: "Cell Rep"; date: 
            "2016-05-17- 17"; volume: "15"; issue: "7"; pages: "1566-79"
COMMENT     Sequence Label: pAGM_Puro_shRNA n.2_mTet3
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(1..127)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(153..167)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(193..314)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(447..467)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 aaagtgattt aatttatacc attttaattc agctttgtaa aaatgtatca aagagatagc
       61 aaggtattca gttttagtaa acaagataat tgctcctaaa gtagcccctt gaattccgag
      121 gcagtaggca gctccaacga gaagctattt tacatctgtg gcttcactaa aatagcttct
      181 cgttggagcc gctcactgtc aacagcaata taccttctcg agccttctgt tgggttaacc
      241 tgaagaagta atcccagcaa gtgtttccaa gatgtgcagg caacgattct gtaaagtact
      301 gaagcctcat tcaacatagt atatgtgctg ccgaagcgag cacttaacaa ggcttgcggc
      361 cgctcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
      421 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
      481 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
      541 gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc
      601 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
      661 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
      721 gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc
      781 gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg
      841 gcggtccggg tc
//
