LOCUS       sequence-148792-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric plasmid to construct episomal C-terminal fusions of 
            genes with yeast-adapted TagRFP-T and URA3 selection marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Malcova I, Farkasovsky M, Senohrabkova L, Vasicova P, Hasek J
  TITLE     New integrative modules for multicolor-protein labeling and 
            live-cell imaging in Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2016 Mar 17. pii: fow027.
  PUBMED    26994102
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. 2016 Mar 17. pii: fow027."
COMMENT     Sequence Label: pIM35
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             213..1016
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat ttttttgatt
       61 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
      121 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
      181 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
      241 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
      301 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
      361 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
      421 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
      481 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
      541 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
      601 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
      661 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
      721 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
      781 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
      841 gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca
      901 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
      961 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     1021 ctgtat
//
